Proteinski zatvarač makrofaga (CAPG), znan i kao aktinski regulatorni protein CAP-G, jest protein koji je kod ljudi kodiran genom CAPG sa hromosoma 2.[5][6][7]
Kratke činjenice Dostupne strukture, PDB ...
Zatvori
Dužina polipeptidnog lanca je 348 aminokiselina, a molkekulska iežina 38.499 Da.[8]
10 | | 20 | | 30 | | 40 | | 50 |
MYTAIPQSGS | | PFPGSVQDPG | | LHVWRVEKLK | | PVPVAQENQG | | VFFSGDSYLV |
LHNGPEEVSH | | LHLWIGQQSS | | RDEQGACAVL | | AVHLNTLLGE | | RPVQHREVQG |
NESDLFMSYF | | PRGLKYQEGG | | VESAFHKTST | | GAPAAIKKLY | | QVKGKKNIRA |
TERALNWDSF | | NTGDCFILDL | | GQNIFAWCGG | | KSNILERNKA | | RDLALAIRDS |
ERQGKAQVEI | | VTDGEEPAEM | | IQVLGPKPAL | | KEGNPEEDLT | | ADKANAQAAA |
LYKVSDATGQ | | MNLTKVADSS | | PFALELLISD | | DCFVLDNGLC | | GKIYIWKGRK |
ANEKERQAAL | | QVAEGFISRM | | QYAPNTQVEI | | LPQGHESPIF | | KQFFKDWK |
Ovaj gen kodira člana gelsolin/vilin porodice aktin-regulatornih proteina.[9] Kodirani protein reverzibilno blokira bodljikave krajeve F-aktinskih filamenata na Ca2+ski i fosfoinozitidno-regulisan način, ali ne prekida prethodno formirane aktinske filamente. Pokrivanjem bodljikavih krajeva aktinskih filamenata, kodirani protein doprinosi kontroli pokretljivosti zasnovanoj na aktinu u nemišićnim ćelijama. Uočene su alternativno prerađene varijanta transkripta, ali nisu u potpunosti opisane.[7]
- Southwick FS (1995). "Gain-of-function mutations conferring actin-severing activity to human macrophage cap G". J. Biol. Chem. 270 (1): 45–8. doi:10.1074/jbc.270.1.45. PMID 7814409.
- Mishra VS, Henske EP, Kwiatkowski DJ, Southwick FS (1995). "The human actin-regulatory protein cap G: gene structure and chromosome location". Genomics. 23 (3): 560–5. doi:10.1006/geno.1994.1543. PMID 7851883.
- Maruyama K, Sugano S (1994). "Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides". Gene. 138 (1–2): 171–4. doi:10.1016/0378-1119(94)90802-8. PMID 8125298.
- Hirano T, Kobayashi R, Hirano M (1997). "Condensins, chromosome condensation protein complexes containing XCAP-C, XCAP-E and a Xenopus homolog of the Drosophila Barren protein". Cell. 89 (4): 511–21. doi:10.1016/S0092-8674(00)80233-0. PMID 9160743. S2CID 15061740.
- Cabello OA, Eliseeva E, He WG, et al. (2002). "Cell cycle-dependent expression and nucleolar localization of hCAP-H". Mol. Biol. Cell. 12 (11): 3527–37. doi:10.1091/mbc.12.11.3527. PMC 60273. PMID 11694586.
- Van Impe K, De Corte V, Eichinger L, et al. (2003). "The Nucleo-cytoplasmic actin-binding protein CapG lacks a nuclear export sequence present in structurally related proteins". J. Biol. Chem. 278 (20): 17945–52. doi:10.1074/jbc.M209946200. PMID 12637565.
- De Corte V, Van Impe K, Bruyneel E, et al. (2005). "Increased importin-beta-dependent nuclear import of the actin modulating protein CapG promotes cell invasion". J. Cell Sci. 117 (Pt 22): 5283–92. doi:10.1242/jcs.01410. PMID 15454578.
- Watari A, Takaki K, Higashiyama S, et al. (2007). "Suppression of tumorigenicity, but not anchorage independence, of human cancer cells by new candidate tumor suppressor gene CapG". Oncogene. 25 (56): 7373–80. doi:10.1038/sj.onc.1209732. PMID 16767159.
- Chi A, Valencia JC, Hu ZZ, et al. (2007). "Proteomic and bioinformatic characterization of the biogenesis and function of melanosomes". J. Proteome Res. 5 (11): 3135–44. doi:10.1021/pr060363j. PMID 17081065.