Ras supresorski protein 1 je protein koji je kod ljudi kodiran genom RSU1.[5][6]
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Zatvori
Ovaj gen kodira protein koji je uključen u put transdukcije Ras signala, inhibiciju rasta i procese diferencijacije izazvane nervnim faktorom rasta, kako je utvrđeno u studijama mišje ćelijske linije. Kod miša, kodirani protein je u početku izoliran na osnovu njegove sposobnosti da inhibira v-Ras transformaciju. Prijavljeno je više alternativno prerađenih varijanti transkripta za ovaj gen; jedna od ovih varijanti pronađena je samo u tumorima glioma.[6]
Također se vidi da RSU-1 djeluje kao strukturni protein u integrinski posredovanim kompleksima fokusne adhezije. Snažno se veže za protein PINCH.
Dužina polipeptidnog lanca je 277 aminokiselina, а molekulska težina 31.540 Da.[7]
| 10 | | 20 | | 30 | | 40 | | 50 |
|---|
| MSKSLKKLVE | | ESREKNQPEV | | DMSDRGISNM | | LDVNGLFTLS | | HITQLVLSHN |
| KLTMVPPNIA | | ELKNLEVLNF | | FNNQIEELPT | | QISSLQKLKH | | LNLGMNRLNT |
| LPRGFGSLPA | | LEVLDLTYNN | | LSENSLPGNF | | FYLTTLRALY | | LSDNDFEILP |
| PDIGKLTKLQ | | ILSLRDNDLI | | SLPKEIGELT | | QLKELHIQGN | | RLTVLPPELG |
| NLDLTGQKQV | | FKAENNPWVT | | PIADQFQLGV | | SHVFEYIRSE | | TYKYLYGRHM |
| QANPEPPKKN | | NDKSKKISRK | | PLAAKNR |
- Cutler ML, Bassin RH, Zanoni L, Talbot N (1992). "Isolation of rsp-1, a novel cDNA capable of suppressing v-Ras transformation". Mol. Cell. Biol. 12 (9): 3750–6. doi:10.1128/MCB.12.9.3750. PMC 360236. PMID 1508180.
- Tsuda T, Marinetti MR, Masuelli L, Cutler ML (1995). "The Ras suppressor RSU-1 localizes to 10p13 and its expression in the U251 glioblastoma cell line correlates with a decrease in growth rate and tumorigenic potential". Oncogene. 11 (2): 397–403. PMID 7624154.
- Matoba R, Okubo K, Hori N, et al. (1994). "The addition of 5'-coding information to a 3'-directed cDNA library improves analysis of gene expression". Gene. 146 (2): 199–207. doi:10.1016/0378-1119(94)90293-3. PMID 8076819.
- Maruyama K, Sugano S (1994). "Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides". Gene. 138 (1–2): 171–4. doi:10.1016/0378-1119(94)90802-8. PMID 8125298.
- Masuelli L, Cutler ML (1996). "Increased expression of the Ras suppressor Rsu-1 enhances Erk-2 activation and inhibits Jun kinase activation". Mol. Cell. Biol. 16 (10): 5466–76. doi:10.1128/MCB.16.10.5466. PMC 231547. PMID 8816460.
- Suzuki Y, Yoshitomo-Nakagawa K, Maruyama K, et al. (1997). "Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149.
- Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932.
- Chunduru S, Kawami H, Gullick R, et al. (2003). "Identification of an alternatively spliced RNA for the Ras suppressor RSU-1 in human gliomas". J. Neurooncol. 60 (3): 201–11. doi:10.1023/A:1021130620178. PMID 12510772. S2CID 34539.
- Gevaert K, Goethals M, Martens L, et al. (2004). "Exploring proteomes and analyzing protein processing by mass spectrometric identification of sorted N-terminal peptides". Nat. Biotechnol. 21 (5): 566–9. doi:10.1038/nbt810. PMID 12665801. S2CID 23783563.
- Ota T, Suzuki Y, Nishikawa T, et al. (2004). "Complete sequencing and characterization of 21,243 full-length human cDNAs". Nat. Genet. 36 (1): 40–5. doi:10.1038/ng1285. PMID 14702039.
- Aboulaich N, Vainonen JP, Strålfors P, Vener AV (2005). "Vectorial proteomics reveal targeting, phosphorylation and specific fragmentation of polymerase I and transcript release factor (PTRF) at the surface of caveolae in human adipocytes". Biochem. J. 383 (Pt 2): 237–48. doi:10.1042/BJ20040647. PMC 1134064. PMID 15242332.
- Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The Status, Quality, and Expansion of the NIH Full-Length cDNA Project: The Mammalian Gene Collection (MGC)". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC 528928. PMID 15489334.
- Dougherty GW, Chopp T, Qi SM, Cutler ML (2005). "The Ras suppressor Rsu-1 binds to the LIM 5 domain of the adaptor protein PINCH1 and participates in adhesion-related functions". Exp. Cell Res. 306 (1): 168–79. doi:10.1016/j.yexcr.2005.01.025. PMID 15878342.
- Ewing RM, Chu P, Elisma F, et al. (2007). "Large-scale mapping of human protein–protein interactions by mass spectrometry". Mol. Syst. Biol. 3 (1): 89. doi:10.1038/msb4100134. PMC 1847948. PMID 17353931.
