Protein 1 sa domenom fumarilacetoacetat-hidrolaze, znan i kao protein FLJ36880, je enzim koji je kod ljudi kodiran genom FAHD1 na hromosomu 16.[3]
Kratke činjenice Dostupne strukture, PDB ...
Zatvori
Gen FAHD1 kodira protein od 24 kDa koji je lokaliziran u mitohondrijama i pripada proteinskoj porodici fumarilacetoacetat-hidrolaza.[4] Struktura FAHD1 razriješena je upotrebom rendgenske kristalografije pri rezoluciji od 2,2 Å. Ukupna struktura slična je C-terminalnom domenu bifunkcionalnog enzima HpcE iz Escherichia coli C, fumarilacetoacetat hidrolaze iz Mus musculus i YcgM (Apc5008) iz E. coli 1262.[4] Izgleda da je za njegovu katalitsku aktivnost važan niz konzerviranih aminokiselina, uključujući Asp-102 i Arg-106 FAHD1.[5]
Dužina polipeptidnog lanca je 224 aminokiseline, a molekulska težina 24.843 Da.[6]
10 | | 20 | | 30 | | 40 | | 50 |
MGIMAASRPL | | SRFWEWGKNI | | VCVGRNYADH | | VREMRSAVLS | | EPVLFLKPST |
AYAPEGSPIL | | MPAYTRNLHH | | ELELGVVMGK | | RCRAVPEAAA | | MDYVGGYALC |
LDMTARDVQD | | ECKKKGLPWT | | LAKSFTASCP | | VSAFVPKEKI | | PDPHKLKLWL |
KVNGELRQEG | | ETSSMIFSIP | | YIISYVSKII | | TLEEGDIILT | | GTPKGVGPVK |
ENDEIEAGIH | | GLVSMTFKVE | | KPEY |
- Simboli
Pokazalo se da u eukariota protein FAHD1 funkcionira kao oksaloacetatna dekarboksilaza.[7] Protein FAHD1 vjerovatno također funkcionira kao acilpiruvaza, a pokazalo se da katalizira hidrolizu acetilpiruvata i fumarilpiruvata u in vitro eksperimentima.[4] Za maksimalnu aktivnost enzima bio je potreban Mg(2+).[5]
- Hartley JL, Temple GF, Brasch MA (2001). "DNA cloning using in vitro site-specific recombination". Genome Res. 10 (11): 1788–95. doi:10.1101/gr.143000. PMC 310948. PMID 11076863.
- Daniels RJ, Peden JF, Lloyd C, et al. (2001). "Sequence, structure and pathology of the fully annotated terminal 2 Mb of the short arm of human chromosome 16". Hum. Mol. Genet. 10 (4): 339–52. doi:10.1093/hmg/10.4.339. PMID 11157797.
- Wiemann S, Weil B, Wellenreuther R, et al. (2001). "Toward a catalog of human genes and proteins: sequencing and analysis of 500 novel complete protein coding human cDNAs". Genome Res. 11 (3): 422–35. doi:10.1101/gr.GR1547R. PMC 311072. PMID 11230166.
- Simpson JC, Wellenreuther R, Poustka A, et al. (2001). "Systematic subcellular localization of novel proteins identified by large-scale cDNA sequencing". EMBO Rep. 1 (3): 287–92. doi:10.1093/embo-reports/kvd058. PMC 1083732. PMID 11256614.
- Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932.
- Ota T, Suzuki Y, Nishikawa T, et al. (2004). "Complete sequencing and characterization of 21,243 full-length human cDNAs". Nat. Genet. 36 (1): 40–5. doi:10.1038/ng1285. PMID 14702039.
- Lehner B, Sanderson CM (2004). "A protein interaction framework for human mRNA degradation". Genome Res. 14 (7): 1315–23. doi:10.1101/gr.2122004. PMC 442147. PMID 15231747.
- Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC 528928. PMID 15489334.
- Wiemann S, Arlt D, Huber W, et al. (2004). "From ORFeome to biology: a functional genomics pipeline". Genome Res. 14 (10B): 2136–44. doi:10.1101/gr.2576704. PMC 528930. PMID 15489336.
- Manjasetty BA, Niesen FH, Delbrück H, et al. (2005). "X-ray structure of fumarylacetoacetate hydrolase family member Homo sapiens FLJ36880". Biol. Chem. 385 (10): 935–42. doi:10.1515/BC.2004.122. PMID 15551868. S2CID 16759973.
- Mehrle A, Rosenfelder H, Schupp I, et al. (2006). "The LIFEdb database in 2006". Nucleic Acids Res. 34 (Database issue): D415–8. doi:10.1093/nar/gkj139. PMC 1347501. PMID 16381901.