SFRS14

Pretpostavljeni faktor prerade RNK, arginin/serinom bogati protein 14 je protein koji je kod ljudi kodiran genom SFRS14.^[5][6]

SFRS14
Dostupne strukture PDB Pretraga ortologa: PDBe RCSB Spisak PDB ID kodova 1X4P
Identifikatori
Aliasi	SUGP2
Vanjski ID-jevi	OMIM: 607993 MGI: 2678085 HomoloGene: 8923 GeneCards: SUGP2
Lokacija gena (čovjek) Hrom. Hromosom 19 (čovjek)[1] Bend 19p13.11 Početak 18,990,888 bp[1] Kraj 19,034,023 bp[1]
Lokacija gena (miš) Hrom. Hromosom 8 (miš)[2] Bend 8|8 B3.3 Početak 70,234,226 bp[2] Kraj 70,279,915 bp[2]
Obrazac RNK ekspresije Više referentnih podataka o ekspresiji
Ontologija gena Molekularna funkcija • nucleic acid binding • vezivanje sa RNK Ćelijska komponenta • jedro • nukleoplazma • nuclear body Biološki proces • mRNA processing • GO:0033168, GO:0036404, GO:1902360 RNA processing • Prerada RNK Izvori:Amigo / QuickGO
Ortolozi
Vrste	Čovjek	Miš
Entrez	10147	234373
Ensembl	ENSG00000064607	ENSMUSG00000036054
UniProt	Q8IX01	Q8CH09
RefSeq (mRNK)	NM_001017392 NM_014884 NM_001321697 NM_001321698 NM_001321699 NM_001352071	NM_001168290 NM_172755
RefSeq (bjelančevina)	NP_001017392 NP_001308626 NP_001308627 NP_001308628 NP_055699 NP_001339000	NP_001161762 NP_766343
Lokacija (UCSC)	Chr 19: 18.99 – 19.03 Mb	Chr 8: 70.23 – 70.28 Mb
PubMed pretraga	[3]	[4]
Wikipodaci
Pogledaj/uredi – čovjek Pogledaj/uredi – miš

Aminokiselinska sekvenca

Simboli

C: Cistein
D: Asparaginska kiselina
E: Glutaminska kiselina
F: Fenilalanin
G: Glicin
H: Histidin
I: Izoleucin
K: Lizin
L: Leucin
M: Metionin
N: Asparagin
P: Prolin
Q: Glutamin
R: Arginin
S: Serin
T: Treonin
V: Valin
W: Triptofan
Y: Tirozin

10		20		30		40		50
MAARRITQET		FDAVLQEKAK		RYHMDASGEA		VSETLQFKAQ		DLLRAVPRSR
AEMYDDVHSD		GRYSLSGSVA		HSRDAGREGL		RSDVFPGPSF		RSSNPSISDD
SYFRKECGRD		LEFSHSDSRD		QVIGHRKLGH		FRSQDWKFAL		RGSWEQDFGH
PVSQESSWSQ		EYSFGPSAVL		GDFGSSRLIE		KECLEKESRD		YDVDHPGEAD
SVLRGGSQVQ		ARGRALNIVD		QEGSLLGKGE		TQGLLTAKGG		VGKLVTLRNV
STKKIPTVNR		ITPKTQGTNQ		IQKNTPSPDV		TLGTNPGTED		IQFPIQKIPL
GLDLKNLRLP		RRKMSFDIID		KSDVFSRFGI		EIIKWAGFHT		IKDDIKFSQL
FQTLFELETE		TCAKMLASFK		CSLKPEHRDF		CFFTIKFLKH		SALKTPRVDN
EFLNMLLDKG		AVKTKNCFFE		IIKPFDKYIM		RLQDRLLKSV		TPLLMACNAY
ELSVKMKTLS		NPLDLALALE		TTNSLCRKSL		ALLGQTFSLA		SSFRQEKILE
AVGLQDIAPS		PAAFPNFEDS		TLFGREYIDH		LKAWLVSSGC		PLQVKKAEPE
PMREEEKMIP		PTKPEIQAKA		PSSLSDAVPQ		RADHRVVGTI		DQLVKRVIEG
SLSPKERTLL		KEDPAYWFLS		DENSLEYKYY		KLKLAEMQRM		SENLRGADQK
PTSADCAVRA		MLYSRAVRNL		KKKLLPWQRR		GLLRAQGLRG		WKARRATTGT
QTLLSSGTRL		KHHGRQAPGL		SQAKPSLPDR		NDAAKDCPPD		PVGPSPQDPS
LEASGPSPKP		AGVDISEAPQ		TSSPCPSADI		DMKTMETAEK		LARFVAQVGP
EIEQFSIENS		TDNPDLWFLH		DQNSSAFKFY		RKKVFELCPS		ICFTSSPHNL
HTGGGDTTGS		QESPVDLMEG		EAEFEDEPPP		REAELESPEV		MPEEEDEDDE
DGGEEAPAPG		GAGKSEGSTP		ADGLPGEAAE		DDLAGAPALS		QASSGTCFPR
KRISSKSLKV		GMIPAPKRVC		LIQEPKVHEP		VRIAYDRPRG		RPMSKKKKPK
DLDFAQQKLT		DKNLGFQMLQ		KMGWKEGHGL		GSLGKGIREP		VSVGTPSEGE
GLGADGQEHK		EDTFDVFRQR		MMQMYRHKRA		NK

Kloniranje i ekspresija

Sekvenciranjem nasumično odabranih cDNK, koje odgovaraju relativno dugim transkriptima iz ljudskog mozga, Nagase et al. (1997) identificirali su djelimičnu SFRS14 cDNK, koju su nazvali KIAA0365.

Pretragom baze podataka EST i analizom genomskih sekvenci, Sampson i Hewitt (2003) identificirali su SFRS14 u punoj dužini, kao i povezanog člana porodice SURP, SF4 (SUGP1). SFRS14 kodira izvedeni protein od 1,083 aminokiseline, s procijenjenom molekulskom masom od 120,2 kD. I SF4 i SFRS14 sadrže 2 SURP motiv i jedna domen G-plohe na svojim C-krajevima. SFRS14 također sadrži N-kraj bogat argininima /serinima, što sugerira da je novi član porodice pre-iRNK faktora prerade povezanih sa SR.

Sampson i Hewitt (2003) također su klonirali mišje ortologe SF4 i SFRS14. Mišji protein Sfrs14 ima identičnu organizaciju domena i 73% identiteta sekvence sa svojim ljudskim homologom. Northern blot analiza otkrila je ekspresiju otprilike 3,5 kb SFRS14 transkripta samo u sjemenicima i fetusnom mozgu i bubrezima. RT-PCR identificirao je ekspresiju SFRS14 u skeletnim mišićima i mozgu odraslih.

Funkcija gena

Na temelju prisutnosti domena SURP, G-plohe i RS, Sampson i Hewitt (2003) sugeriraju da SFRS14 funkcionira u mehanizmima prerade preiRNK.

Struktura gena

Sampson i Hewitt (2003) utvrdili su da gen SFRS14 sadrži 12 egzona i da se proteže na 41,2 kb. SFRS14 podvrgava se [[alternativna prerada RNK|alternativnoj 3-osnovnoj preradi. Najvećem SFRS14 transkriptu nedostaje egzon 11, koji čini prva 103 bazna para UTR-a s tri prajmera.

Mapiranje

Hibridnom analizom zračenja Nagase et al. (1997) mapirali su gen SFRS14 na hromosomu 19. Analizom sekvence, Sampson i Hewitt (2003) mapirali su gene SFRS14 i SF4 unutar 300 kb, jedan od drugog, na hromosomu 19, pozicija na kratkom kraku: p13.11.^[7][8]

Reference

1. GRCh38: Ensembl release 89: ENSG00000064607 - Ensembl, maj 2017
2. GRCm38: Ensembl release 89: ENSMUSG00000036054 - Ensembl, maj 2017
3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
5. Sampson ND, Hewitt JE (Feb 2003). "SF4 and SFRS14, two related putative splicing factors on human chromosome 19p13.11". Gene. 305 (1): 91–100. doi:10.1016/S0378-1119(02)01230-1. PMID 12594045.
6. "Entrez Gene: SFRS14 splicing factor, arginine/serine-rich 14".
7. Nagase, T., Ishikawa, K., Nakajima, D., Ohira, M., Seki, N., Miyajima, N., Tanaka, A., Kotani, H., Nomura, N., Ohara, O. Prediction of the coding sequences of unidentified human genes. VII. The complete sequences of 100 new cDNA clones from brain which can code for large proteins in vitro. DNA Res. 4: 141-150, 1997. [PubMed]: 9205841
8. Sampson, N. D., Hewitt, J. E. SF4 and SFRS14, two related putative splicing factors on human chromosome 19p13.11. Gene 305: 91-100, 2003. [PubMed]: 12594045

Dopunska literatura

• Lim J, Hao T, Shaw C, et al. (2006). "A protein-protein interaction network for human inherited ataxias and disorders of Purkinje cell degeneration". Cell. 125 (4): 801–14. doi:10.1016/j.cell.2006.03.032. PMID 16713569. S2CID 13709685.
• Nousiainen M, Silljé HH, Sauer G, et al. (2006). "Phosphoproteome analysis of the human mitotic spindle". Proc. Natl. Acad. Sci. U.S.A. 103 (14): 5391–6. doi:10.1073/pnas.0507066103. PMC 1459365. PMID 16565220.
• Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The Status, Quality, and Expansion of the NIH Full-Length cDNA Project: The Mammalian Gene Collection (MGC)". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC 528928. PMID 15489334.
• Beausoleil SA, Jedrychowski M, Schwartz D, et al. (2004). "Large-scale characterization of HeLa cell nuclear phosphoproteins". Proc. Natl. Acad. Sci. U.S.A. 101 (33): 12130–5. doi:10.1073/pnas.0404720101. PMC 514446. PMID 15302935.
• Grimwood J, Gordon LA, Olsen A, et al. (2004). "The DNA sequence and biology of human chromosome 19". Nature. 428 (6982): 529–35. doi:10.1038/nature02399. PMID 15057824.
• Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932.
• Nagase T, Ishikawa K, Nakajima D, et al. (1997). "Prediction of the coding sequences of unidentified human genes. VII. The complete sequences of 100 new cDNA clones from brain which can code for large proteins in vitro". DNA Res. 4 (2): 141–50. doi:10.1093/dnares/4.2.141. PMID 9205841.

Vanjski linkovi

• SUGP2 lokacija ljudskog genoma UCSC Genome Browser.

SUGP2 detalji ljudskog genoma u UCSC Genome Browser.