E2F2

Transcription factor E2F2 is a protein that in humans is encoded by the E2F2 gene.^[5][6]

E2F2
Available structures PDB Ortholog search: PDBe RCSB List of PDB id codes 1N4M
Identifiers
Aliases	E2F2, E2F-2, E2F transcription factor 2
External IDs	OMIM: 600426; MGI: 1096341; HomoloGene: 48264; GeneCards: E2F2; OMA:E2F2 - orthologs
Gene location (Human) Chr. Chromosome 1 (human)[1] Band 1p36.12 Start 23,506,438 bp[1] End 23,531,233 bp[1]
Gene location (Mouse) Chr. Chromosome 4 (mouse)[2] Band 4|4 D3 Start 135,899,705 bp[2] End 135,923,368 bp[2]
RNA expression pattern Bgee Human Mouse (ortholog) Top expressed in bone marrow bone marrow cells testicle blood gonad mucosa of transverse colon ganglionic eminence ventricular zone monocyte granulocyte Top expressed in blood granulocyte thymus otic vesicle human fetus zygote spleen primary oocyte internal carotid artery hair follicle More reference expression data BioGPS n/a
Gene ontology Molecular function DNA-binding transcription factor activity DNA binding transcription factor binding protein binding protein dimerization activity RNA polymerase II transcription regulatory region sequence-specific DNA binding DNA-binding transcription activator activity, RNA polymerase II-specific sequence-specific DNA binding DNA-binding transcription factor activity, RNA polymerase II-specific cis-regulatory region sequence-specific DNA binding DNA-binding transcription repressor activity, RNA polymerase II-specific Cellular component nucleus transcription regulator complex nucleoplasm RNA polymerase II transcription regulator complex Biological process regulation of cell cycle cell cycle transcription initiation from RNA polymerase II promoter regulation of transcription, DNA-templated lens fiber cell apoptotic process intrinsic apoptotic signaling pathway by p53 class mediator transcription, DNA-templated positive regulation of transcription by RNA polymerase II negative regulation of sprouting angiogenesis negative regulation of transcription by RNA polymerase II Sources:Amigo / QuickGO
Orthologs Species Human Mouse Entrez 1870 242705 Ensembl ENSG00000007968 ENSG00000282899 ENSMUSG00000018983 UniProt Q14209 P56931 RefSeq (mRNA) NM_004091 NM_177733 NM_183301 NM_001305399 RefSeq (protein) NP_004082 NP_001292328 NP_808401 Location (UCSC) Chr 1: 23.51 – 23.53 Mb Chr 4: 135.9 – 135.92 Mb PubMed search [3] [4]
Wikidata
View/Edit Human View/Edit Mouse

Function

The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F1 and E2F3, have an additional cyclin binding domain. This protein binds specifically to retinoblastoma protein pRB in a cell-cycle dependent manner, and it exhibits overall 46% amino acid identity to E2F1.^[7]

Interactions

E2F2 has been shown to interact with:

• BRD2,^[8][9]
• RYBP,^[10] and
• RB1.^[11]

See also

• E2F

References

1. ENSG00000282899 GRCh38: Ensembl release 89: ENSG00000007968, ENSG00000282899 – Ensembl, May 2017
2. GRCm38: Ensembl release 89: ENSMUSG00000018983 – Ensembl, May 2017
3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
5. Ivey-Hoyle M, Conroy R, Huber HE, Goodhart PJ, Oliff A, Heimbrook DC (January 1994). "Cloning and characterization of E2F-2, a novel protein with the biochemical properties of transcription factor E2F". Mol Cell Biol. 13 (12): 7802–12. doi:10.1128/MCB.13.12.7802. PMC 364852. PMID 8246995.
6. Lees JA, Saito M, Vidal M, Valentine M, Look T, Harlow E, Dyson N, Helin K (January 1994). "The retinoblastoma protein binds to a family of E2F transcription factors". Mol Cell Biol. 13 (12): 7813–25. doi:10.1128/mcb.13.12.7813. PMC 364853. PMID 8246996.
7. "Entrez Gene: E2F2 E2F transcription factor 2".
8. Crowley TE, Kaine EM, Yoshida M, Nandi A, Wolgemuth DJ (August 2002). "Reproductive cycle regulation of nuclear import, euchromatic localization, and association with components of Pol II mediator of a mammalian double-bromodomain protein". Mol. Endocrinol. 16 (8): 1727–37. doi:10.1210/me.2001-0353. PMID 12145330. S2CID 14975453.
9. Denis GV, Vaziri C, Guo N, Faller DV (August 2000). "RING3 kinase transactivates promoters of cell cycle regulatory genes through E2F". Cell Growth Differ. 11 (8): 417–24. PMC 3968681. PMID 10965846.
10. Schlisio S, Halperin T, Vidal M, Nevins JR (November 2002). "Interaction of YY1 with E2Fs, mediated by RYBP, provides a mechanism for specificity of E2F function". EMBO J. 21 (21): 5775–86. doi:10.1093/emboj/cdf577. PMC 131074. PMID 12411495.
11. Lee C, Chang JH, Lee HS, Cho Y (December 2002). "Structural basis for the recognition of the E2F transactivation domain by the retinoblastoma tumor suppressor". Genes Dev. 16 (24): 3199–212. doi:10.1101/gad.1046102. PMC 187509. PMID 12502741.

Further reading

• Dyson N (1998). "The regulation of E2F by pRB-family proteins". Genes Dev. 12 (15): 2245–62. doi:10.1101/gad.12.15.2245. PMID 9694791.
• Wu CL, Zukerberg LR, Ngwu C, Harlow E, Lees JA (1995). "In vivo association of E2F and DP family proteins". Mol. Cell. Biol. 15 (5): 2536–46. doi:10.1128/mcb.15.5.2536. PMC 230484. PMID 7739537.
• Karlseder J, Rotheneder H, Wintersberger E (1996). "Interaction of Sp1 with the growth- and cell cycle-regulated transcription factor E2F". Mol. Cell. Biol. 16 (4): 1659–67. doi:10.1128/mcb.16.4.1659. PMC 231152. PMID 8657141.
• Lin SY, Black AR, Kostic D, Pajovic S, Hoover CN, Azizkhan JC (1996). "Cell cycle-regulated association of E2F1 and Sp1 is related to their functional interaction". Mol. Cell. Biol. 16 (4): 1668–75. doi:10.1128/MCB.16.4.1668. PMC 231153. PMID 8657142.
• Rogers KT, Higgins PD, Milla MM, Phillips RS, Horowitz JM (1996). "DP-2, a heterodimeric partner of E2F: identification and characterization of DP-2 proteins expressed in vivo". Proc. Natl. Acad. Sci. U.S.A. 93 (15): 7594–9. Bibcode:1996PNAS...93.7594R. doi:10.1073/pnas.93.15.7594. PMC 38791. PMID 8755520.
• Magae J, Wu CL, Illenye S, Harlow E, Heintz NH (1997). "Nuclear localization of DP and E2F transcription factors by heterodimeric partners and retinoblastoma protein family members". J. Cell Sci. 109 (7): 1717–26. doi:10.1242/jcs.109.7.1717. PMID 8832394.
• Hofmann F, Livingston DM (1996). "Differential effects of cdk2 and cdk3 on the control of pRb and E2F function during G1 exit". Genes Dev. 10 (7): 851–61. doi:10.1101/gad.10.7.851. PMID 8846921.
• Lindeman GJ, Gaubatz S, Livingston DM, Ginsberg D (1997). "The subcellular localization of E2F-4 is cell-cycle dependent". Proc. Natl. Acad. Sci. U.S.A. 94 (10): 5095–100. Bibcode:1997PNAS...94.5095L. doi:10.1073/pnas.94.10.5095. PMC 24637. PMID 9144196.
• Pierce AM, Schneider-Broussard R, Philhower JL, Johnson DG (1998). "Differential activities of E2F family members: unique functions in regulating transcription". Mol. Carcinog. 22 (3): 190–8. doi:10.1002/(SICI)1098-2744(199807)22:3<190::AID-MC7>3.0.CO;2-P. PMID 9688145. S2CID 42749241.
• Halaban R, Cheng E, Smicun Y, Germino J (2000). "Deregulated E2F transcriptional activity in autonomously growing melanoma cells". J. Exp. Med. 191 (6): 1005–16. doi:10.1084/jem.191.6.1005. PMC 2193116. PMID 10727462.
• Takahashi Y, Rayman JB, Dynlacht BD (2000). "Analysis of promoter binding by the E2F and pRB families in vivo: distinct E2F proteins mediate activation and repression". Genes Dev. 14 (7): 804–16. doi:10.1101/gad.14.7.804. PMC 316494. PMID 10766737.
• Denis GV, Vaziri C, Guo N, Faller DV (2001). "RING3 kinase transactivates promoters of cell cycle regulatory genes through E2F". Cell Growth Differ. 11 (8): 417–24. PMC 3968681. PMID 10965846.
• Wu L, Timmers C, Maiti B, Saavedra HI, Sang L, Chong GT, Nuckolls F, Giangrande P, Wright FA, Field SJ, Greenberg ME, Orkin S, Nevins JR, Robinson ML, Leone G (2001). "The E2F1-3 transcription factors are essential for cellular proliferation". Nature. 414 (6862): 457–62. Bibcode:2001Natur.414..457W. doi:10.1038/35106593. PMID 11719808. S2CID 3062825.
• Yamochi T, Semba K, Tsuji K, Mizumoto K, Sato H, Matsuura Y, Nishimoto I, Matsuoka M (2002). "ik3-1/Cables is a substrate for cyclin-dependent kinase 3 (cdk 3)". Eur. J. Biochem. 268 (23): 6076–82. doi:10.1046/j.0014-2956.2001.02555.x. PMID 11733001.
• Weinmann AS, Yan PS, Oberley MJ, Huang TH, Farnham PJ (2002). "Isolating human transcription factor targets by coupling chromatin immunoprecipitation and CpG island microarray analysis". Genes Dev. 16 (2): 235–44. doi:10.1101/gad.943102. PMC 155318. PMID 11799066.
• Crowley TE, Kaine EM, Yoshida M, Nandi A, Wolgemuth DJ (2003). "Reproductive cycle regulation of nuclear import, euchromatic localization, and association with components of Pol II mediator of a mammalian double-bromodomain protein". Mol. Endocrinol. 16 (8): 1727–37. doi:10.1210/me.2001-0353. PMID 12145330. S2CID 14975453.
• Schlisio S, Halperin T, Vidal M, Nevins JR (2002). "Interaction of YY1 with E2Fs, mediated by RYBP, provides a mechanism for specificity of E2F function". EMBO J. 21 (21): 5775–86. doi:10.1093/emboj/cdf577. PMC 131074. PMID 12411495.

External links

• E2F2+protein,+human at the U.S. National Library of Medicine Medical Subject Headings (MeSH)

This article incorporates text from the United States National Library of Medicine, which is in the public domain.