核仁转录因子1(Nucleolar transcription factor 1)又叫上游结合转录因子(Upstream binding transcription factor,缩写UBTF)或上游结合因子1(Upstream binding factor 1,缩写UBF1)是一种由人类基因UBTF 所编码的蛋白质[1][2]。
事实速览 Upstream binding transcription factor, RNA polymerase I RNA聚合酶I上游结合因子, 有效结构 ...
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UBTF是18S、5.8S和28S的核糖体RNA的转录所必须的转录因子,和SL1因子(一个四聚体,包括一个TATA结合蛋白和3个转录辅助因子TAF1)共同起作用。在人体中存在94和97kD的两种UBF多肽链。UBF是一种核仁磷酸化蛋白(nucleolar phosphoprotein),同时具有DNA结合结构域和反式激活结构域,通过HMG盒结合到人rRNA启动子的核心与上游部位。[2]
UBTF已知能与酪蛋白激酶2α亚基[3]、TAF1C[4][5]、视网膜母细胞瘤蛋白[6]和TAF1[7]进行交互作用。
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Hannan, K M; Hannan R D, Smith S D, Jefferson L S, Lun M, Rothblum L I. Rb and p130 regulate RNA polymerase I transcription: Rb disrupts the interaction between UBF and SL-1. Oncogene (ENGLAND). October 2000, 19 (43): 4988–99. ISSN 0950-9232. PMID 11042686. doi:10.1038/sj.onc.1203875.
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- Jantzen HM, Admon A, Bell SP, Tjian R. Nucleolar transcription factor hUBF contains a DNA-binding motif with homology to HMG proteins. Nature. 1990, 344 (6269): 830–6. PMID 2330041. doi:10.1038/344830a0.
- Bell SP, Learned RM, Jantzen HM, Tjian R. Functional cooperativity between transcription factors UBF1 and SL1 mediates human ribosomal RNA synthesis. Science. 1988, 241 (4870): 1192–7. PMID 3413483. doi:10.1126/science.3413483.
- Voit R, Kuhn A, Sander EE, Grummt I. Activation of mammalian ribosomal gene transcription requires phosphorylation of the nucleolar transcription factor UBF. Nucleic Acids Res. 1995, 23 (14): 2593–9. PMC 307079 . PMID 7651819. doi:10.1093/nar/23.14.2593.
- Hempel WM; Cavanaugh AH; Hannan RD; et al. The species-specific RNA polymerase I transcription factor SL-1 binds to upstream binding factor. Mol. Cell. Biol. 1996, 16 (2): 557–63. PMC 231034 . PMID 8552083.
- Hanada K; Song CZ; Yamamoto K; et al. RNA polymerase I associated factor 53 binds to the nucleolar transcription factor UBF and functions in specific rDNA transcription. EMBO J. 1996, 15 (9): 2217–26. PMC 450146 . PMID 8641287.
- Pluta AF, Earnshaw WC. Specific interaction between human kinetochore protein CENP-C and a nucleolar transcriptional regulator. J. Biol. Chem. 1996, 271 (31): 18767–74. PMID 8702533. doi:10.1074/jbc.271.31.18767.
- Whitehead CM, Winkfein RJ, Fritzler MJ, Rattner JB. ASE-1: a novel protein of the fibrillar centres of the nucleolus and nucleolus organizer region of mitotic chromosomes. Chromosoma. 1998, 106 (8): 493–502. PMID 9426281. doi:10.1007/s004120050271.
- Voit R, Hoffmann M, Grummt I. Phosphorylation by G1-specific cdk-cyclin complexes activates the nucleolar transcription factor UBF. EMBO J. 1999, 18 (7): 1891–9. PMC 1171274 . PMID 10202152. doi:10.1093/emboj/18.7.1891.
- Liu CJ, Wang H, Lengyel P. The interferon-inducible nucleolar p204 protein binds the ribosomal RNA-specific UBF1 transcription factor and inhibits ribosomal RNA transcription. EMBO J. 1999, 18 (10): 2845–54. PMC 1171365 . PMID 10329630. doi:10.1093/emboj/18.10.2845.
- Hannan KM; Hannan RD; Smith SD; et al. Rb and p130 regulate RNA polymerase I transcription: Rb disrupts the interaction between UBF and SL-1. Oncogene. 2000, 19 (43): 4988–99. PMID 11042686. doi:10.1038/sj.onc.1203875.
- Kalousek I, Krízková P. Lymphocyte mitogenic transformation is accompanied by phosphorylation of the nucleolar transcription factor UBF. Cell. Mol. Biol. (Noisy-le-grand). 2001, 46 (7): 1163–71. PMID 11075946.
- Pelletier G; Stefanovsky VY; Faubladier M; et al. Competitive recruitment of CBP and Rb-HDAC regulates UBF acetylation and ribosomal transcription. Mol. Cell. 2002, 6 (5): 1059–66. PMID 11106745. doi:10.1016/S1097-2765(00)00104-0.
- Miller G; Panov KI; Friedrich JK; et al. hRRN3 is essential in the SL1-mediated recruitment of RNA Polymerase I to rRNA gene promoters. EMBO J. 2001, 20 (6): 1373–82. PMC 145519 . PMID 11250903. doi:10.1093/emboj/20.6.1373.
- Seither P, Iben S, Thiry M, Grummt I. PAF67, a novel protein that is associated with the initiation-competent form of RNA polymerase I. Biol. Chem. 2002, 382 (8): 1163–70. PMID 11592397. doi:10.1515/BC.2001.146.
- Voit R, Grummt I. Phosphorylation of UBF at serine 388 is required for interaction with RNA polymerase I and activation of rDNA transcription. Proc. Natl. Acad. Sci. U.S.A. 2002, 98 (24): 13631–6. PMC 61092 . PMID 11698641. doi:10.1073/pnas.231071698.
- Stefanovsky VY; Pelletier G; Hannan R; et al. An immediate response of ribosomal transcription to growth factor stimulation in mammals is mediated by ERK phosphorylation of UBF. Mol. Cell. 2002, 8 (5): 1063–73. PMID 11741541. doi:10.1016/S1097-2765(01)00384-7.
- Andersen JS; Lyon CE; Fox AH; et al. Directed proteomic analysis of the human nucleolus. Curr. Biol. 2002, 12 (1): 1–11. PMID 11790298. doi:10.1016/S0960-9822(01)00650-9.
- Dühr S; Torres-Montaner A; Astola A; et al. Molecular analysis of the 5' region of human ribosomal transcription factor UBF. DNA Seq. 2002, 12 (4): 267–72. PMID 11916260. doi:10.3109/10425170109025001.