Podjedinica 4 CCR4-NOT transkripcijskog kompleksa je protein koji je kod ljudi kodiran genom CNOT4.[5]
Kratke činjenice Dostupne strukture, PDB ...
Zatvori
Dužina polipeptidnog lanca je 575 aminokiselina, а molekulska težina 63.510 Da.[6]
| 10 | | 20 | | 30 | | 40 | | 50 |
|---|
| MSRSPDAKED | | PVECPLCMEP | | LEIDDINFFP | | CTCGYQICRF | | CWHRIRTDEN |
| GLCPACRKPY | | PEDPAVYKPL | | SQEELQRIKN | | EKKQKQNERK | | QKISENRKHL |
| ASVRVVQKNL | | VFVVGLSQRL | | ADPEVLKRPE | | YFGKFGKIHK | | VVINNSTSYA |
| GSQGPSASAY | | VTYIRSEDAL | | RAIQCVNNVV | | VDGRTLKASL | | GTTKYCSYFL |
| KNMQCPKPDC | | MYLHELGDEA | | ASFTKEEMQA | | GKHQEYEQKL | | LQELYKLNPN |
| FLQLSTGSVD | | KNKNKVTPLQ | | RYDTPIDKPS | | DSLSIGNGDN | | SQQISNSDTP |
| SPPPGLSKSN | | PVIPISSSNH | | SARSPFEGAV | | TESQSLFSDN | | FRHPNPIPSG |
| LPPFPSSPQT | | SSDWPTAPEP | | QSLFTSETIP | | VSSSTDWQAA | | FGFGSSKQPE |
| DDLGFDPFDV | | TRKALADLIE | | KELSVQDQPS | | LSPTSLQNSS | | SHTTTAKGPG |
| SGFLHPAAAT | | NANSLNSTFS | | VLPQRFPQFQ | | QHRAVYNSFS | | FPGQAARYPW |
| MAFPRNSIMH | | LNHTANPTSN | | SNFLDLNLPP | | QHNTGLGGIP | | VAGEEEVKVS |
| TMPLSTSSHS | | LQQGQQPTSL | | HTTVA |
Protein kodiran ovim genom podjedinica je kompleksa CCR4-NOT, globalnog transkripcijskog regulatora i deadenilazatora. Kodirani protein stupa u interakciju s CNOT1 i ima aktivnost ubikvitin-ligaza. Za ovaj gen pronađeno je nekoliko varijanti transkripta, koje kodiraju različite izoforme. (prema bRefSeq, juli 2010). Bolesti povezane sa CNOT4 uključuju adenoiditis.[7]
- Grönholm J, Kaustio M, Myllymäki H, Kallio J, Saarikettu J, Kronhamn J, Valanne S, Silvennoinen O, Rämet M (mart 2012). "Not4 enhances JAK/STAT pathway-dependent gene expression in Drosophila and in human cells". FASEB Journal. 26 (3): 1239–50. doi:10.1096/fj.11-195875. PMID 22159038. S2CID 25618632.
- Winkler GS, Albert TK, Dominguez C, Legtenberg YI, Boelens R, Timmers HT (mart 2004). "An altered-specificity ubiquitin-conjugating enzyme/ubiquitin-protein ligase pair". Journal of Molecular Biology. 337 (1): 157–65. doi:10.1016/j.jmb.2004.01.031. PMID 15001359.
- Lau NC, Kolkman A, van Schaik FM, Mulder KW, Pijnappel WW, Heck AJ, Timmers HT (septembar 2009). "Human Ccr4-Not complexes contain variable deadenylase subunits" (PDF). The Biochemical Journal. 422 (3): 443–53. doi:10.1042/BJ20090500. PMID 19558367.
- Albert TK, Hanzawa H, Legtenberg YI, de Ruwe MJ, van den Heuvel FA, Collart MA, Boelens R, Timmers HT (februar 2002). "Identification of a ubiquitin-protein ligase subunit within the CCR4-NOT transcription repressor complex". The EMBO Journal. 21 (3): 355–64. doi:10.1093/emboj/21.3.355. PMC 125831. PMID 11823428.
- Dominguez C, Bonvin AM, Winkler GS, van Schaik FM, Timmers HT, Boelens R (april 2004). "Structural model of the UbcH5B/CNOT4 complex revealed by combining NMR, mutagenesis, and docking approaches". Structure. 12 (4): 633–44. doi:10.1016/j.str.2004.03.004. PMID 15062086.
- Wistow G, Bernstein SL, Wyatt MK, Fariss RN, Behal A, Touchman JW, Bouffard G, Smith D, Peterson K (juni 2002). "Expressed sequence tag analysis of human RPE/choroid for the NEIBank Project: over 6000 non-redundant transcripts, novel genes and splice variants". Molecular Vision. 8: 205–20. PMID 12107410.
- Mersman DP, Du HN, Fingerman IM, South PF, Briggs SD (april 2009). "Polyubiquitination of the demethylase Jhd2 controls histone methylation and gene expression". Genes & Development. 23 (8): 951–62. doi:10.1101/gad.1769209. PMC 2675863. PMID 19346402.
- Hanzawa H, de Ruwe MJ, Albert TK, van Der Vliet PC, Timmers HT, Boelens R (mart 2001). "The structure of the C4C4 ring finger of human NOT4 reveals features distinct from those of C3HC4 RING fingers". The Journal of Biological Chemistry. 276 (13): 10185–90. doi:10.1074/jbc.M009298200. PMID 11087754.
- Albert TK, Lemaire M, van Berkum NL, Gentz R, Collart MA, Timmers HT (februar 2000). "Isolation and characterization of human orthologs of yeast CCR4-NOT complex subunits". Nucleic Acids Research. 28 (3): 809–17. doi:10.1093/nar/28.3.809. PMC 102560. PMID 10637334.
