C15orf15

Vjerovatni protein RLP24 ribosomske biogeneze je protein koji je kod ljudi kodiran genom RSL24D1.^[5][6][7] Patricia Hartz (2010) mapirala je gen RSL24D1 na hromosomu 15, sekvenca q21.3, na osnovu poravnanja sekvence (AF201949) sa genomskom sekvencom (GRCh37).^[8] Ovaj gen kodira protein koji ima nizak nivo sličnosti sekvence s ljudskim ribosomskim proteinom L24. Iako se ovaj gen u bazama podataka o sekvencama naziva RPL24, L30 i 60S ribosomski protein L30, razlikuje se od ljudskih gena koji su službeno nazvani RPL24 (koji je i sam naveden kao ribosomski protein L30) i RPL30. Funkcija ovog gena još nije poznata. Ovaj gen koristi alternativne poliadenilacijske signale.^[7]

C15orf15
Identifikatori
Aliasi	RSL24D1
Vanjski ID-jevi	OMIM: 613262 MGI: 2681840 HomoloGene: 9462 GeneCards: RSL24D1
Lokacija gena (čovjek) Hrom. Hromosom 15 (čovjek)[1] Bend 15q21.3 Početak 55,180,806 bp[1] Kraj 55,197,049 bp[1]
Lokacija gena (miš) Hrom. Hromosom 9 (miš)[2] Bend 9|9 D Početak 73,020,708 bp[2] Kraj 73,030,615 bp[2]
Ontologija gena Molekularna funkcija • structural constituent of ribosome • GO:0001948, GO:0016582 vezivanje za proteine • vezivanje sa RNK Ćelijska komponenta • cytosolic large ribosomal subunit • ribozom • Jedarce • intracellular anatomical structure • jedro Biološki proces • assembly of large subunit precursor of preribosome • Biosinteza bjelančevina • ribosomal large subunit assembly • ribosome biogenesis Izvori:Amigo / QuickGO
Ortolozi
Vrste	Čovjek	Miš
Entrez	51187	225215
Ensembl	ENSG00000137876	ENSMUSG00000032215
UniProt	Q9UHA3	Q99L28
RefSeq (mRNK)	NM_016304	NM_198609
RefSeq (bjelančevina)	NP_057388	NP_941011
Lokacija (UCSC)	Chr 15: 55.18 – 55.2 Mb	Chr 9: 73.02 – 73.03 Mb
PubMed pretraga	[3]	[4]
Wikipodaci
Pogledaj/uredi – čovjek Pogledaj/uredi – miš

Kloniranje i ekspresija

Traženjem ortologa kvasca Rlp24, Saveanu et al. (2003) identificirali su čovjeka RSL24D1, kojeg su nazvali RLP24.

Funkcija gena

Saveanu et al. (2003) otkrili su da je kvašćev Rlp24 bio uključen u biogenezu ranih ribonukleopartikula prije 60S. Rlp24 se pridružio čestici pre-60S u jedarcetu i održavao ovu povezanost nakon eksporta pre-60S u citoplazmu. Iscrpljivanje Rlp24 rezultiralo je nestabilnim kompleksom.^[9]

Aminokiselinska sekvenca

Dužina polipeptidnog lanca je 163 aminokiselina, a molekulska težina 19.621 Da ^[10]

1020304050 MRIEKCYFCSGPIYPGHGMMFVRNDCKVFRFCKSKCHKNFKKKRNPRKVR WTKAFRKAAGKELTVDNSFEFEKRRNEPIKYQRELWNKTIDAMKRVEEIK QKRQAKFIMNRLKKNKELQKVQDIKEVKQNIHLIRAPLAGKGKQLEEKMV QQLQEDVDMEDAP

Simboli

C: Cistein
D: Asparaginska kiselina
E: Glutaminska kiselina
F: Fenilalanin
G: Glicin
H: Histidin
I: Izoleucin
K: Lizin
L: Leucin
M: Metionin
N: Asparagin
P: Prolin
Q: Glutamin
R: Arginin
S: Serin
T: Treonin
V: Valin
W: Triptofan
Y: Tirozin

Reference

1. GRCh38: Ensembl release 89: ENSG00000137876 - Ensembl, maj 2017
2. GRCm38: Ensembl release 89: ENSMUSG00000032215 - Ensembl, maj 2017
3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
5. Saveanu C, Namane A, Gleizes PE, Lebreton A, Rousselle JC, Noaillac-Depeyre J, Gas N, Jacquier A, Fromont-Racine M (Jun 2003). "Sequential protein association with nascent 60S ribosomal particles". Mol Cell Biol. 23 (13): 4449–60. doi:10.1128/MCB.23.13.4449-4460.2003. PMC 164837. PMID 12808088.
6. Saveanu C, Bienvenu D, Namane A, Gleizes PE, Gas N, Jacquier A, Fromont-Racine M (Nov 2001). "Nog2p, a putative GTPase associated with pre-60S subunits and required for late 60S maturation steps". EMBO J. 20 (22): 6475–84. doi:10.1093/emboj/20.22.6475. PMC 125736. PMID 11707418.
7. "Entrez Gene: C15orf15 chromosome 15 open reading frame 15".
8. Hartz, P. A. Personal Communication. Baltimore, Md. 2/19/2010.
9. Saveanu, C., Namane, A., Gleizes, P.-E., Lebreton, A., Rousselle, J.-C., Noaillac-Depeyre, J., Gas, N., Jacquier, A., Fromont-Racine, M. Sequential protein association with nascent 60S ribosomal particles. Molec. Cell. Biol. 23: 4449-4460, 2003. PubMed: 12808088
10. "UniProt, Q9UHA3". Pristupljeno 12. 7. 2021.

Dopunska literatura

• Scherl A, Couté Y, Déon C, et al. (2003). "Functional proteomic analysis of human nucleolus". Mol. Biol. Cell. 13 (11): 4100–9. doi:10.1091/mbc.E02-05-0271. PMC 133617. PMID 12429849.
• Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932.
• Ota T, Suzuki Y, Nishikawa T, et al. (2004). "Complete sequencing and characterization of 21,243 full-length human cDNAs". Nat. Genet. 36 (1): 40–5. doi:10.1038/ng1285. PMID 14702039.
• Lehner B, Sanderson CM (2004). "A protein interaction framework for human mRNA degradation". Genome Res. 14 (7): 1315–23. doi:10.1101/gr.2122004. PMC 442147. PMID 15231747.
• Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC 528928. PMID 15489334.
• Andersen JS, Lam YW, Leung AK, et al. (2005). "Nucleolar proteome dynamics". Nature. 433 (7021): 77–83. Bibcode:2005Natur.433...77A. doi:10.1038/nature03207. PMID 15635413. S2CID 4344740.
• Rual JF, Venkatesan K, Hao T, et al. (2005). "Towards a proteome-scale map of the human protein-protein interaction network". Nature. 437 (7062): 1173–8. Bibcode:2005Natur.437.1173R. doi:10.1038/nature04209. PMID 16189514. S2CID 4427026.

Vanjski linkovi

• Lokacija ljudskog genoma RPL24 i stranica sa detaljima o genu RPL24 u UCSC Genome Browseru.
• Lokacija ljudskog genoma RSL24D1 i stranica sa detaljima o genu RSL24D1 u UCSC Genome Browseru.

Šablon:Ribosomskee podjedinice