STX6

Sintaksin-6 jest protein koji je kod ljudi kodiran genom STX6 sa hromosoma 1.^[5][6]

STX6
Dostupne strukture PDB Pretraga ortologa: PDBe RCSB Spisak PDB ID kodova 2NPS, 4J2C
Identifikatori
Aliasi	STX6
Vanjski ID-jevi	OMIM: 603944 MGI: 1926235 HomoloGene: 115622 GeneCards: STX6
Lokacija gena (čovjek) Hrom. Hromosom 1 (čovjek)[1] Bend 1q25.3 Početak 180,972,712 bp[1] Kraj 181,023,121 bp[1]
Lokacija gena (miš) Hrom. Hromosom 1 (miš)[2] Bend 1|1 G3 Početak 155,034,461 bp[2] Kraj 155,084,002 bp[2]
Ontologija gena Molekularna funkcija • GO:0001948, GO:0016582 vezivanje za proteine • syntaxin binding • SNAP receptor activity • SNARE binding Ćelijska komponenta • membrana • ćelijska membrana • SNARE complex • early endosome • phagocytic vesicle • perinuklearno područje citoplazme • clathrin-coated vesicle • trans-Golđijeva mreža • integral component of membrane • Golđijeva membrana • trans-Golgi network membrane • Golđijev aparat • nukleoplazma • citosol • Sinapsna vezikula • integral component of synaptic vesicle membrane • Endomembranski sistem Biološki proces • endosome organization • regulation of protein localization • vesicle docking • retrograde transport, endosome to Golgi • Golgi vesicle transport • protein transport • Fuzija vezikula • Golgi ribbon formation • vesicle-mediated transport • intracellular protein transport • endocytic recycling • synaptic vesicle to endosome fusion Izvori:Amigo / QuickGO
Ortolozi
Vrste	Čovjek	Miš
Entrez	10228	58244
Ensembl	ENSG00000135823	ENSMUSG00000026470
UniProt	O43752	Q9JKK1
RefSeq (mRNK)	NM_001286210 NM_005819	NM_021433
RefSeq (bjelančevina)	NP_001273139 NP_005810	NP_067408
Lokacija (UCSC)	Chr 1: 180.97 – 181.02 Mb	Chr 1: 155.03 – 155.08 Mb
PubMed pretraga	[3]	[4]
Wikipodaci
Pogledaj/uredi – čovjek Pogledaj/uredi – miš

Aminokiselinska sekvenca

Dužina polipeptidnog lanca je 255 aminokiselina, a molekulska težina 29.176 Da.

• C: Cistein
• D: Asparaginska kiselina
• E: Glutaminska kiselina
• F: Fenilalanin
• G: Glicin
• H: Histidin
• I: Izoleucin
• K: Lizin
• L: Leucin
• M: Metionin
• N: Asparagin
• P: Prolin
• Q: Glutamin
• R: Arginin
• S: Serin
• T: Treonin
• V: Valin
• W: Triptofan
• Y: Tirozin

10		20		30		40		50
MSMEDPFFVV		KGEVQKAVNT		AQGLFQRWTE		LLQDPSTATR		EEIDWTTNEL
RNNLRSIEWD		LEDLDETISI		VEANPRKFNL		DATELSIRKA		FITSTRQVVR
DMKDQMSTSS		VQALAERKNR		QALLGDSGSQ		NWSTGTTDKY		GRLDRELQRA
NSHFIEEQQA		QQQLIVEQQD		EQLELVSGSI		GVLKNMSQRI		GGELEEQAVM
LEDFSHELES		TQSRLDNVMK		KLAKVSHMTS		DRRQWCAIAI		LFAVLLVVLI
LFLVL

Struktura

Članovi ove grupe proteina, koji se nalaze u N-terminalu različitih SNARE proteina, imaju strukturu koja se sastoji od antiparalelnog snopa sa tri spirale. Njihova tačna funkcija nije utvrđena, iako je poznato da oni regulišu SNARE motiv, kao i da posreduju u različitim interakcijama protein-protein uključenim u membranski transport.^[7]

Funkcija

SNARE igraju vitalnu ulogu u prometu ćelijskoh tereta. Vezikule se spajaju sa ćelijskom membranom, uz pomoć SNARE proteina. SNARE motivi formiraju četveroheliksni snop koji doprinosi fuziji dvije membrane. Tačnije, N-terminalni domen vezuje se na SNARE motiv, a ova unutarmolekulska interakcija smanjuje stopu povezivanja sa partnerom SNARE. Međutim, funkcija domena N terminala još uvijek nije u potpunosti razjašnjena.^[7]

N-terminalni proteinski domen

Proteinski domen sintaksin-6 N-terminalni proteinski domen je rastvorljivi receptor proteina vezivanja faktora osetljivog na N-etilmaleimid (SNARE) koji se nalazi u endosomnom transportu vezikula. Dio je porodice ciljnih SNARE (t-SNARE). To je vitalna pomoć za unos i eksport ćelijskog tereta u procesu zvanom ćelijski promet. Njegov SNARE motiv pokazuje značajnu homologiju za sintaksinom 1A i S25C, što ukazuje na sličnost putem evolucijsko konzerviranje. N-terminalna struktura domena sintaksina-6 pokazuje snažnu strukturnu sličnost sa N-terminalnim domenima sintaksina 1A, Sso1p i Vam3p; uprkos veoma niskom nivou sličnosti sekvenci. SNARE u suštini funkcioniše kao privez za držanje vezikule. Citoplazmatski regioni SNARE koji se nalaze na transportnim vezikulama i ciljnim membranama djeluju međusobno, a zatim se formira zavojnica sa četiri heliksa. Ovo povezuje ćelijsku membranu i vezikule zajedno na takav način da prevazilazi energetsku barijeru za spajanje dva lipidna dvosloja. Ovo je način na koji se razmenjuje ćelijski teret. Ovaj konkretan unos fokusira se na N-terminalni domen sintaksina 6.^[8]

Interakcije

Pokazalo se da STX6 ima interakcije sa SNAP23,^[9] VAMP3^[10] i VAMP4.^[10]

Reference

1. GRCh38: Ensembl release 89: ENSG00000135823 - Ensembl, maj 2017
2. GRCm38: Ensembl release 89: ENSMUSG00000026470 - Ensembl, maj 2017
3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
5. Martín-Martín B, Nabokina SM, Lazo PA, Mollinedo F (March 1999). "Co-expression of several human syntaxin genes in neutrophils and differentiating HL-60 cells: variant isoforms and detection of syntaxin 1" (PDF). Journal of Leukocyte Biology. 65 (3): 397–406. doi:10.1002/jlb.65.3.397. hdl:10261/59829. PMID 10080545. S2CID 18988377.
6. "Entrez Gene: STX6 syntaxin 6".
7. Misura KM, Bock JB, Gonzalez LC, Scheller RH, Weis WI (July 2002). "Three-dimensional structure of the amino-terminal domain of syntaxin 6, a SNAP-25 C homolog". Proceedings of the National Academy of Sciences of the United States of America. 99 (14): 9184–9. doi:10.1073/pnas.132274599. PMC 123115. PMID 12082176.
8. Jung JJ, Inamdar SM, Tiwari A, Choudhury A (August 2012). "Regulation of intracellular membrane trafficking and cell dynamics by syntaxin-6". Bioscience Reports. 32 (4): 383–91. doi:10.1042/BSR20120006. PMC 3392101. PMID 22489884.
9. Martín-Martín B, Nabokina SM, Blasi J, Lazo PA, Mollinedo F (October 2000). "Involvement of SNAP-23 and syntaxin 6 in human neutrophil exocytosis". Blood. 96 (7): 2574–83. doi:10.1182/blood.V96.7.2574. PMID 11001914.
10. Mallard F, Tang BL, Galli T, Tenza D, Saint-Pol A, Yue X, et al. (February 2002). "Early/recycling endosomes-to-TGN transport involves two SNARE complexes and a Rab6 isoform". The Journal of Cell Biology. 156 (4): 653–64. doi:10.1083/jcb.200110081. PMC 2174079. PMID 11839770.

Dopunska literatura

• Andersson B, Wentland MA, Ricafrente JY, Liu W, Gibbs RA (April 1996). "A "double adaptor" method for improved shotgun library construction". Analytical Biochemistry. 236 (1): 107–13. doi:10.1006/abio.1996.0138. PMID 8619474.
• Bock JB, Lin RC, Scheller RH (July 1996). "A new syntaxin family member implicated in targeting of intracellular transport vesicles". The Journal of Biological Chemistry. 271 (30): 17961–5. doi:10.1074/jbc.271.30.17961. PMID 8663448.
• Yu W, Andersson B, Worley KC, Muzny DM, Ding Y, Liu W, et al. (April 1997). "Large-scale concatenation cDNA sequencing". Genome Research. 7 (4): 353–8. doi:10.1101/gr.7.4.353. PMC 139146. PMID 9110174.
• Simonsen A, Lippé R, Christoforidis S, Gaullier JM, Brech A, Callaghan J, et al. (July 1998). "EEA1 links PI(3)K function to Rab5 regulation of endosome fusion". Nature. 394 (6692): 494–8. doi:10.1038/28879. PMID 9697774. S2CID 4305220.
• Wong SH, Xu Y, Zhang T, Griffiths G, Lowe SL, Subramaniam VN, et al. (January 1999). "GS32, a novel Golgi SNARE of 32 kDa, interacts preferentially with syntaxin 6". Molecular Biology of the Cell. 10 (1): 119–34. doi:10.1091/mbc.10.1.119. PMC 25158. PMID 9880331.
• Steegmaier M, Klumperman J, Foletti DL, Yoo JS, Scheller RH (June 1999). "Vesicle-associated membrane protein 4 is implicated in trans-Golgi network vesicle trafficking". Molecular Biology of the Cell. 10 (6): 1957–72. doi:10.1091/mbc.10.6.1957. PMC 25394. PMID 10359608.
• Simonsen A, Gaullier JM, D'Arrigo A, Stenmark H (October 1999). "The Rab5 effector EEA1 interacts directly with syntaxin-6". The Journal of Biological Chemistry. 274 (41): 28857–60. doi:10.1074/jbc.274.41.28857. PMID 10506127.
• Scales SJ, Chen YA, Yoo BY, Patel SM, Doung YC, Scheller RH (May 2000). "SNAREs contribute to the specificity of membrane fusion". Neuron. 26 (2): 457–64. doi:10.1016/S0896-6273(00)81177-0. PMID 10839363.
• Martín-Martín B, Nabokina SM, Blasi J, Lazo PA, Mollinedo F (October 2000). "Involvement of SNAP-23 and syntaxin 6 in human neutrophil exocytosis". Blood. 96 (7): 2574–83. doi:10.1182/blood.V96.7.2574. PMID 11001914.
• Wade N, Bryant NJ, Connolly LM, Simpson RJ, Luzio JP, Piper RC, James DE (June 2001). "Syntaxin 7 complexes with mouse Vps10p tail interactor 1b, syntaxin 6, vesicle-associated membrane protein (VAMP)8, and VAMP7 in b16 melanoma cells" (PDF). The Journal of Biological Chemistry. 276 (23): 19820–7. doi:10.1074/jbc.M010838200. PMID 11278762. S2CID 38183181.
• Charest A, Lane K, McMahon K, Housman DE (August 2001). "Association of a novel PDZ domain-containing peripheral Golgi protein with the Q-SNARE (Q-soluble N-ethylmaleimide-sensitive fusion protein (NSF) attachment protein receptor) protein syntaxin 6". The Journal of Biological Chemistry. 276 (31): 29456–65. doi:10.1074/jbc.M104137200. PMID 11384996.
• Rotem-Yehudar R, Galperin E, Horowitz M (August 2001). "Association of insulin-like growth factor 1 receptor with EHD1 and SNAP29". The Journal of Biological Chemistry. 276 (35): 33054–60. doi:10.1074/jbc.M009913200. PMID 11423532.
• Cheng J, Moyer BD, Milewski M, Loffing J, Ikeda M, Mickle JE, et al. (February 2002). "A Golgi-associated PDZ domain protein modulates cystic fibrosis transmembrane regulator plasma membrane expression". The Journal of Biological Chemistry. 277 (5): 3520–9. doi:10.1074/jbc.M110177200. PMID 11707463.
• Mallard F, Tang BL, Galli T, Tenza D, Saint-Pol A, Yue X, et al. (February 2002). "Early/recycling endosomes-to-TGN transport involves two SNARE complexes and a Rab6 isoform". The Journal of Cell Biology. 156 (4): 653–64. doi:10.1083/jcb.200110081. PMC 2174079. PMID 11839770.
• Martinez-Arca S, Rudge R, Vacca M, Raposo G, Camonis J, Proux-Gillardeaux V, et al. (July 2003). "A dual mechanism controlling the localization and function of exocytic v-SNAREs". Proceedings of the National Academy of Sciences of the United States of America. 100 (15): 9011–6. doi:10.1073/pnas.1431910100. PMC 166429. PMID 12853575.
• Kim BY, Ueda M, Kominami E, Akagawa K, Kohsaka S, Akazawa C (November 2003). "Identification of mouse Vps16 and biochemical characterization of mammalian class C Vps complex". Biochemical and Biophysical Research Communications. 311 (3): 577–82. doi:10.1016/j.bbrc.2003.10.030. PMID 14623309.
• Huynh H, Bottini N, Williams S, Cherepanov V, Musumeci L, Saito K, et al. (September 2004). "Control of vesicle fusion by a tyrosine phosphatase". Nature Cell Biology. 6 (9): 831–9. doi:10.1038/ncb1164. PMID 15322554. S2CID 25311826.

Vanjski linkovi

• STX6 lokacija ljudskog genoma UCSC Genome Browser.

STX6 detalji ljudskog genoma u UCSC Genome Browser.