Prtein 5 programirane ćelijske smrti je protein koji jr kod ljudi kodiran genom PDCD5.[4][5]
Kratke činjenice Dostupne strukture, PDB ...
Zatvori
Ovaj gen kodira protein sa ekspresijom u tumorskim ćelijama tokom apoptoze, neovisno od stimulusa koji je induciraju. Prije indukcije apoptoze, ovaj genski proizvod distribuira se i u jedru i citoplazmi.
Kada se inducira apoptoza, nivo ovog proteina raste i prelaskom iz citoplazme akumulira se u jedru. Iako njegova tačna funkcija nije definirana, smatra se da ovaj protein ima ranu i univerzalnu ulogu u apoptozi.[5]
Koristeći analizu razlika predstavnika cDNK, Liu et al. (1999) identificirali su novi gen, označen kao TFAR19, iz ćelija TF1 (ljudske premeloidne ćelijske linije, uspostavljene od pacijenta sa eritroleukemijom) koje su podvrgnute apoptozi, nakon povlačenja GM-CSF iz medija za kulturu ćelija. Gen TFAR19 kodira protein od 125 aminokiselina, sa ATG inicijacijskim kodonom na nukleotidima 25-27. Sadrži mjesto fosforilacije protein-kinaze ovisne o cAMP-u i cGMP-u, više mjesta fosforilacije protein-kinaze C i mjesto fosforilacije kazein-kinaze II. Dijeli značajnu homologiju s odgovarajućim proteinima nekih vrsta, od kvasaca do miševa. Predviđa se je protein TFAR19 lokaliziran u jedru. Analiza točkastih obojenja pokazala je da se TFAR19 sveprisutno eksprimira, ali je najistaknutiji u srcu, sjemenici, bubrezima, hipofizi, [[nadbubrežna žlijezda|nadbubrežnoj žlijezdi i posteljici. Ekspresija u fetusnim tkivima je znatno niža od ekspresije u tkivima odraslih. Ekspresija TFAR19 je pojačano regulirana u ćelijama tumora koje su u apoptozi i pojačava apoptozu izazvanu faktorom rasta ili lišavanjem seruma.[6][7]
- Simboli
10 | | 20 | | 30 | | 40 | | 50 |
MADEELEALR | | RQRLAELQAK | | HGDPGDAAQQ | | EAKHREAEMR | | NSILAQVLDQ |
SARARLSNLA | | LVKPEKTKAV | | ENYLIQMARY | | GQLSEKVSEQ | | GLIEILKKVS |
QQTEKTTTVK | | FNRRKVMDSD | | EDDDY |
Liu H, Wang Y, Zhang Y, Song Q, Di C, Chen G, Tang J, Ma D (Feb 1999). "TFAR19, a novel apoptosis-related gene cloned from human leukemia cell line TF-1, could enhance apoptosis of some tumor cells induced by growth factor withdrawal". Biochem Biophys Res Commun. 254 (1): 203–10. doi:10.1006/bbrc.1998.9893. PMID 9920759. Lisitsyn, N., Lisitsyn, N., Wigler, M. Cloning the differences between two complex genomes. Science 259: 946-951, 1993. [PubMed]: 8438152
Liu, H., Wang, Y., Zhang, Y., Song, Q., Di, C., Chen, G., Tang, J., Ma, D. TFAR19, a novel apoptosis-related gene cloned from human leukemia cell line TF-1, could enhance apoptosis of some tumor cells induced by growth factor withdrawal. Biochem. Biophys. Res. Commun. 254: 203-210, 1999. PubMed: 9920759.
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