Protein hromosomske regije kandidatskog gena 8 poznat i kao faktor kalcij-odgovora (CaRF) jest protein koji je kod ljudi kodiran genom ALS2CR8 sa hromosoma 2.[5][6]
Kratke činjenice Identifikatori, Aliasi ...
| ALS2CR8 |
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| Identifikatori |
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| Aliasi | CARF |
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| Vanjski ID-jevi | OMIM: 607586 MGI: 2182269 HomoloGene: 11689 GeneCards: CARF |
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| Ontologija gena |
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| Molekularna funkcija | • GO:0001131, GO:0001151, GO:0001130, GO:0001204 DNA-binding transcription factor activity
• GO:0000980 RNA polymerase II cis-regulatory region sequence-specific DNA binding
• vezivanje sa DNK
• GO:0001077, GO:0001212, GO:0001213, GO:0001211, GO:0001205 DNA-binding transcription activator activity, RNA polymerase II-specific
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| Ćelijska komponenta | • Jedarce
• nukleoplazma
• jedro
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| Biološki proces | • cellular response to calcium ion
• positive regulation of transcription from RNA polymerase II promoter in response to calcium ion
• GO:0009373 regulation of transcription, DNA-templated
• transcription, DNA-templated
• cellular response to potassium ion
• GO:0003257, GO:0010735, GO:1901228, GO:1900622, GO:1904488 positive regulation of transcription by RNA polymerase II
• transcription by RNA polymerase II
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| Izvori:Amigo / QuickGO |
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| Ortolozi |
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| Vrste | Čovjek | Miš |
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| Entrez | | |
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| Ensembl | | |
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| UniProt | | |
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| RefSeq (mRNK) | | |
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| RefSeq (bjelančevina) | | |
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| Lokacija (UCSC) | Chr 2: 202.91 – 202.99 Mb | Chr 1: 60.1 – 60.15 Mb |
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| PubMed pretraga | [3] | [4] |
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| Wikipodaci |
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Zatvori
Dužina polipeptidnog lanca je 725 aminokiselina, a molekulska težina 80.698 Da.[7].
| 10 | | 20 | | 30 | | 40 | | 50 |
|---|
| MEQSNDSLRV | | NHNDGEESKT | | SAQVFEHLIC | | MDSRDSSFGQ | | NDSPTVLPIT |
| TREANNSLIS | | QNIPGPLTQT | | QTLSAEQFHL | | VDQNGQAIQY | | ELQSLGESNA |
| QMMIVASPTE | | NGQVLRVIPP | | TQTGMAQVII | | PQGQLVDVNS | | PRDVPEEKPS |
| NRNLPTVRVD | | TLADNTSNYI | | LHPQTSFPLP | | KKSVTGMLEE | | PLLGPLQPLS |
| SNTPIWACRL | | RSCEKIGDSY | | RGYCVSETEL | | ESVLTFHKQQ | | TQSVWGTRQS |
| PSPAKPATRL | | MWKSQYVPYD | | GIPFVNAGSR | | AVVMECQYGP | | RRKGFQLKKV |
| SEQESRSCQL | | YKATCPARIY | | IKKVQKFPEY | | RVPTDPKIDK | | KIIRMEQEKA |
| FNMLKKNLVD | | AGGVLRWYVQ | | LPTQQAHQYH | | ELETPCLTLS | | PSPFPVSSLE |
| EEETAVRDEN | | CALPSRLHPQ | | VAHKIQELVS | | QGIEQVYAVR | | KQLRKFVERE |
| LFKPDEVPER | | HNLSFFPTVN | | DIKNHIHEVQ | | KSLRNGDTVY | | NSEIIPATLQ |
| WTTDSGNILK | | ETMTVTFAEG | | NSPGESITTK | | VETNQTRGSL | | SPEPTHLLSS |
| LSSFQPKIFT | | QLQGLQLQPR | | YTSPDESPAV | | VSVNNQPSSS | | PSGLLDTIGS |
| AVMNNNSLLL | | GQSHSLQRDT | | CLTQNNSTAS | | TMGNLPEPDQ | | NLVAMDELVE |
| VGDVEDTGNL | | EGTVHRILLG | | DVQTIPIQII | | DNHSALIEEN | | PESTISVSQV |
| KQEPKEPALS | | MEAKKTVDYK | | KLSAT |
Nokautirani gen Carf (Als2cr8) kod miševa dovodi do deficita povezanih s učenjem. Štaviše, pokazalo se da Carf funkcioniše kao transkripcijski faktor koji regulira ekspresiju BDNF.[8]
Hadano S, Hand CK, Osuga H, Yanagisawa Y, Otomo A, Devon RS, Miyamoto N, Showguchi-Miyata J, Okada Y, Singaraja R, Figlewicz DA, Kwiatkowski T, Hosler BA, Sagie T, Skaug J, Nasir J, Brown RH Jr, Scherer SW, Rouleau GA, Hayden MR, Ikeda JE (Oct 2001). "A gene encoding a putative GTPase regulator is mutated in familial amyotrophic lateral sclerosis 2". Nat Genet. 29 (2): 166–73. doi:10.1038/ng1001-166. PMID 11586298. S2CID 52828189.
- Maruyama K, Sugano S (1994). "Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides". Gene. 138 (1–2): 171–4. doi:10.1016/0378-1119(94)90802-8. PMID 8125298.
- Suzuki Y, Yoshitomo-Nakagawa K, Maruyama K, et al. (1997). "Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149.
- Tao X, West AE, Chen WG, et al. (2002). "A calcium-responsive transcription factor, CaRF, that regulates neuronal activity-dependent expression of BDNF". Neuron. 33 (3): 383–95. doi:10.1016/S0896-6273(01)00561-X. PMID 11832226. S2CID 10874584.
- Hasan MK, Yaguchi T, Sugihara T, et al. (2002). "CARF is a novel protein that cooperates with mouse p19ARF (human p14ARF) in activating p53". J. Biol. Chem. 277 (40): 37765–70. doi:10.1074/jbc.M204177200. PMID 12154087.
- Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932.
- Ota T, Suzuki Y, Nishikawa T, et al. (2004). "Complete sequencing and characterization of 21,243 full-length human cDNAs". Nat. Genet. 36 (1): 40–5. doi:10.1038/ng1285. PMID 14702039.
- Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The Status, Quality, and Expansion of the NIH Full-Length cDNA Project: The Mammalian Gene Collection (MGC)". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC 528928. PMID 15489334.
- Hillier LW, Graves TA, Fulton RS, et al. (2005). "Generation and annotation of the DNA sequences of human chromosomes 2 and 4". Nature. 434 (7034): 724–31. doi:10.1038/nature03466. PMID 15815621.
- Rual JF, Venkatesan K, Hao T, et al. (2005). "Towards a proteome-scale map of the human protein-protein interaction network". Nature. 437 (7062): 1173–8. doi:10.1038/nature04209. PMID 16189514. S2CID 4427026.
- Kimura K, Wakamatsu A, Suzuki Y, et al. (2006). "Diversification of transcriptional modulation: Large-scale identification and characterization of putative alternative promoters of human genes". Genome Res. 16 (1): 55–65. doi:10.1101/gr.4039406. PMC 1356129. PMID 16344560.
