Plazmamembranska kalcij-transportirajuća ATPaza 4 jest enzim koji je kod ljudi kodiran genom ATP2B4 sa hromosoma 1.[5][6]
Kratke činjenice Dostupne strukture, PDB ...
ATP2B4 |
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Identifikatori |
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Aliasi | ATP2B4 |
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Vanjski ID-jevi | OMIM: 108732 MGI: 88111 HomoloGene: 48034 GeneCards: ATP2B4 |
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Ontologija gena |
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Molekularna funkcija | • nucleotide binding • PDZ domain binding • sodium channel regulator activity • nitric-oxide synthase binding • scaffold protein binding • vezivanje iona metala • calmodulin binding • nitric-oxide synthase inhibitor activity • GO:0001948, GO:0016582 vezivanje za proteine • protein phosphatase 2B binding • ATP binding • hydrolase activity • P-type calcium transporter activity • protein kinase binding
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Ćelijska komponenta | • integral component of membrane • projekcija ćelije • membrana • intracellular membrane-bounded organelle • T-tubule • ćelijska membrana • integral component of plasma membrane • sperm flagellum • sperm principal piece • basolateral plasma membrane • Z disc • Kaveole • neuron projection • Sarkolema • Treplja • motile cilium • GO:0009327 makromolekulani kompleks • glutamatergic synapse • integral component of presynaptic active zone membrane
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Biološki proces | • negative regulation of nitric oxide mediated signal transduction • flagellated sperm motility • regulation of cardiac conduction • negative regulation of nitric-oxide synthase activity • negative regulation of calcineurin-NFAT signaling cascade • negative regulation of citrulline biosynthetic process • negative regulation of peptidyl-cysteine S-nitrosylation • GO:0044324, GO:0003256, GO:1901213, GO:0046019, GO:0046020, GO:1900094, GO:0061216, GO:0060994, GO:1902064, GO:0003258, GO:0072212 regulation of transcription by RNA polymerase II • calcium ion import across plasma membrane • response to hydrostatic pressure • cellular calcium ion homeostasis • ion transport • positive regulation of peptidyl-serine phosphorylation • cellular response to epinephrine stimulus • ion transmembrane transport • negative regulation of nitric oxide biosynthetic process • calcium ion transmembrane transport • GO:0060401 calcium ion transmembrane import into cytosol • Spermatogeneza • positive regulation of cAMP-dependent protein kinase activity • regulation of cell cycle G1/S phase transition • regulation of sodium ion transmembrane transport • calcium ion export • hippocampus development • GO:0100045 negative regulation of arginine catabolic process • neural retina development • negative regulation of the force of heart contraction • calcium ion transport • negative regulation of cardiac muscle hypertrophy in response to stress • GO:0015915 transport • regulation of cytosolic calcium ion concentration • negative regulation of adenylate cyclase-activating adrenergic receptor signaling pathway involved in heart process • urinary bladder smooth muscle contraction • cellular response to acetylcholine
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Izvori:Amigo / QuickGO |
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Ortolozi |
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Vrste | Čovjek | Miš |
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Entrez | | |
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Ensembl | | |
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UniProt | | |
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RefSeq (mRNK) | | |
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RefSeq (bjelančevina) | | |
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Lokacija (UCSC) | Chr 1: 203.63 – 203.74 Mb | Chr 1: 133.63 – 133.73 Mb |
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PubMed pretraga | [3] | [4] |
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Wikipodaci |
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Zatvori
Dužina polipeptidnog lanca je 1.241 aminokiselina, a molekulska težina 137.920 Da.[7]
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MTNPSDRVLP | | ANSMAESREG | | DFGCTVMELR | | KLMELRSRDA | | LTQINVHYGG |
VQNLCSRLKT | | SPVEGLSGNP | | ADLEKRRQVF | | GHNVIPPKKP | | KTFLELVWEA |
LQDVTLIILE | | IAAIISLVLS | | FYRPAGEENE | | LCGQVATTPE | | DENEAQAGWI |
EGAAILFSVI | | IVVLVTAFND | | WSKEKQFRGL | | QCRIEQEQKF | | SIIRNGQLIQ |
LPVAEIVVGD | | IAQVKYGDLL | | PADGILIQGN | | DLKIDESSLT | | GESDHVKKSL |
DKDPMLLSGT | | HVMEGSGRMV | | VTAVGVNSQT | | GIILTLLGVN | | EDDEGEKKKK |
GKKQGVPENR | | NKAKTQDGVA | | LEIQPLNSQE | | GIDNEEKDKK | | AVKVPKKEKS |
VLQGKLTRLA | | VQIGKAGLLM | | SALTVFILIL | | YFVIDNFVIN | | RRPWLPECTP |
IYIQYFVKFF | | IIGITVLVVA | | VPEGLPLAVT | | ISLAYSVKKM | | MKDNNLVRHL |
DACETMGNAT | | AICSDKTGTL | | TMNRMTVVQA | | YIGGIHYRQI | | PSPDVFLPKV |
LDLIVNGISI | | NSAYTSKILP | | PEKEGGLPRQ | | VGNKTECALL | | GFVTDLKQDY |
QAVRNEVPEE | | KLYKVYTFNS | | VRKSMSTVIR | | NPNGGFRMYS | | KGASEIILRK |
CNRILDRKGE | | AVPFKNKDRD | | DMVRTVIEPM | | ACDGLRTICI | | AYRDFDDTEP |
SWDNENEILT | | ELTCIAVVGI | | EDPVRPEVPD | | AIAKCKQAGI | | TVRMVTGDNI |
NTARAIATKC | | GILTPGDDFL | | CLEGKEFNRL | | IRNEKGEVEQ | | EKLDKIWPKL |
RVLARSSPTD | | KHTLVKGIID | | STVGEHRQVV | | AVTGDGTNDG | | PALKKADVGF |
AMGIAGTDVA | | KEASDIILTD | | DNFTSIVKAV | | MWGRNVYDSI | | SKFLQFQLTV |
NVVAVIVAFT | | GACITQDSPL | | KAVQMLWVNL | | IMDTFASLAL | | ATEPPTESLL |
KRRPYGRNKP | | LISRTMMKNI | | LGHAFYQLIV | | IFILVFAGEK | | FFDIDSGRKA |
PLHSPPSQHY | | TIVFNTFVLM | | QLFNEINSRK | | IHGEKNVFSG | | IYRNIIFCSV |
VLGTFICQIF | | IVEFGGKPFS | | CTSLSLSQWL | | WCLFIGIGEL | | LWGQFISAIP |
TRSLKFLKEA | | GHGTTKEEIT | | KDAEGLDEID | | HAEMELRRGQ | | ILWFRGLNRI |
QTQIDVINTF | | QTGASFKGVL | | RRQNMGQHLD | | VKLVPSSSYI | | KVVKAFHSSL |
HESIQKPYNQ | | KSIHSFMTHP | | EFAIEEELPR | | TPLLDEEEEE | | NPDKASKFGT |
RVLLLDGEVT | | PYANTNNNAV | | DCNQVQLPQS | | DSSLQSLETS | | V |
Protein kodiran ovim genom pripada familiji P-tipa primarnih transportnih iona ATPaza, koje karakterizira formiranje intermedijera aspartil-fosfata tokom reakcijskog ciklusa. Ovi enzimi uklanjaju dvovalentne ione kalcija iz eukariotskih ćelija protiv vrlo velikih gradijenata koncentracije i imaju ključnu ulogu u unutarćelijskoj homeostazi kalcija. Izoforme plazmamembranske kalcijske ATPaze sisara kodirane su sa najmanje četiri odvojena gena, a raznolikost ovih enzima je dodatno povećana alternativnom preradom transkripta. Ekspresija različitih izoformi i varijanti prerade regulirana je na način specifičan za razvoj, tkivo i tip sčelija, što sugerira da su ove pumpe funkcionalno prilagođene fiziološkim potrebama određenih ćelija i tkiva. Ovaj gen kodira izoformu 4 kalcijeve plazmamembranske ATPaze. Identificirane su alternativno prerađene varijante transkripta koje kodiraju različite izoforme.[6]
Pokazalo se da ATP2B4 ima interakcije sa CASK.[8]
Olson S, Wang MG, Carafoli E, Strehler EE, McBride OW (april 1991). "Localization of two genes encoding plasma membrane Ca2(+)-transporting ATPases to human chromosomes 1q25-32 and 12q21-23". Genomics. 9 (4): 629–41. doi:10.1016/0888-7543(91)90356-J. PMID 1674727.
- Møller JV, Juul B, le Maire M (maj 1996). "Structural organization, ion transport, and energy transduction of P-type ATPases". Biochimica et Biophysica Acta (BBA) - Reviews on Biomembranes. 1286 (1): 1–51. doi:10.1016/0304-4157(95)00017-8. PMID 8634322.
- Strehler EE, Zacharias DA (januar 2001). "Role of alternative splicing in generating isoform diversity among plasma membrane calcium pumps". Physiological Reviews. 81 (1): 21–50. doi:10.1152/physrev.2001.81.1.21. PMID 11152753.
- Strehler EE, Treiman M (maj 2004). "Calcium pumps of plasma membrane and cell interior". Current Molecular Medicine. 4 (3): 323–35. doi:10.2174/1566524043360735. PMID 15101689.
- Heim R, Hug M, Iwata T, Strehler EE, Carafoli E (april 1992). "Microdiversity of human-plasma-membrane calcium-pump isoform 2 generated by alternative RNA splicing in the N-terminal coding region". European Journal of Biochemistry. 205 (1): 333–40. doi:10.1111/j.1432-1033.1992.tb16784.x. PMID 1313367.
- Brandt P, Neve RL, Kammesheidt A, Rhoads RE, Vanaman TC (mart 1992). "Analysis of the tissue-specific distribution of mRNAs encoding the plasma membrane calcium-pumping ATPases and characterization of an alternately spliced form of PMCA4 at the cDNA and genomic levels". The Journal of Biological Chemistry. 267 (7): 4376–85. doi:10.1016/S0021-9258(18)42846-3. PMID 1531651.
- Strehler EE, James P, Fischer R, Heim R, Vorherr T, Filoteo AG, Penniston JT, Carafoli E (februar 1990). "Peptide sequence analysis and molecular cloning reveal two calcium pump isoforms in the human erythrocyte membrane". The Journal of Biological Chemistry. 265 (5): 2835–42. doi:10.1016/S0021-9258(19)39877-1. PMID 2137451.
- James P, Maeda M, Fischer R, Verma AK, Krebs J, Penniston JT, Carafoli E (februar 1988). "Identification and primary structure of a calmodulin binding domain of the Ca2+ pump of human erythrocytes". The Journal of Biological Chemistry. 263 (6): 2905–10. doi:10.1016/S0021-9258(18)69154-9. PMID 2963820.
- Brandt P, Zurini M, Neve RL, Rhoads RE, Vanaman TC (maj 1988). "A C-terminal, calmodulin-like regulatory domain from the plasma membrane Ca2+-pumping ATPase". Proceedings of the National Academy of Sciences of the United States of America. 85 (9): 2914–8. Bibcode:1988PNAS...85.2914B. doi:10.1073/pnas.85.9.2914. PMC 280113. PMID 2966397.
- Howard A, Barley NF, Legon S, Walters JR (oktobar 1994). "Plasma-membrane calcium-pump isoforms in human and rat liver". The Biochemical Journal. 303 ( Pt 1) (Pt 1): 275–9. doi:10.1042/bj3030275. PMC 1137587. PMID 7945253.
- Stauffer TP, Hilfiker H, Carafoli E, Strehler EE (decembar 1994). "Quantitative analysis of alternative splicing options of human plasma membrane calcium pump genes". The Journal of Biological Chemistry. 269 (50): 32022. doi:10.1016/S0021-9258(18)31797-6. PMID 7989379.
- Stauffer TP, Hilfiker H, Carafoli E, Strehler EE (decembar 1993). "Quantitative analysis of alternative splicing options of human plasma membrane calcium pump genes". The Journal of Biological Chemistry. 268 (34): 25993–6003. doi:10.1016/S0021-9258(19)74484-6. PMID 8245032.
- Váradi A, Molnár E, Ashcroft SJ (mart 1996). "A unique combination of plasma membrane Ca2+-ATPase isoforms is expressed in islets of Langerhans and pancreatic beta-cell lines". The Biochemical Journal. 314 ( Pt 2) (Pt 2): 663–9. doi:10.1042/bj3140663. PMC 1217098. PMID 8670083.
- Santiago-García J, Mas-Oliva J, Saavedra D, Zarain-Herzberg A (februar 1996). "Analysis of mRNA expression and cloning of a novel plasma membrane Ca(2+)-ATPase splice variant in human heart". Molecular and Cellular Biochemistry. 155 (2): 173–82. doi:10.1007/BF00229314. PMID 8700162. S2CID 33539531.
- Zacharias DA, DeMarco SJ, Strehler EE (april 1997). "mRNA expression of the four isoforms of the human plasma membrane Ca(2+)-ATPase in the human hippocampus". Brain Research. Molecular Brain Research. 45 (1): 173–6. doi:10.1016/S0169-328X(97)00009-0. PMID 9105688.
- Dean WL, Chen D, Brandt PC, Vanaman TC (juni 1997). "Regulation of platelet plasma membrane Ca2+-ATPase by cAMP-dependent and tyrosine phosphorylation". The Journal of Biological Chemistry. 272 (24): 15113–9. doi:10.1074/jbc.272.24.15113. PMID 9182531.
- Kim E, DeMarco SJ, Marfatia SM, Chishti AH, Sheng M, Strehler EE (januar 1998). "Plasma membrane Ca2+ ATPase isoform 4b binds to membrane-associated guanylate kinase (MAGUK) proteins via their PDZ (PSD-95/Dlg/ZO-1) domains". The Journal of Biological Chemistry. 273 (3): 1591–5. doi:10.1074/jbc.273.3.1591. PMID 9430700.
- Guerini D, García-Martin E, Gerber A, Volbracht C, Leist M, Merino CG, Carafoli E (januar 1999). "The expression of plasma membrane Ca2+ pump isoforms in cerebellar granule neurons is modulated by Ca2+". The Journal of Biological Chemistry. 274 (3): 1667–76. doi:10.1074/jbc.274.3.1667. PMID 9880546.
- Rosado JA, Sage SO (juni 2000). "Regulation of plasma membrane Ca2+-ATPase by small GTPases and phosphoinositides in human platelets". The Journal of Biological Chemistry. 275 (26): 19529–35. doi:10.1074/jbc.M001319200. PMID 10748016.