RAD23A

Protein RAD23 homologa A UV ekscizijskog popravka jest protein koji je kod ljudi kodiran genom RAD23A sa p kraka hromosoma 19.^[5]

RAD23A
Dostupne strukture PDB Pretraga ortologa: PDBe RCSB Spisak PDB ID kodova 1DV0, 1F4I, 1IFY, 1OQY, 1P98, 1P9D, 1QZE, 1TP4, 2WYQ
Identifikatori
Aliasi	RAD23A
Vanjski ID-jevi	OMIM: 600061 MGI: 105126 HomoloGene: 48322 GeneCards: RAD23A
Lokacija gena (čovjek) Hrom. Hromosom 19 (čovjek)[1] Bend 19p13.13 Početak 12,945,855 bp[1] Kraj 12,953,642 bp[1]
Lokacija gena (miš) Hrom. Hromosom 8 (miš)[2] Bend 8|8 C3 Početak 85,560,648 bp[2] Kraj 85,567,294 bp[2]
Obrazac RNK ekspresije Više referentnih podataka o ekspresiji
Ontologija gena Molekularna funkcija • single-stranded DNA binding • ubiquitin-specific protease binding • oštećeno vezivanje sa DNK • polyubiquitin modification-dependent protein binding • GO:0001948, GO:0016582 vezivanje za proteine • kinase binding • ubiquitin binding • proteasome binding Ćelijska komponenta • Proteasom • nukleoplazma • centar organizacije mikrotubula • citosol • jedro • citoplazma • intracellular membrane-bounded organelle • GO:0009327 makromolekulani kompleks Biološki proces • Popravak ekscizijom nukleotida • positive regulation of viral genome replication • GO:0022415 viral process • GO:0100026 Popravka DNK • cellular response to DNA damage stimulus • proteasome-mediated ubiquitin-dependent protein catabolic process • regulation of proteasomal ubiquitin-dependent protein catabolic process • protein deubiquitination • protein destabilization • positive regulation of proteasomal ubiquitin-dependent protein catabolic process • positive regulation of cell cycle Izvori:Amigo / QuickGO
Ortolozi
Vrste	Čovjek	Miš
Entrez	5886	19358
Ensembl	ENSG00000179262	ENSMUSG00000003813
UniProt	P54725	P54726
RefSeq (mRNK)	NM_001270362 NM_001270363 NM_005053	NM_001297606 NM_001297607 NM_009010 NM_001378894 NM_001378895 NM_001378896
RefSeq (bjelančevina)	NP_001257291 NP_001257292 NP_005044	NP_001284535 NP_001284536 NP_033036 NP_001365823 NP_001365824 NP_001365825
Lokacija (UCSC)	Chr 19: 12.95 – 12.95 Mb	Chr 8: 85.56 – 85.57 Mb
PubMed pretraga	[3]	[4]
Wikipodaci
Pogledaj/uredi – čovjek Pogledaj/uredi – miš

Aminokiselinska sekvenca

Dužina polipeptidnog lanca je 363 aminokiseline, a molekulska težina 39.609 Da.^[6]

• C: Cistein
• D: Asparaginska kiselina
• E: Glutaminska kiselina
• F: Fenilalanin
• G: Glicin
• H: Histidin
• I: Izoleucin
• K: Lizin
• L: Leucin
• M: Metionin
• N: Asparagin
• P: Prolin
• Q: Glutamin
• R: Arginin
• S: Serin
• T: Treonin
• V: Valin
• W: Triptofan
• Y: Tirozin

10		20		30		40		50
MAVTITLKTL		QQQTFKIRME		PDETVKVLKE		KIEAEKGRDA		FPVAGQKLIY
AGKILSDDVP		IRDYRIDEKN		FVVVMVTKTK		AGQGTSAPPE		ASPTAAPESS
TSFPPAPTSG		MSHPPPAARE		DKSPSEESAP		TTSPESVSGS		VPSSGSSGRE
EDAASTLVTG		SEYETMLTEI		MSMGYERERV		VAALRASYNN		PHRAVEYLLT
GIPGSPEPEH		GSVQESQVSE		QPATEAAGEN		PLEFLRDQPQ		FQNMRQVIQQ
NPALLPALLQ		QLGQENPQLL		QQISRHQEQF		IQMLNEPPGE		LADISDVEGE
VGAIGEEAPQ		MNYIQVTPQE		KEAIERLKAL		GFPESLVIQA		YFACEKNENL
AANFLLSQNF		DDE

Funkcija

Protein kodiran ovim genom je jedan od dva ljudska homologa Saccharomyces cerevisiae Rad23, proteina uključenog u popravak nukleotidnom ekscizijom (NER). Pokazalo se da ovaj protein stupa u interakciju sa i podiže ekscizijsku aktivnost nukleotida 3-metiladenin-DNK glikozilaze (MPG), što ukazuje na ulogu u prepoznavanju oštećenja DNK u ekscizijskoj popravci baza. Ovaj protein sadrži N-terminalni domen sličan ubikvitinu, za koji je objavljeno da interraguje sa proteasomom 265, kao i sa ubikvitinskom protein-ligazom E6AP, i stoga sugeriše da bi ovaj protein mogao biti uključeni u proteolitski put u ćelijama posredovan ubikvitinom^[6]

Interakcije

Pokazano je da RAD23A interraguje sa:

• Ataksin 3,
• Ataxin 3,^[7]
• PSMD4,^[8][9] i
• Sekvestosom 1.^[10]

Reference

1. GRCh38: Ensembl release 89: ENSG00000179262 - Ensembl, maj 2017
2. GRCm38: Ensembl release 89: ENSMUSG00000003813 - Ensembl, maj 2017
3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
5. van der Spek PJ, Smit EM, Beverloo HB, Sugasawa K, Masutani C, Hanaoka F, Hoeijmakers JH, Hagemeijer A (mart 1995). "Chromosomal localization of three repair genes: the xeroderma pigmentosum group C gene and two human homologs of yeast RAD23". Genomics. 23 (3): 651–658. doi:10.1006/geno.1994.1554. hdl:1765/3069. PMID 7851894.
6. "Entrez Gene: RAD23A RAD23 homolog A (S. cerevisiae)"..
7. Wang G, Sawai N, Kotliarova S, Kanazawa I, Nukina N (juli 2000). "Ataxin-3, the MJD1 gene product, interacts with the two human homologs of yeast DNA repair protein RAD23, HHR23A and HHR23B". Hum. Mol. Genet. 9 (12): 1795–803. doi:10.1093/hmg/9.12.1795. PMID 10915768.
8. Hiyama H, Yokoi M, Masutani C, Sugasawa K, Maekawa T, Tanaka K, Hoeijmakers JH, Hanaoka F (septembar 1999). "Interaction of hHR23 with S5a. The ubiquitin-like domain of hHR23 mediates interaction with S5a subunit of 26 S proteasome". J. Biol. Chem. 274 (39): 28019–25. doi:10.1074/jbc.274.39.28019. PMID 10488153.
9. Mueller TD, Feigon J (septembar 2003). "Structural determinants for the binding of ubiquitin-like domains to the proteasome". EMBO J. 22 (18): 4634–45. doi:10.1093/emboj/cdg467. PMC 212733. PMID 12970176.
10. Rual JF, Venkatesan K, Hao T, Hirozane-Kishikawa T, Dricot A, Li N, Berriz GF, Gibbons FD, Dreze M, Ayivi-Guedehoussou N, Klitgord N, Simon C, Boxem M, Milstein S, Rosenberg J, Goldberg DS, Zhang LV, Wong SL, Franklin G, Li S, Albala JS, Lim J, Fraughton C, Llamosas E, Cevik S, Bex C, Lamesch P, Sikorski RS, Vandenhaute J, Zoghbi HY, Smolyar A, Bosak S, Sequerra R, Doucette-Stamm L, Cusick ME, Hill DE, Roth FP, Vidal M (oktobar 2005). "Towards a proteome-scale map of the human protein-protein interaction network". Nature. 437 (7062): 1173–8. Bibcode:2005Natur.437.1173R. doi:10.1038/nature04209. PMID 16189514. S2CID 4427026.

Dopunska literatura

• Eichbaum QG, Beatty DW, Parker MI (1994). "Identification of cardiac autoantigens in human heart cDNA libraries using acute rheumatic fever sera". J. Autoimmun. 7 (2): 243–261. doi:10.1006/jaut.1994.1019. PMID 8037842.
• Masutani C, Sugasawa K, Yanagisawa J, Sonoyama T, Ui M, Enomoto T, Takio K, Tanaka K, van der Spek PJ, Bootsma D (1994). "Purification and cloning of a nucleotide excision repair complex involving the xeroderma pigmentosum group C protein and a human homologue of yeast RAD23". EMBO J. 13 (8): 1831–43. doi:10.1002/j.1460-2075.1994.tb06452.x. PMC 395023. PMID 8168482.
• van der Spek PJ, Eker A, Rademakers S, Visser C, Sugasawa K, Masutani C, Hanaoka F, Bootsma D, Hoeijmakers JH (1996). "XPC and human homologs of RAD23: intracellular localization and relationship to other nucleotide excision repair complexes". Nucleic Acids Res. 24 (13): 2551–2559. doi:10.1093/nar/24.13.2551. PMC 145966. PMID 8692695.
• van der Spek PJ, Visser CE, Hanaoka F, Smit B, Hagemeijer A, Bootsma D, Hoeijmakers JH (1996). "Cloning, comparative mapping, and RNA expression of the mouse homologues of the Saccharomyces cerevisiae nucleotide excision repair gene RAD23". Genomics. 31 (1): 20–27. doi:10.1006/geno.1996.0004. hdl:1765/3097. PMID 8808275.
• Li L, Lu X, Peterson C, Legerski R (1997). "XPC interacts with both HHR23B and HHR23A in vivo". Mutat. Res. 383 (3): 197–203. doi:10.1016/s0921-8777(97)00002-5. PMID 9164480.
• Withers-Ward ES, Jowett JB, Stewart SA, Xie YM, Garfinkel A, Shibagaki Y, Chow SA, Shah N, Hanaoka F, Sawitz DG, Armstrong RW, Souza LM, Chen IS (1997). "Human immunodeficiency virus type 1 Vpr interacts with HHR23A, a cellular protein implicated in nucleotide excision DNA repair". J. Virol. 71 (12): 9732–42. doi:10.1128/JVI.71.12.9732-9742.1997. PMC 230283. PMID 9371639.
• Schauber C, Chen L, Tongaonkar P, Vega I, Lambertson D, Potts W, Madura K (1998). "Rad23 links DNA repair to the ubiquitin/proteasome pathway". Nature. 391 (6668): 715–718. Bibcode:1998Natur.391..715S. doi:10.1038/35661. PMID 9490418. S2CID 4417020.
• Gragerov A, Kino T, Ilyina-Gragerova G, Chrousos GP, Pavlakis GN (1998). "HHR23A, the human homologue of the yeast repair protein RAD23, interacts specifically with Vpr protein and prevents cell cycle arrest but not the transcriptional effects of Vpr". Virology. 245 (2): 323–330. doi:10.1006/viro.1998.9138. PMID 9636371.
• Dieckmann T, Withers-Ward ES, Jarosinski MA, Liu CF, Chen IS, Feigon J (1998). "Structure of a human DNA repair protein UBA domain that interacts with HIV-1 Vpr". Nat. Struct. Biol. 5 (12): 1042–1047. doi:10.1038/4220. PMID 9846873. S2CID 30478711.
• Kumar S, Talis AL, Howley PM (1999). "Identification of HHR23A as a substrate for E6-associated protein-mediated ubiquitination". J. Biol. Chem. 274 (26): 18785–18792. doi:10.1074/jbc.274.26.18785. PMID 10373495.
• Hiyama H, Yokoi M, Masutani C, Sugasawa K, Maekawa T, Tanaka K, Hoeijmakers JH, Hanaoka F (1999). "Interaction of hHR23 with S5a. The ubiquitin-like domain of hHR23 mediates interaction with S5a subunit of 26 S proteasome". J. Biol. Chem. 274 (39): 28019–28025. doi:10.1074/jbc.274.39.28019. PMID 10488153.
• Miao F, Bouziane M, Dammann R, Masutani C, Hanaoka F, Pfeifer G, O'Connor TR (2000). "3-Methyladenine-DNA glycosylase (MPG protein) interacts with human RAD23 proteins". J. Biol. Chem. 275 (37): 28433–28438. doi:10.1074/jbc.M001064200. PMID 10854423.
• Wang G, Sawai N, Kotliarova S, Kanazawa I, Nukina N (2000). "Ataxin-3, the MJD1 gene product, interacts with the two human homologs of yeast DNA repair protein RAD23, HHR23A and HHR23B". Hum. Mol. Genet. 9 (12): 1795–1803. doi:10.1093/hmg/9.12.1795. PMID 10915768.
• Withers-Ward ES, Mueller TD, Chen IS, Feigon J (2001). "Biochemical and structural analysis of the interaction between the UBA(2) domain of the DNA repair protein HHR23A and HIV-1 Vpr". Biochemistry. 39 (46): 14103–14112. doi:10.1021/bi0017071. PMID 11087358.
• Zhu Q, Wani G, Wani MA, Wani AA (2001). "Human homologue of yeast Rad23 protein A interacts with p300/cyclic AMP-responsive element binding (CREB)-binding protein to down-regulate transcriptional activity of p53". Cancer Res. 61 (1): 64–70. PMID 11196199.
• Mansky LM, Preveral S, Le Rouzic E, Bernard LC, Selig L, Depienne C, Benarous R, Benichou S (2001). "Interaction of human immunodeficiency virus type 1 Vpr with the HHR23A DNA repair protein does not correlate with multiple biological functions of Vpr". Virology. 282 (1): 176–185. doi:10.1006/viro.2000.0791. PMID 11259200.
• Gaynor EM, Chen IS (2001). "Analysis of apoptosis induced by HIV-1 Vpr and examination of the possible role of the hHR23A protein". Exp. Cell Res. 267 (2): 243–257. doi:10.1006/excr.2001.5247. PMID 11426943.
• Chen L, Shinde U, Ortolan TG, Madura K (2002). "Ubiquitin-associated (UBA) domains in Rad23 bind ubiquitin and promote inhibition of multi-ubiquitin chain assembly". EMBO Rep. 2 (10): 933–938. doi:10.1093/embo-reports/kve203. PMC 1084081. PMID 11571271.
• Elder RT, Song XQ, Chen M, Hopkins KM, Lieberman HB, Zhao Y (2002). "Involvement of rhp23, a Schizosaccharomyces pombe homolog of the human HHR23A and Saccharomyces cerevisiae RAD23 nucleotide excision repair genes, in cell cycle control and protein ubiquitination". Nucleic Acids Res. 30 (2): 581–591. doi:10.1093/nar/30.2.581. PMC 99819. PMID 11788722.

Vanjski linkovi