LOC81691

Egzonukleaza NEF-sp, znana i kao LOC81691, je ljudski gen.^[5]

LOC81691
Identifikatori
Aliasi	REXO5
Vanjski ID-jevi	MGI: 1919402 HomoloGene: 12803 GeneCards: REXO5
Lokacija gena (čovjek) Hrom. Hromosom 16 (čovjek)[1] Bend 16p12.3 Početak 20,806,429 bp[1] Kraj 20,849,665 bp[1]
Lokacija gena (miš) Hrom. Hromosom 7 (miš)[2] Bend 7|7 F2 Početak 119,794,006 bp[2] Kraj 119,848,943 bp[2]
Obrazac RNK ekspresije Više referentnih podataka o ekspresiji
Ontologija gena Molekularna funkcija • nuclease activity • exonuclease activity • hydrolase activity • vezivanje sa RNK • nucleic acid binding Ćelijska komponenta • Jedarce • Egzosom • jedro Biološki proces • nucleic acid phosphodiester bond hydrolysis Izvori:Amigo / QuickGO
Ortolozi
Vrste	Čovjek	Miš
Entrez	81691	434234
Ensembl	ENSG00000005189	ENSMUSG00000030924
UniProt	Q96IC2	n/a
RefSeq (mRNK)	NM_001144924 NM_001199053 NM_030941	NM_001004187 NM_028129
RefSeq (bjelančevina)	NP_001138396 NP_001185982 NP_112203	n/a
Lokacija (UCSC)	Chr 16: 20.81 – 20.85 Mb	Chr 7: 119.79 – 119.85 Mb
PubMed pretraga	[3]	[4]
Wikipodaci
Pogledaj/uredi – čovjek Pogledaj/uredi – miš

Ribonukleaze kataliziraju sazrijevanje funkcionalnih RNK ili posreduju u razgradnji ćelijskih transkripata, aktivnosti koje su ključne za kontrolu ekspresije gena. Rekombinantni ljudski NEF-sp pokazuje dvovalentnu aktivnost ionski ovisne 3'→ 5' egzoribonukleaze. Ova aktivnost specifična je za jednolančane supstrate RNK i neovisna je o njihovoj dužini. Prisustvo 2'-O-metil modifikacije na 3 'kraju RNK supstrata je inhibitorno. Ektopijski izraženi NEF-sp lokalizira se u jedarni/jedarčani odjeljak u kulturama ćelija sisara i to ovisi o amino-terminalnom signalu jedarne lokalizacije. Konačno, miševi kojima nedostaje NEF-sp su održivi i pokazuju normalnu plodnost, što ukazuje na preklapanje funkcija s drugim nukleazama.^[6]

Aminokiselinska sekvenca

Dužina polipeptidnog lanca je 774 aminokiseline, a molekulska težina 86.886 Da.^[7]

Simboli

C: Cistein
D: Asparaginska kiselina
E: Glutaminska kiselina
F: Fenilalanin

G: Glicin
H: Histidin
I: Izoleucin
K: Lizin
L: Leucin
M: Metionin
N: Asparagin
P: Prolin
Q: Glutamin
R: Arginin
S: Serin
T: Treonin
V: Valin
W: Triptofan
Y: Tirozin

10		20		30		40		50
MEPEREGTER		HPRKVRESRQ		APNKLVGAAE		AMKAGWDLEE		SQPEAKKARL
STILFTDNCE		VTHDQLCELL		KYAVLGKSNV		PKPSWCQLFH		QNHLNNVVVF
VLQGMSQLHF		YRFYLEFGCL		RKAFRHKFRL		PPPSSDFLAD		VVGLQTEQRA
GDLPKTMEGP		LPSNAKAAIN		LQDDPIIQKY		GSKKVGLTRC		LLTKEEMRTF
HFPLQGFPDC		ENFLLTKCNG		SIADNSPLFG		LDCEMCLTSK		GRELTRISLV
AEGGCCVMDE		LVKPENKILD		YLTSFSGITK		KILNPVTTKL		KDVQRQLKAL
LPPDAVLVGH		SLDLDLRALK		MIHPYVIDTS		LLYVREQGRR		FKLKFLAKVI
LGKDIQCPDR		LGHDATEDAR		TILELARYFL		KHGPKKIAEL		NLEALANHQE
IQAAGQEPKN		TAEVLQHPNT		SVLECLDSVG		QKLLFLTRET		DAGELPSSRN
CQTIKCLSNK		EVLEQARVEI		PLFPFSIVQF		SFKAFSPVLT		EEMNKRMRIK
WTEISTVYAG		PFSKNCNLRA		LKRLFKSFGP		VQSMTFVLET		RQPHLCIQYE
VLEAAQLAIE		SLDGILVDGI		CIKVQRPVTE		LTLDCDTLVN		ELEGDSENQG
SIYLSGVSET		FKEQLLQEPR		LFLGLEAVIL		PKDLKSGKQK		KYCFLKFKSF
GSAQQALNIL		TGKDWKLKGR		HALTPRHLHA		WLRGLPPEST		RLPGLRVVPP
PFEQEALQTL		KLDHPKIAAW		RWSRKIGKLY		NSLCPGTLCL		ILLPGTKSTH
GSLSGLGLMG		IKEEEESAGP		GLCS

Reference

1. GRCh38: Ensembl release 89: ENSG00000005189 - Ensembl, maj 2017
2. GRCm38: Ensembl release 89: ENSMUSG00000030924 - Ensembl, maj 2017
3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
5. "Entrez Gene: LOC81691 exonuclease NEF-sp".
6. Sara Silva , David Homolka, Ramesh S Pillai (2017): Characterization of the mammalian RNA exonuclease 5/NEF-sp as a testis-specific nuclear 3' → 5' exoribonuclease. RNA, 23(9): 1385-1392
7. "UniProt, Q96IC2". Pristupljeno 7. 7. 2021.

Dopunska literatura

• Mehrle A, Rosenfelder H, Schupp I, et al. (2006). "The LIFEdb database in 2006". Nucleic Acids Res. 34 (Database issue): D415–8. doi:10.1093/nar/gkj139. PMC 1347501. PMID 16381901.
• Wiemann S, Arlt D, Huber W, et al. (2004). "From ORFeome to biology: a functional genomics pipeline". Genome Res. 14 (10B): 2136–44. doi:10.1101/gr.2576704. PMC 528930. PMID 15489336.
• Ota T, Suzuki Y, Nishikawa T, et al. (2004). "Complete sequencing and characterization of 21,243 full-length human cDNAs". Nat. Genet. 36 (1): 40–5. doi:10.1038/ng1285. PMID 14702039.
• Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932.
• Simpson JC, Wellenreuther R, Poustka A, et al. (2001). "Systematic subcellular localization of novel proteins identified by large-scale cDNA sequencing". EMBO Rep. 1 (3): 287–92. doi:10.1093/embo-reports/kvd058. PMC 1083732. PMID 11256614.
• Wiemann S, Weil B, Wellenreuther R, et al. (2001). "Toward a catalog of human genes and proteins: sequencing and analysis of 500 novel complete protein coding human cDNAs". Genome Res. 11 (3): 422–35. doi:10.1101/gr.GR1547R. PMC 311072. PMID 11230166.
• Hartley JL, Temple GF, Brasch MA (2001). "DNA cloning using in vitro site-specific recombination". Genome Res. 10 (11): 1788–95. doi:10.1101/gr.143000. PMC 310948. PMID 11076863.
• Loftus BJ, Kim UJ, Sneddon VP, et al. (1999). "Genome duplications and other features in 12 Mb of DNA sequence from human chromosome 16p and 16q". Genomics. 60 (3): 295–308. doi:10.1006/geno.1999.5927. PMID 10493829.