CAKI-1

CAKI-1 (亦称ATCC HTB-46) 是人类肾透明细胞癌皮肤转移细胞系^[1]，最初是分离自一名49岁的白人男性肾癌患者^[2]。

Caki-1细胞中高表达的基因345个，而低表达的基因则有217个^[3]。CAKI-1细胞的染色体倍性在三倍体范围内，并且不存在Y染色体，因为Y染色体的缺失在雄性肿瘤细胞系中并不罕见。除染色体N9和N19以外，所有的染色体都拥有2或3个拷贝。Caki-1细胞有许多微绒毛、发育充分的高尔基体与内质网、细小的线粒体及少许微丝，目前并没有在细胞内发现任何病毒颗粒。

有学者提议将其作为近端肾小管上皮组织的模型，因为细胞在培养过程中，可以形成具有功能性及高度分化的肾组织形态、生理及生化特征的极化层 (polarized layer) ^[4]。有研究指出Caki-1是转移性肾亮细胞癌（英语：Clear cell renal cell carcinoma）的常用模型，当其携带着野生型vhl抑癌基因时，它就能在无胸腺裸鼠中产生清晰可见的肿瘤^[5]。这些细胞高表达血管内皮生长因子^[6]（特别是在氧气浓度低的情况^[7]）的特征就是肾亮细胞癌（英语：Clear cell renal cell carcinoma）的标志。

[1]
Breuksch, I; Prosinger, F; Baehr, F; Engelhardt, FP; Bauer, HK; Thüroff, JW; Heimes, AS; Hasenburg, A; Prawitt, D; Brenner, W. Integrin α5 triggers the metastatic potential in renal cell carcinoma.. Oncotarget. 2017-12-08, 8 (64): 107530–107542 [2019-12-09]. PMID 29296184. doi:10.18632/oncotarget.22501.
[2]
Fogh, J; Wright, WC; Loveless, JD. Absence of HeLa cell contamination in 169 cell lines derived from human tumors.. Journal of the National Cancer Institute. 1977-02, 58 (2): 209–14 [2019-12-09]. PMID 833871. doi:10.1093/jnci/58.2.209.
[3]
Hehuan Zhu. Bioinformatics analysis of differentially expressed genes in clear cell renal cell carcinoma Caki-1 cell line. Chinese Journal of Cell and Stem Cell. 2018-11, 8 (2): 80–7 [2019-12-09]. doi:10.3877//cma.j.issn.2095-1221.2018.02.003. （原始内容存档于2019-12-09）.
[4]
Glube, N; Giessl, A; Wolfrum, U; Langguth, P. Caki-1 cells represent an in vitro model system for studying the human proximal tubule epithelium.. Nephron. Experimental nephrology. 2007, 107 (2): e47–56 [2019-12-09]. PMID 17804913. doi:10.1159/000107804.
[5]
Lichner, Z; Saleh, C; Subramaniam, V; Seivwright, A; Prud'homme, GJ; Yousef, GM. miR-17 inhibition enhances the formation of kidney cancer spheres with stem cell/ tumor initiating cell properties.. Oncotarget. 2015-03-20, 6 (8): 5567–81 [2019-12-09]. PMID 25011053. doi:10.18632/oncotarget.1901.
[6]
Liu, YH; Lin, CY; Lin, WC; Tang, SW; Lai, MK; Lin, JY. Up-regulation of vascular endothelial growth factor-D expression in clear cell renal cell carcinoma by CD74: a critical role in cancer cell tumorigenesis.. Journal of immunology (Baltimore, Md. : 1950). 2008-11-01, 181 (9): 6584–94 [2019-12-09]. PMID 18941249. doi:10.4049/jimmunol.181.9.6584.
[7]
Miyake, M; Goodison, S; Lawton, A; Zhang, G; Gomes-Giacoia, E; Rosser, CJ. Erythropoietin is a JAK2 and ERK1/2 effector that can promote renal tumor cell proliferation under hypoxic conditions.. Journal of hematology & oncology. 2013-09-03, 6: 65 [2019-12-09]. PMID 24004818. doi:10.1186/1756-8722-6-65.

Cellosaurus entry for CAKI-1（页面存档备份，存于互联网档案馆）

[1] [1]
Breuksch, I; Prosinger, F; Baehr, F; Engelhardt, FP; Bauer, HK; Thüroff, JW; Heimes, AS; Hasenburg, A; Prawitt, D; Brenner, W. Integrin α5 triggers the metastatic potential in renal cell carcinoma.. Oncotarget. 2017-12-08, 8 (64): 107530–107542 [2019-12-09]. PMID 29296184. doi:10.18632/oncotarget.22501.

[2] [2]
Fogh, J; Wright, WC; Loveless, JD. Absence of HeLa cell contamination in 169 cell lines derived from human tumors.. Journal of the National Cancer Institute. 1977-02, 58 (2): 209–14 [2019-12-09]. PMID 833871. doi:10.1093/jnci/58.2.209.

[3] [3]
Hehuan Zhu. Bioinformatics analysis of differentially expressed genes in clear cell renal cell carcinoma Caki-1 cell line. Chinese Journal of Cell and Stem Cell. 2018-11, 8 (2): 80–7 [2019-12-09]. doi:10.3877//cma.j.issn.2095-1221.2018.02.003. （原始内容存档于2019-12-09）.

[4] [4]
Glube, N; Giessl, A; Wolfrum, U; Langguth, P. Caki-1 cells represent an in vitro model system for studying the human proximal tubule epithelium.. Nephron. Experimental nephrology. 2007, 107 (2): e47–56 [2019-12-09]. PMID 17804913. doi:10.1159/000107804.

[5] [5]
Lichner, Z; Saleh, C; Subramaniam, V; Seivwright, A; Prud'homme, GJ; Yousef, GM. miR-17 inhibition enhances the formation of kidney cancer spheres with stem cell/ tumor initiating cell properties.. Oncotarget. 2015-03-20, 6 (8): 5567–81 [2019-12-09]. PMID 25011053. doi:10.18632/oncotarget.1901.

[6] [6]
Liu, YH; Lin, CY; Lin, WC; Tang, SW; Lai, MK; Lin, JY. Up-regulation of vascular endothelial growth factor-D expression in clear cell renal cell carcinoma by CD74: a critical role in cancer cell tumorigenesis.. Journal of immunology (Baltimore, Md. : 1950). 2008-11-01, 181 (9): 6584–94 [2019-12-09]. PMID 18941249. doi:10.4049/jimmunol.181.9.6584.

[7] [7]
Miyake, M; Goodison, S; Lawton, A; Zhang, G; Gomes-Giacoia, E; Rosser, CJ. Erythropoietin is a JAK2 and ERK1/2 effector that can promote renal tumor cell proliferation under hypoxic conditions.. Journal of hematology & oncology. 2013-09-03, 6: 65 [2019-12-09]. PMID 24004818. doi:10.1186/1756-8722-6-65.

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CAKI-1

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CAKI-1

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特徴

科研用途

参考资料

外部链接