YBX1

Y-kutijski vezujući protein 1 poznat i kao Y-kutijski transkripcijski faktor ili nukleazno osjetljivi element-vezujući protein 1 jest protein koji je kod ljudi kodiran genom YBX1 sa hromosoma 1.^[5]

YBX1
Dostupne strukture PDB Pretraga ortologa: PDBe RCSB Spisak PDB ID kodova 1H95
Identifikatori
Aliasi	YBX1
Vanjski ID-jevi	OMIM: 154030 MGI: 99146 HomoloGene: 88707 GeneCards: YBX1
Lokacija gena (čovjek) Hrom. Hromosom 1 (čovjek)[1] Bend 1p34.2 Početak 42,682,418 bp[1] Kraj 42,703,805 bp[1]
Lokacija gena (miš) Hrom. Hromosom 4 (miš)[2] Bend 4|4 D2.1 Početak 119,135,178 bp[2] Kraj 119,151,801 bp[2]
Ontologija gena Molekularna funkcija • vezivanje sa DNK • GO:0001131, GO:0001151, GO:0001130, GO:0001204 DNA-binding transcription factor activity • GO:0001077, GO:0001212, GO:0001213, GO:0001211, GO:0001205 DNA-binding transcription activator activity, RNA polymerase II-specific • GO:0000980 RNA polymerase II cis-regulatory region sequence-specific DNA binding • GO:0001948, GO:0016582 vezivanje za proteine • vezivanje sa RNK • nucleic acid binding • GTPase binding • double-stranded DNA binding • single-stranded DNA binding • chromatin binding • GO:0001200, GO:0001133, GO:0001201 DNA-binding transcription factor activity, RNA polymerase II-specific Ćelijska komponenta • citoplazma • CRD-mediated mRNA stability complex • Jedarna membrana • intracellular membrane-bounded organelle • U12-type spliceosomal complex • nukleoplazma • extracellular region • cytoplasmic stress granule • Egzosom • jedro • histone pre-mRNA 3'end processing complex • citosol • ribonukleoprotein Biološki proces • CRD-mediated mRNA stabilization • mRNA splicing, via spliceosome • GO:0009373 regulation of transcription, DNA-templated • mRNA processing • transcription by RNA polymerase II • protein localization to cytoplasmic stress granule • transcription, DNA-templated • positive regulation of cell division • GO:0003257, GO:0010735, GO:1901228, GO:1900622, GO:1904488 positive regulation of transcription by RNA polymerase II • in utero embryonic development • Prerada RNK • negative regulation of striated muscle cell differentiation • GO:1901227 negative regulation of transcription by RNA polymerase II • Notch signaling pathway • cellular response to interleukin-7 Izvori:Amigo / QuickGO
Ortolozi
Vrste	Čovjek	Miš
Entrez	4904	22608
Ensembl	ENSG00000065978	ENSMUSG00000028639
UniProt	P67809	P62960
RefSeq (mRNK)	NM_004559	NM_011732
RefSeq (bjelančevina)	NP_004550	NP_035862
Lokacija (UCSC)	Chr 1: 42.68 – 42.7 Mb	Chr 4: 119.14 – 119.15 Mb
PubMed pretraga	[3]	[4]
Wikipodaci
Pogledaj/uredi – čovjek Pogledaj/uredi – miš

Aminokiselinska sekvenca

Dužina polipeptidnog lanca je 324 aminokiseline, a molekulska težina 35.924 Da.^[6]

• C: Cistein
• D: Asparaginska kiselina
• E: Glutaminska kiselina
• F: Fenilalanin
• G: Glicin
• H: Histidin
• I: Izoleucin
• K: Lizin
• L: Leucin
• M: Metionin
• N: Asparagin
• P: Prolin
• Q: Glutamin
• R: Arginin
• S: Serin
• T: Treonin
• V: Valin
• W: Triptofan
• Y: Tirozin

10		20		30		40		50
MSSEAETQQP		PAAPPAAPAL		SAADTKPGTT		GSGAGSGGPG		GLTSAAPAGG
DKKVIATKVL		GTVKWFNVRN		GYGFINRNDT		KEDVFVHQTA		IKKNNPRKYL
RSVGDGETVE		FDVVEGEKGA		EAANVTGPGG		VPVQGSKYAA		DRNHYRRYPR
RRGPPRNYQQ		NYQNSESGEK		NEGSESAPEG		QAQQRRPYRR		RRFPPYYMRR
PYGRRPQYSN		PPVQGEVMEG		ADNQGAGEQG		RPVRQNMYRG		YRPRFRRGPP
RQRQPREDGN		EEDKENQGDE		TQGQQPPQRR		YRRNFNYRRR		RPENPKPQDG
KETKAADPPA		ENSSAPEAEQ		GGAE

Klinički značaj

YBX1 je potencijalna meta lijeka u terapiji raka.^[7] YB-1 pomaže u replikaciji adenovirusa tipa 5, vektora koji se često koristi u genskoj terapiji.^[7] Dakle, YB-1 može izazvati "onkolitski" efekt u YB-1 pozitivnim ćelijama raka tretiranim adenovirusima.

Interakcije

Pokazalo se da protein 1 koji vezuje Y kutiju reaguje sa:

• ANKRD2,^[8]
• CTCF,^[9]
• P53,^[8][10]
• PCNA,^[11]
• RBBP6,^[12] i
• SFRS9.^[13]

Reference

1. GRCh38: Ensembl release 89: ENSG00000065978 - Ensembl, maj 2017
2. GRCm38: Ensembl release 89: ENSMUSG00000028639 - Ensembl, maj 2017
3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
5. "Entrez Gene: YBX1 Y box binding protein 1".
6. "UniProt, P67809" (jezik: eng.). Pristupljeno 28. 11. 2021.CS1 održavanje: nepoznati jezik (link)
7. Lage H, Surowiak P, Holm PS (novembar 2008). "[YB-1 as a potential target in cancer therapy]". Pathologe (jezik: njemački). 29 Suppl 2: 187–90. doi:10.1007/s00292-008-1030-2. PMID 18773210.
8. Kojic S, Medeot E, Guccione E, Krmac H, Zara I, Martinelli V, Valle G, Faulkner G (2004). "The Ankrd2 protein, a link between the sarcomere and the nucleus in skeletal muscle". J. Mol. Biol. 339 (2): 313–25. doi:10.1016/j.jmb.2004.03.071. PMID 15136035.
9. Chernukhin IV, Shamsuddin S, Robinson AF, Carne AF, Paul A, El-Kady AI, Lobanenkov VV, Klenova EM (2000). "Physical and functional interaction between two pluripotent proteins, the Y-box DNA/RNA-binding factor, YB-1, and the multivalent zinc finger factor, CTCF". J. Biol. Chem. 275 (38): 29915–21. doi:10.1074/jbc.M001538200. PMID 10906122.
10. Okamoto T, Izumi H, Imamura T, Takano H, Ise T, Uchiumi T, Kuwano M, Kohno K (2000). "Direct interaction of p53 with the Y-box binding protein, YB-1: a mechanism for regulation of human gene expression". Oncogene. 19 (54): 6194–202. doi:10.1038/sj.onc.1204029. PMID 11175333.
11. Ise T, Nagatani G, Imamura T, Kato K, Takano H, Nomoto M, Izumi H, Ohmori H, Okamoto T, Ohga T, Uchiumi T, Kuwano M, Kohno K (1999). "Transcription factor Y-box binding protein 1 binds preferentially to cisplatin-modified DNA and interacts with proliferating cell nuclear antigen". Cancer Res. 59 (2): 342–6. PMID 9927044.
12. Chibi M, Meyer M, Skepu A, G Rees DJ, Moolman-Smook JC, Pugh DJ (2008). "RBBP6 interacts with multifunctional protein YB-1 through its RING finger domain, leading to ubiquitination and proteosomal degradation of YB-1". J. Mol. Biol. 384 (4): 908–16. doi:10.1016/j.jmb.2008.09.060. PMID 18851979.
13. Raffetseder U, Frye B, Rauen T, Jürchott K, Royer HD, Jansen PL, Mertens PR (2003). "Splicing factor SRp30c interaction with Y-box protein-1 confers nuclear YB-1 shuttling and alternative splice site selection". J. Biol. Chem. 278 (20): 18241–8. doi:10.1074/jbc.M212518200. PMID 12604611.

Dopunska literatura

• Kolluri R, Torrey TA, Kinniburgh AJ (1992). "A CT promoter element binding protein: definition of a double-strand and a novel single-strand DNA binding motif". Nucleic Acids Res. 20 (1): 111–6. doi:10.1093/nar/20.1.111. PMC 5356827. PMID 1738588.
• Kolluri R, Kinniburgh AJ (1991). "Full length cDNA sequence encoding a nuclease-sensitive element DNA binding protein". Nucleic Acids Res. 19 (17): 4771. doi:10.1093/nar/19.17.4771. PMC 328725. PMID 1891370.
• Sakura H, Maekawa T, Imamoto F, et al. (1989). "Two human genes isolated by a novel method encode DNA-binding proteins containing a common region of homology". Gene. 73 (2): 499–507. doi:10.1016/0378-1119(88)90514-8. PMID 2977358.
• Didier DK, Schiffenbauer J, Woulfe SL, et al. (1988). "Characterization of the cDNA encoding a protein binding to the major histocompatibility complex class II Y box". Proc. Natl. Acad. Sci. U.S.A. 85 (19): 7322–6. Bibcode:1988PNAS...85.7322D. doi:10.1073/pnas.85.19.7322. PMC 282178. PMID 3174636.
• Kudo S, Mattei MG, Fukuda M (1995). "Characterization of the gene for dbpA, a family member of the nucleic-acid-binding proteins containing a cold-shock domain". Eur. J. Biochem. 231 (1): 72–82. doi:10.1111/j.1432-1033.1995.tb20672.x. PMID 7628487.
• Lloberas J, Maki RA, Celada A (1995). "Repression of major histocompatibility complex I-A beta gene expression by dbpA and dbpB (mYB-1) proteins". Mol. Cell. Biol. 15 (9): 5092–9. doi:10.1128/mcb.15.9.5092. PMC 230756. PMID 7651426.
• Chen NN, Chang CF, Gallia GL, et al. (1995). "Cooperative action of cellular proteins YB-1 and Pur alpha with the tumor antigen of the human JC polyomavirus determines their interaction with the viral lytic control element". Proc. Natl. Acad. Sci. U.S.A. 92 (4): 1087–91. Bibcode:1995PNAS...92.1087C. doi:10.1073/pnas.92.4.1087. PMC 42642. PMID 7862639.
• Horwitz EM, Maloney KA, Ley TJ (1994). "A human protein containing a "cold shock" domain binds specifically to H-DNA upstream from the human gamma-globin genes". J. Biol. Chem. 269 (19): 14130–9. doi:10.1016/S0021-9258(17)36764-9. PMID 8188694.
• Kashanchi F, Duvall JF, Dittmer J, et al. (1994). "Involvement of transcription factor YB-1 in human T-cell lymphotropic virus type I basal gene expression". J. Virol. 68 (1): 561–5. doi:10.1128/JVI.68.1.561-565.1994. PMC 236322. PMID 8254772.
• Makino Y, Ohga T, Toh S, et al. (1996). "Structural and functional analysis of the human Y-box binding protein (YB-1) gene promoter". Nucleic Acids Res. 24 (10): 1873–8. doi:10.1093/nar/24.10.1873. PMC 145870. PMID 8657568.
• Funke B, Zuleger B, Benavente R, et al. (1996). "The mouse poly(C)-binding protein exists in multiple isoforms and interacts with several RNA-binding proteins". Nucleic Acids Res. 24 (19): 3821–8. doi:10.1093/nar/24.19.3821. PMC 146158. PMID 8871564.
• Koike K, Uchiumi T, Ohga T, et al. (1998). "Nuclear translocation of the Y-box binding protein by ultraviolet irradiation". FEBS Lett. 417 (3): 390–4. doi:10.1016/S0014-5793(97)01296-9. PMID 9409758. S2CID 24656107.
• Toh S, Nakamura T, Ohga T, et al. (1998). "Genomic organization of the human Y-box protein (YB-1) gene". Gene. 206 (1): 93–7. doi:10.1016/S0378-1119(97)00570-2. PMID 9461420.
• Sawaya BE, Khalili K, Amini S (1998). "Transcription of the human immunodeficiency virus type 1 (HIV-1) promoter in central nervous system cells: effect of YB-1 on expression of the HIV-1 long terminal repeat". J. Gen. Virol. 79. ( Pt 2) (2): 239–46. doi:10.1099/0022-1317-79-2-239. PMID 9472608.
• Mertens PR, Alfonso-Jaume MA, Steinmann K, Lovett DH (1999). "A synergistic interaction of transcription factors AP2 and YB-1 regulates gelatinase A enhancer-dependent transcription". J. Biol. Chem. 273 (49): 32957–65. doi:10.1074/jbc.273.49.32957. PMID 9830047.
• Ise T, Nagatani G, Imamura T, et al. (1999). "Transcription factor Y-box binding protein 1 binds preferentially to cisplatin-modified DNA and interacts with proliferating cell nuclear antigen". Cancer Res. 59 (2): 342–6. PMID 9927044.
• Safak M, Gallia GL, Khalili K (1999). "Reciprocal interaction between two cellular proteins, Puralpha and YB-1, modulates transcriptional activity of JCVCY in glial cells". Mol. Cell. Biol. 19 (4): 2712–23. doi:10.1128/mcb.19.4.2712. PMC 84064. PMID 10082537.
• Kelm RJ, Cogan JG, Elder PK, et al. (1999). "Molecular interactions between single-stranded DNA-binding proteins associated with an essential MCAT element in the mouse smooth muscle alpha-actin promoter". J. Biol. Chem. 274 (20): 14238–45. doi:10.1074/jbc.274.20.14238. PMID 10318844.
• Safak M, Gallia GL, Ansari SA, Khalili K (1999). "Physical and functional interaction between the Y-box binding protein YB-1 and human polyomavirus JC virus large T antigen". J. Virol. 73 (12): 10146–57. doi:10.1128/JVI.73.12.10146-10157.1999. PMC 113067. PMID 10559330.
• Ansari SA, Safak M, Gallia GL, et al. (1999). "Interaction of YB-1 with human immunodeficiency virus type 1 Tat and TAR RNA modulates viral promoter activity". J. Gen. Virol. 80. ( Pt 10) (10): 2629–38. doi:10.1099/0022-1317-80-10-2629. PMID 10573156.
• Basaki Y, Hosoi F, Oda Y, et al. (2007). "Akt-dependent nuclear localization of Y-box-binding protein 1 in acquisition of malignant characteristics by human ovarian cancer cells". Oncogene. 26 (19): 2736–46. doi:10.1038/sj.onc.1210084. PMID 17072343.

Vanjski linkovi

• YBX1 protein, human na US National Library of Medicine Medical Subject Headings (MeSH)

Ovaj članak uključuje tekst iz Nacionalne medicinske biblioteke Sjedinjenih Država, koji je u javnom vlasništvu.