CHP (gen)

Kalcineurin B homologni protein 1 je protein koji je kod ljudi kodiran genom CHP1 (ranija CHP).^[5]

CHP (gen)
Dostupne strukture PDB Pretraga ortologa: PDBe RCSB Spisak PDB ID kodova 2E30
Identifikatori
Aliasi	CHP1
Vanjski ID-jevi	OMIM: 606988 MGI: 1927185 HomoloGene: 5235 GeneCards: CHP1
Lokacija gena (čovjek) Hrom. Hromosom 15 (čovjek)[1] Bend 15q15.1 Početak 41,230,839 bp[1] Kraj 41,281,887 bp[1]
Lokacija gena (miš) Hrom. Hromosom 2 (miš)[2] Bend 2|2 E5 Početak 119,378,178 bp[2] Kraj 119,417,508 bp[2]
Obrazac RNK ekspresije Više referentnih podataka o ekspresiji
Ontologija gena Molekularna funkcija • calcium ion binding • transporter activity • microtubule binding • protein kinase inhibitor activity • vezivanje iona metala • calcium-dependent protein binding • potassium channel regulator activity • kinase binding • GO:0001948, GO:0016582 vezivanje za proteine Ćelijska komponenta • citoplazma • citosol • membrana • focal adhesion • microtubule cytoskeleton • Golđijeva membrana • ćelijska membrana • transport vesicle • Endoplazmatski retikulum • Egzosom • citoskelet • Endomembranski sistem • jedro • endoplasmic reticulum-Golgi intermediate compartment Biološki proces • GO:0033128 negative regulation of protein phosphorylation • GO:0090284 positive regulation of protein glycosylation • negative regulation of calcineurin-NFAT signaling cascade • hyaluronan catabolic process • negative regulation of protein autophosphorylation • negative regulation of protein kinase activity • positive regulation of sodium:proton antiporter activity • negative regulation of phosphatase activity • small GTPase mediated signal transduction • protein stabilization • cellular response to acidic pH • membrane fusion • regulation of intracellular pH • positive regulation of protein transport • negative regulation of protein ubiquitination • potassium ion transport • protein complex oligomerization • microtubule bundle formation • cytoplasmic microtubule organization • protein export from nucleus • membrane docking • regulation of neuron death • protein transport • membrane organization • negative regulation of NF-kappaB transcription factor activity • calcium-ion regulated exocytosis • positive regulation of protein targeting to membrane • negative regulation of protein import into nucleus • GO:0015915 transport Izvori:Amigo / QuickGO
Ortolozi
Vrste	Čovjek	Miš
Entrez	11261	56398
Ensembl	ENSG00000187446	ENSMUSG00000014077
UniProt	Q99653	P61022
RefSeq (mRNK)	NM_007236	NM_019769
RefSeq (bjelančevina)	NP_009167	NP_062743
Lokacija (UCSC)	Chr 15: 41.23 – 41.28 Mb	Chr 2: 119.38 – 119.42 Mb
PubMed pretraga	[3]	[4]
Wikipodaci
Pogledaj/uredi – čovjek Pogledaj/uredi – miš

Gen CHP1 kodira bitni kofaktor SLC9A1 (NHE1), izmjenjivača Na+/H+ koji regulira unutarćelijske ione i homeostazu pH. Međunarodni radijacijski konzorcij za hibridno mapiranje locirao je gen CHP na hromosom 15 (stSG38846).

Kloniranje i ekspresija

Više signalnih puteva regulira Na+/H+ proteine ​​koji se transportuju. Da bi identificirali regulatorne proteine, Lin i Barber (1996) koristili su C-terminalni citoplazmatski domen sveprisutno eksprimirane izoforme NHE1 za pregled biblioteke ekspresije ljudskih B-ćelija. Djelimična sekvenca dobijena s ovog snimka korištena je za kloniranje nove cDNK pune dužine, označene kao CHP, iz druge B-ćelijske biblioteke. Izvedeni protein od 195 aminokiselina sadrži dva različita i dva potencijalna motiva vezana za [kalcij|[Ca⁽²⁺⁾ za EF-šaku i dijeli 43% i 33% identiteta s kalcineurinom B i kalmodulinom. Također ima 99% identiteta sekvence sa homologom pacova. Northern blot analiza pokazala je ekspresiju 4-kb CHP transkripta u svih osam ispitivanih fetush tkiva.

U mišjem malom mozgu, pronašli su ekspresiju gena Chp1 na presinapsnim terminalima Purkinjeovih ćelija, kao i na aksonskim terminalima drugih neurona.

Aminokiselinska sekvenca

Dužina polipeptidnog lanca je 195 aminokiselina, a molekulska težina 22.456 Da.^[6].

Simboli

C: Cistein
D: Asparaginska kiselina
E: Glutaminska kiselina
F: Fenilalanin
G: Glicin
H: Histidin
I: Izoleucin
K: Lizin
L: Leucin
M: Metionin
N: Asparagin
P: Prolin
Q: Glutamin
R: Arginin
S: Serin
T: Treonin
V: Valin
W: Triptofan
Y: Tirozin

10		20		30		40		50
MGSRASTLLR		DEELEEIKKE		TGFSHSQITR		LYSRFTSLDK		GENGTLSRED
FQRIPELAIN		PLGDRIINAF		FPEGEDQVNF		RGFMRTLAHF		RPIEDNEKSK
DVNGPEPLNS		RSNKLHFAFR		LYDLDKDEKI		SRDELLQVLR		MMVGVNISDE
QLGSIADRTI		QEADQDGDSA		ISFTEFVKVL		EKVDVEQKMS		IRFLH

Funkcija

Protein koji kodira ovaj gen je fosfoprotein koji se veže za izmjenjivače natrij-vodik (NHE). Ovaj protein služi kao esencijalni kofaktor koji podržava fiziološku aktivnost članova porodice NHE. Ima sličnost proteinske sekvence s kalcineurinom B, a poznato je i da je endogeni inhibitor aktivnosti kalcineurina.^[5]

Interakcije

Pokazalo se da homologni protein 1 kalcineurina B ima interakcija sa

• natrij-vodik antiporterom 1,^[7][8][9]
• natrij-vodik antiporterom 3,]],^[7] i
• natrij-vodik antiporterom 9.^[10]

Reference

1. GRCh38: Ensembl release 89: ENSG00000187446 - Ensembl, maj 2017
2. GRCm38: Ensembl release 89: ENSMUSG00000014077 - Ensembl, maj 2017
3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
5. "Entrez Gene: CHP calcium binding protein P22".
6. "UniProt, Q99653". Pristupljeno 12. 7. 2021.
7. Inoue H, Nakamura Y, Nagita M, Takai T, Masuda M, Nakamura N, Kanazawa H (Feb 2003). "Calcineurin homologous protein isoform 2 (CHP2), Na+/H+ exchangers-binding protein, is expressed in intestinal epithelium". Biological & Pharmaceutical Bulletin. 26 (2): 148–55. doi:10.1248/bpb.26.148. PMID 12576672.
8. Lin X, Barber DL (Oct 1996). "A calcineurin homologous protein inhibits GTPase-stimulated Na-H exchange". Proceedings of the National Academy of Sciences of the United States of America. 93 (22): 12631–6. doi:10.1073/pnas.93.22.12631. PMC 38044. PMID 8901634.
9. Pang T, Su X, Wakabayashi S, Shigekawa M (maj 2001). "Calcineurin homologous protein as an essential cofactor for Na+/H+ exchangers". The Journal of Biological Chemistry. 276 (20): 17367–72. doi:10.1074/jbc.M100296200. PMID 11350981.
10. Zhang-James Y, DasBanerjee T, Sagvolden T, Middleton FA, Faraone SV (Dec 2011). "SLC9A9 mutations, gene expression, and protein-protein interactions in rat models of attention-deficit/hyperactivity disorder". American Journal of Medical Genetics Part B. 156B (7): 835–43. doi:10.1002/ajmg.b.31229. PMC 3168688. PMID 21858920.

Dopunsdka literatura

• Robertson NG, Khetarpal U, Gutiérrez-Espeleta GA, Bieber FR, Morton CC (Sep 1994). "Isolation of novel and known genes from a human fetal cochlear cDNA library using subtractive hybridization and differential screening". Genomics. 23 (1): 42–50. doi:10.1006/geno.1994.1457. PMID 7829101.
• Barroso MR, Bernd KK, DeWitt ND, Chang A, Mills K, Sztul ES (Apr 1996). "A novel Ca2+-binding protein, p22, is required for constitutive membrane traffic". The Journal of Biological Chemistry. 271 (17): 10183–7. doi:10.1074/jbc.271.17.10183. PMID 8626580.
• Lin X, Barber DL (Oct 1996). "A calcineurin homologous protein inhibits GTPase-stimulated Na-H exchange". Proceedings of the National Academy of Sciences of the United States of America. 93 (22): 12631–6. doi:10.1073/pnas.93.22.12631. PMC 38044. PMID 8901634.
• Lin X, Sikkink RA, Rusnak F, Barber DL (Dec 1999). "Inhibition of calcineurin phosphatase activity by a calcineurin B homologous protein". The Journal of Biological Chemistry. 274 (51): 36125–31. doi:10.1074/jbc.274.51.36125. PMID 10593895.
• Pang T, Su X, Wakabayashi S, Shigekawa M (maj 2001). "Calcineurin homologous protein as an essential cofactor for Na+/H+ exchangers". The Journal of Biological Chemistry. 276 (20): 17367–72. doi:10.1074/jbc.M100296200. PMID 11350981.
• Matsumoto M, Miyake Y, Nagita M, Inoue H, Shitakubo D, Takemoto K, Ohtsuka C, Murakami H, Nakamura N, Kanazawa H (Aug 2001). "A serine/threonine kinase which causes apoptosis-like cell death interacts with a calcineurin B-like protein capable of binding Na(+)/H(+) exchanger". Journal of Biochemistry. 130 (2): 217–25. doi:10.1093/oxfordjournals.jbchem.a002975. PMID 11481038.
• Nakamura N, Miyake Y, Matsushita M, Tanaka S, Inoue H, Kanazawa H (Sep 2002). "KIF1Bbeta2, capable of interacting with CHP, is localized to synaptic vesicles". Journal of Biochemistry. 132 (3): 483–91. doi:10.1093/oxfordjournals.jbchem.a003246. PMID 12204119.
• Pang T, Wakabayashi S, Shigekawa M (Nov 2002). "Expression of calcineurin B homologous protein 2 protects serum deprivation-induced cell death by serum-independent activation of Na+/H+ exchanger". The Journal of Biological Chemistry. 277 (46): 43771–7. doi:10.1074/jbc.M208313200. PMID 12226101.
• Inoue H, Nakamura Y, Nagita M, Takai T, Masuda M, Nakamura N, Kanazawa H (Feb 2003). "Calcineurin homologous protein isoform 2 (CHP2), Na+/H+ exchangers-binding protein, is expressed in intestinal epithelium". Biological & Pharmaceutical Bulletin. 26 (2): 148–55. doi:10.1248/bpb.26.148. PMID 12576672.
• Pang T, Hisamitsu T, Mori H, Shigekawa M, Wakabayashi S (Mar 2004). "Role of calcineurin B homologous protein in pH regulation by the Na+/H+ exchanger 1: tightly bound Ca2+ ions as important structural elements". Biochemistry. 43 (12): 3628–36. doi:10.1021/bi0360004. PMID 15035633.
• Andrade J, Pearce ST, Zhao H, Barroso M (Dec 2004). "Interactions among p22, glyceraldehyde-3-phosphate dehydrogenase and microtubules". The Biochemical Journal. 384 (Pt 2): 327–36. doi:10.1042/BJ20040622. PMC 1134116. PMID 15312048.
• Ben Ammar Y, Takeda S, Sugawara M, Miyano M, Mori H, Wakabayashi S (Oct 2005). "Crystallization and preliminary crystallographic analysis of the human calcineurin homologous protein CHP2 bound to the cytoplasmic region of the Na+/H+ exchanger NHE1". Acta Crystallographica Section F. 61 (Pt 10): 956–8. doi:10.1107/S1744309105030836. PMC 1991313. PMID 16511206.
• Mishima M, Wakabayashi S, Kojima C (Jan 2007). "Solution structure of the cytoplasmic region of Na+/H+ exchanger 1 complexed with essential cofactor calcineurin B homologous protein 1". The Journal of Biological Chemistry. 282 (4): 2741–51. doi:10.1074/jbc.M604092200. PMID 17050540.

Vanjski linkovi

• Lokacija ljudskog genoma CHP1 i stranica sa detaljima o genu CHP1 u UCSC Genome Browseru.