Laktaza

Laktaza (EC 3.2.1.108, laktaza-florizinska hidrolaza) je enzim sa sistematskim imenom laktoza galaktohidrolaza.^{[1][2][3][4][5][6]} Ovaj enzim katalizuje sledeću hemijsku reakciju

laktoza + H₂O ${\displaystyle \rightleftharpoons }$ D-galaktoza + D-glukoza

Laktaza
Tetramer laktaze (E. coli)
Identifikatori
EC broj	3.2.1.108
CAS broj	2604851
IntEnz	IntEnz view
BRENDA	BRENDA entry
ExPASy	NiceZyme view
KEGG	KEGG entry
MetaCyc	metabolic pathway
PRIAM	profile
PDB	RCSB PDB PDBe PDBj PDBsum
Pretraga PMC articles PubMed articles NCBI Protein search

Enzim iz intestinalne sluznice je izolovan kao kompleks koji takođe katalizuje reakciju enzima EC 3.2.1.62, glikozilkeramidaza.

Reference

1. Lorenz-Meyer, H., Blum, A.L., Haemmerli, H.P. and Semenza, G. (1972). „A second enzyme defect in acquired lactase deficiency: lack of small-intestinal phlorizin-hydrolase”. Eur. J. Clin. Invest. 2: 326-331. PMID 5082068.
2. Ramaswamay, S. and Radhakrishnan, A.N. (1975). „Lactase-phlorizin hydrolase complex from monkey small intestine. Purification, properties and evidence for two catalytic sites”. Biochim. Biophys. Acta 403: 446-455. PMID 810166.
3. Schlegel-Haueter, S., Hore, P., Kerry, K.R. and Semenza, G. (1972). „The preparation of lactase and glucoamylase of rat small intestine”. Biochim. Biophys. Acta 258: 506-519. PMID 5010299.
4. Skovbjerg, H., Norén, O., Sjöström, H., Danielsen, E.M. and Enevoldsen, B.S. (1982). „Further characterization of intestinal lactase/phlorizin hydrolase”. Biochim. Biophys. Acta 707: 89-97. PMID 6814489.
5. Skovbjerg, H., Sjöström, H. and Norén, O. (1981). „Purification and characterization of amphiphilic lactase-phlorizin hydrolase from human small-intestine”. Eur. J. Biochem. 114: 653-661. PMID 6786877.
6. Asp, N.G., Dahlqvist, A. and Koldovský, O. (1969). „Human small-intestinal β-galactosidases. Separation and characterization of one lactase and one hetero β-galactosidase”. Biochem. J. 114: 351-359. PMID 5822067.

Literatura

• Nicholas C. Price, Lewis Stevens (1999). Fundamentals of Enzymology: The Cell and Molecular Biology of Catalytic Proteins (Third izd.). USA: Oxford University Press. ISBN 019850229X.
• Eric J. Toone (2006). Advances in Enzymology and Related Areas of Molecular Biology, Protein Evolution (Volume 75 izd.). Wiley-Interscience. ISBN 0471205036.
• Branden C, Tooze J.. Introduction to Protein Structure. New York, NY: Garland Publishing. ISBN: 0-8153-2305-0.
• Irwin H. Segel. Enzyme Kinetics: Behavior and Analysis of Rapid Equilibrium and Steady-State Enzyme Systems (Book 44 izd.). Wiley Classics Library. ISBN 0471303097.
• Robert A. Copeland (2013). Evaluation of Enzyme Inhibitors in Drug Discovery: A Guide for Medicinal Chemists and Pharmacologists (2nd izd.). Wiley-Interscience. ISBN 111848813X.
• Gerhard Michal, Dietmar Schomburg (2012). Biochemical Pathways: An Atlas of Biochemistry and Molecular Biology (2nd izd.). Wiley. ISBN 0470146842.

Spoljašnje veze

• MeSH Lactase