KG-1

KG-1是一種假二倍體人類急性骨髓性白血病細胞系，最初分離自一名59歲患有紅白血病（erythroleukemia）而後來又發展成急性骨髓性白血病的白人男性患者的骨髓。KG-1細胞在8號染色體的長臂上具有部分「hexasomy」，這或能夠解釋KG-1細胞中AML1-ETO融合基因的形成速率明顯更高的原因^[1]。KG-1細胞在形態上類似急性骨髓性白血病細胞，表現為明顯的多形化，骨髓母細胞（英語：Myeloblast）和骨髓細胞佔優勢，同時又有少量的成熟粒細胞，以及微量的巨噬細胞和嗜酸性球。

KG-1細胞及分化程度較低的變異亞系KG-1a細胞目前常用於科學研究上。KG-1細胞可以研究與白血病相關的FGFR1（英語：Fibroblast growth factor receptor 1）融合物，以及是分析針對FGFR1融合物的小分子抑製劑的體外模型^[2]。此外，KG-1細胞被認為是人類樹突狀細胞祖細胞的模型。目前已經知道介白素-18可以誘導這些細胞向樹突狀表型生長，並且在該模型中能夠介導γ-干擾素的產生^[3]。KG-1細胞的變異亞系KG-1a細胞則失去了髓樣特徵，獲得新的核型標記，並且具有三個與未成熟T細胞相關的特徵：從種系基因轉錄的T細胞受體 β mRNA的低水平表達、T3 δ mRNA和細胞內T3蛋白的高水平表達，以及CD7/gp40 T細胞相關膜抗原的表達^[4]。目前已知KG-1a細胞可以用於研究細胞對集落刺激因子的反應，以及研究白血病細胞中的抗原表達^[5]。

[1]
Mrózek, K; Tanner, SM; Heinonen, K; Bloomfield, CD. Molecular cytogenetic characterization of the KG-1 and KG-1a acute myeloid leukemia cell lines by use of spectral karyotyping and fluorescence in situ hybridization.. Genes, chromosomes & cancer. 2003-11, 38 (3): 249–52 [2020-02-19]. PMID 14506699. doi:10.1002/gcc.10274.^{[永久失效連結]}
[2]
Gu, TL; Goss, VL; Reeves, C; Popova, L; Nardone, J; Macneill, J; Walters, DK; Wang, Y; Rush, J; Comb, MJ; Druker, BJ; Polakiewicz, RD. Phosphotyrosine profiling identifies the KG-1 cell line as a model for the study of FGFR1 fusions in acute myeloid leukemia.. Blood. 2006-12-15, 108 (13): 4202–4 [2020-02-19]. PMID 16946300. doi:10.1182/blood-2006-06-026666.^{[永久失效連結]}
[3]
Bachmann, M; Dragoi, C; Poleganov, MA; Pfeilschifter, J; Mühl, H. Interleukin-18 directly activates T-bet expression and function via p38 mitogen-activated protein kinase and nuclear factor-kappaB in acute myeloid leukemia-derived predendritic KG-1 cells.. Molecular cancer therapeutics. 2007-02, 6 (2): 723–31 [2020-02-19]. PMID 17308068. doi:10.1158/1535-7163.MCT-06-0505.^{[永久失效連結]}
[4]
Furley, AJ; Reeves, BR; Mizutani, S; Altass, LJ; Watt, SM; Jacob, MC; van den Elsen, P; Terhorst, C; Greaves, MF. Divergent molecular phenotypes of KG1 and KG1a myeloid cell lines.. Blood. 1986-11, 68 (5): 1101–7 [2020-02-19]. PMID 3094604.^{[永久失效連結]}
[5]
Koeffler, HP; Billing, R; Lusis, AJ; Sparkes, R; Golde, DW. An undifferentiated variant derived from the human acute myelogenous leukemia cell line (KG-1).. Blood. 1980-08, 56 (2): 265–73 [2020-02-19]. PMID 6967340.^{[永久失效連結]}

Cellosaurus entry for KG-1 （頁面存檔備份，存於互聯網檔案館）

[1] [1]
Mrózek, K; Tanner, SM; Heinonen, K; Bloomfield, CD. Molecular cytogenetic characterization of the KG-1 and KG-1a acute myeloid leukemia cell lines by use of spectral karyotyping and fluorescence in situ hybridization.. Genes, chromosomes & cancer. 2003-11, 38 (3): 249–52 [2020-02-19]. PMID 14506699. doi:10.1002/gcc.10274.^{[永久失效連結]}

[2] [2]
Gu, TL; Goss, VL; Reeves, C; Popova, L; Nardone, J; Macneill, J; Walters, DK; Wang, Y; Rush, J; Comb, MJ; Druker, BJ; Polakiewicz, RD. Phosphotyrosine profiling identifies the KG-1 cell line as a model for the study of FGFR1 fusions in acute myeloid leukemia.. Blood. 2006-12-15, 108 (13): 4202–4 [2020-02-19]. PMID 16946300. doi:10.1182/blood-2006-06-026666.^{[永久失效連結]}

[3] [3]
Bachmann, M; Dragoi, C; Poleganov, MA; Pfeilschifter, J; Mühl, H. Interleukin-18 directly activates T-bet expression and function via p38 mitogen-activated protein kinase and nuclear factor-kappaB in acute myeloid leukemia-derived predendritic KG-1 cells.. Molecular cancer therapeutics. 2007-02, 6 (2): 723–31 [2020-02-19]. PMID 17308068. doi:10.1158/1535-7163.MCT-06-0505.^{[永久失效連結]}

[4] [4]
Furley, AJ; Reeves, BR; Mizutani, S; Altass, LJ; Watt, SM; Jacob, MC; van den Elsen, P; Terhorst, C; Greaves, MF. Divergent molecular phenotypes of KG1 and KG1a myeloid cell lines.. Blood. 1986-11, 68 (5): 1101–7 [2020-02-19]. PMID 3094604.^{[永久失效連結]}

[5] [5]
Koeffler, HP; Billing, R; Lusis, AJ; Sparkes, R; Golde, DW. An undifferentiated variant derived from the human acute myelogenous leukemia cell line (KG-1).. Blood. 1980-08, 56 (2): 265–73 [2020-02-19]. PMID 6967340.^{[永久失效連結]}

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[5]

KG-1

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KG-1

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研究用途

參考資料

外部連結