SRGN

Serglicin, znan i kao hematopoietski proteoglikanski jezgreni protein ili proteoglikanski jezgreni protein sekrecijskih granula, jest protein koji je kod ljudi kodiran genom SRGN.^[5] It is primarily expressed in hematopoietic cells and endothelial cells,^[6] and is the only known intracellular proteoglycan.^[7]

SRGN
Identifikatori
Aliasi	SRGN
Vanjski ID-jevi	OMIM: 177040 MGI: 97756 HomoloGene: 2043 GeneCards: SRGN
Lokacija gena (čovjek) Hrom. Hromosom 10 (čovjek)[1] Bend 10q22.1 Početak 69,088,103 bp[1] Kraj 69,104,805 bp[1]
Lokacija gena (miš) Hrom. Hromosom 10 (miš)[2] Bend 10|10 B4 Početak 62,329,612 bp[2] Kraj 62,363,230 bp[2]
Obrazac RNK ekspresije Više referentnih podataka o ekspresiji
Ontologija gena Molekularna funkcija • GO:0001948, GO:0016582 vezivanje za proteine Ćelijska komponenta • Golđijev aparat • extracellular region • platelet alpha granule lumen • Vanćelijsko • mast cell granule • Schaffer collateral - CA1 synapse • glutamatergic synapse • postsynaptic specialization, intracellular component Biološki proces • negative regulation of bone mineralization • biomineral tissue development • platelet degranulation • protease localization to mast cell secretory granule • granzyme-mediated apoptotic signaling pathway • GO:0097285 apoptoza • protein processing • mast cell secretory granule organization • T cell secretory granule organization • maintenance of protease location in mast cell secretory granule • maintenance of granzyme B location in T cell secretory granule • modulation of chemical synaptic transmission • regulation of postsynapse organization Izvori:Amigo / QuickGO
Ortolozi
Vrste	Čovjek	Miš
Entrez	5552	19073
Ensembl	ENSG00000122862	ENSMUSG00000020077
UniProt	P10124	P13609
RefSeq (mRNK)	NM_002727 NM_001321053 NM_001321054	NM_011157 NM_001358965
RefSeq (bjelančevina)	NP_001307982 NP_001307983 NP_002718	NP_035287 NP_001345894
Lokacija (UCSC)	Chr 10: 69.09 – 69.1 Mb	Chr 10: 62.33 – 62.36 Mb
PubMed pretraga	[3]	[4]
Wikipodaci
Pogledaj/uredi – čovjek Pogledaj/uredi – miš

Aminokiselinska sekvenca

Dužina polipeptidnog lanca je 158 aminokiselina, а molekulska težina 17.652 Da.^[8]

10		20		30		40		50
MMQKLLKCSR		LVLALALILV		LESSVQGYPT		RRARYQWVRC		NPDSNSANCL
EEKGPMFELL		PGESNKIPRL		RTDLFPKTRI		QDLNRIFPLS		EDYSGSGFGS
GSGSGSGSGS		GFLTEMEQDY		QLVDESDAFH		DNLRSLDRNL		PSDSQDLGQH
GLEEDFML

C: Cistein

D: Asparaginska kiselina

E: Glutaminska kiselina

F: Fenilalanin

G: Glicin

H: Histidin

I: Izoleucin

K: Lizin

L: Leucin

M: Metionin

N: Asparagin

P: Prolin

Q: Glutamin

R: Arginin

S: Serin

T: Treonin

V: Valin

W: Triptofan

Y: Tirozin

Funkcija

Ovaj gen kodira protein najpoznatiji kao hematopoetska ćelija proteoglikanskih granula. Proteoglikani pohranjeni u sekrecijskim granulama mnogih hematopoetskih ćelija također sadrže peptidno jezgro otporno na proteazu, koja može biti važna za neutraliziranje hidrolitiskih enzima. Utvrđeno je da je ovaj kodirani protein povezan s makromolekulnim kompleksom granzima i perforina, koji može poslužiti kao posrednik apoptoza posredovanih granulama.^[5]

Reference

1. GRCh38: Ensembl release 89: ENSG00000122862 - Ensembl, maj 2017
2. GRCm38: Ensembl release 89: ENSMUSG00000020077 - Ensembl, maj 2017
3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
5. "Entrez Gene: SRGN serglycin".
6. Kolset SO, Tveit H (april 2008). "Serglycin--structure and biology". Cellular and Molecular Life Sciences. 65 (7–8): 1073–85. doi:10.1007/s00018-007-7455-6. PMID 18066495. S2CID 19422023.
7. Iozzo RV, Schaefer L (mart 2015). "Proteoglycan form and function: A comprehensive nomenclature of proteoglycans". Matrix Biology. 42: 11–55. doi:10.1016/j.matbio.2015.02.003. PMC 4859157. PMID 25701227.
8. "UniProt, P10124". Pristupljeno 5. 9. 2021.

Dopunska literatura

• Schick BP (2000). "Regulation of expression of megakaryocyte and platelet proteoglycans". Stem Cells. 14 Suppl 1: 220–31. doi:10.1002/stem.5530140729. PMID 11012225. S2CID 24328770.
• Humphries DE, Nicodemus CF, Schiller V, Stevens RL (juli 1992). "The human serglycin gene. Nucleotide sequence and methylation pattern in human promyelocytic leukemia HL-60 cells and T-lymphoblast Molt-4 cells". The Journal of Biological Chemistry. 267 (19): 13558–63. PMID 1377686.
• Nicodemus CF, Avraham S, Austen KF, Purdy S, Jablonski J, Stevens RL (april 1990). "Characterization of the human gene that encodes the peptide core of secretory granule proteoglycans in promyelocytic leukemia HL-60 cells and analysis of the translated product". The Journal of Biological Chemistry. 265 (10): 5889–96. PMID 2180935.
• Mattei MG, Périn JP, Alliel PM, Bonnet F, Maillet P, Passage E, Mattei JF, Jollès P (april 1989). "Localization of human platelet proteoglycan gene to chromosome 10, band q22.1, by in situ hybridization". Human Genetics. 82 (1): 87–8. doi:10.1007/BF00288281. PMID 2714783. S2CID 1378271.
• Avraham S, Stevens RL, Nicodemus CF, Gartner MC, Austen KF, Weis JH (maj 1989). "Molecular cloning of a cDNA that encodes the peptide core of a mouse mast cell secretory granule proteoglycan and comparison with the analogous rat and human cDNA". Proceedings of the National Academy of Sciences of the United States of America. 86 (10): 3763–7. doi:10.1073/pnas.86.10.3763. PMC 287220. PMID 2726751.
• Stellrecht CM, Saunders GF (septembar 1989). "Nucleotide sequence of a cDNA encoding a hemopoietic proteoglycan core protein". Nucleic Acids Research. 17 (18): 7523. doi:10.1093/nar/17.18.7523. PMC 334837. PMID 2798108.
• Stevens RL, Avraham S, Gartner MC, Bruns GA, Austen KF, Weis JH (maj 1988). "Isolation and characterization of a cDNA that encodes the peptide core of the secretory granule proteoglycan of human promyelocytic leukemia HL-60 cells". The Journal of Biological Chemistry. 263 (15): 7287–91. PMID 2835370.
• Périn JP, Bonnet F, Maillet P, Jollès P (novembar 1988). "Characterization and N-terminal sequence of human platelet proteoglycan". The Biochemical Journal. 255 (3): 1007–13. doi:10.1042/bj2551007. PMC 1135341. PMID 3214420.
• Avraham S, Stevens RL, Gartner MC, Austen KF, Lalley PA, Weis JH (maj 1988). "Isolation of a cDNA that encodes the peptide core of the secretory granule proteoglycan of rat basophilic leukemia-1 cells and assessment of its homology to the human analogue". The Journal of Biological Chemistry. 263 (15): 7292–6. PMID 3366780.
• Alliel PM, Périn JP, Maillet P, Bonnet F, Rosa JP, Jollès P (august 1988). "Complete amino acid sequence of a human platelet proteoglycan". FEBS Letters. 236 (1): 123–6. doi:10.1016/0014-5793(88)80298-9. PMID 3402609. S2CID 8581282.
• Hatton MN, Loomis RE, Levine MJ, Tabak LA (septembar 1985). "Masticatory lubrication. The role of carbohydrate in the lubricating property of a salivary glycoprotein-albumin complex". The Biochemical Journal. 230 (3): 817–20. doi:10.1042/bj2300817. PMC 1152688. PMID 4062880.
• Schick BP, Jacoby JA (oktobar 1995). "Serglycin and betaglycan proteoglycans are expressed in the megakaryocytic cell line CHRF 288-11 and normal human megakaryocytes". Journal of Cellular Physiology. 165 (1): 96–106. doi:10.1002/jcp.1041650113. PMID 7559813. S2CID 1455744.
• Kato S, Sekine S, Oh SW, Kim NS, Umezawa Y, Abe N, Yokoyama-Kobayashi M, Aoki T (decembar 1994). "Construction of a human full-length cDNA bank". Gene. 150 (2): 243–50. doi:10.1016/0378-1119(94)90433-2. PMID 7821789.
• Kolset SO, Mann DM, Uhlin-Hansen L, Winberg JO, Ruoslahti E (april 1996). "Serglycin-binding proteins in activated macrophages and platelets". Journal of Leukocyte Biology. 59 (4): 545–54. doi:10.1002/jlb.59.4.545. PMID 8613703. S2CID 23125881.
• Toyama-Sorimachi N, Kitamura F, Habuchi H, Tobita Y, Kimata K, Miyasaka M (oktobar 1997). "Widespread expression of chondroitin sulfate-type serglycins with CD44 binding ability in hematopoietic cells". The Journal of Biological Chemistry. 272 (42): 26714–9. doi:10.1074/jbc.272.42.26714. PMID 9334256.
• Galvin JP, Spaeny-Dekking LH, Wang B, Seth P, Hack CE, Froelich CJ (maj 1999). "Apoptosis induced by granzyme B-glycosaminoglycan complexes: implications for granule-mediated apoptosis in vivo". Journal of Immunology. 162 (9): 5345–50. PMID 10228010.
• Schick BP, Gradowski JF, San Antonio JD (januar 2001). "Synthesis, secretion, and subcellular localization of serglycin proteoglycan in human endothelial cells". Blood. 97 (2): 449–58. doi:10.1182/blood.V97.2.449. PMID 11154222.
• Omtvedt LA, Kolset SO, Thoen J, Førre Y, Gill MR (2001). "Serglycin expression in CD2+ and CD14+ cells from patients with various rheumatic diseases". Scandinavian Journal of Rheumatology. 30 (3): 164–6. doi:10.1080/030097401300162941. PMID 11469527. S2CID 218854509.
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