二氫硫辛醯胺脫氫酶（EC 1.8.1.4，英語：Dihydrolipoamide dehydrogenase，縮寫DLD，又稱為粒線體二氫硫辛酸脫氫酶，dihydrolipoyl dehydrogenase, mitochondrial）是一種由人類基因DLD[1][2][3][4]所編碼的黃素蛋白，其作用是將二氫硫辛醯胺脫氫從而轉化為氧化型硫辛醯胺。
Quick Facts 二氫硫辛醯胺脫氫酶, 有效結構 ...
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Quick Facts 二氫硫辛醯胺脫氫酶, 命名 ...
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DLD作為一種粒線體蛋白質，在真核生物的能量代謝中起到重要作用，它至少參與了五種多酶複合體，且為複合體完成反應所必需的組份[5]。另外，DLD作為一種黃素蛋白氧化還原酶，以FAD為輔基接受質子與電子催化二硫鍵的形成。
DLD是大小為51千道爾頓亞基的同二聚體，其中每個亞基都與一分子的FAD以共價鍵的形式相連[6]。
在哺乳動物的粒線體三羧酸循環中，該酶與丙酮酸脫氫酶和二氫硫辛醯基乙醯基轉基酶一起組成中丙酮酸脫氫酶複合體，同時該酶亦是α-酮戊二酸脫氫酶複合體中的一個組分。二氫硫辛醯胺脫氫酶的主要任務是負責將複合物中另一組分硫辛醯胺轉乙醯基酶（或二氫硫辛醯琥珀醯轉移酶）的輔基二氫硫辛醯胺脫氫從而轉化為氧化型硫辛醯胺，該酶也是粒線體甘氨酸剪切體系的組分。
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