A2058

A2058 （亦称ATCC CRL-11147）是人类恶性黑色素瘤细胞系，最初分离自一位43岁的人类男性转移性脑癌（英语：Brain metastasis）患者^[1]。A2058细胞具有高度移动特性，并且表达神经生长因子（英语：Nerve growth factor）^[2]及神经生长因子受体^[3]。高度糖基化的CD93跨膜蛋白分布在A2058细胞的细胞膜，尤其在细胞与细胞接触的位置，其延展面积比载体控制细胞为大，并且促进细胞间紧密的接触，故而加入CD93抗体及耗尽细胞培养基中的钙离子会破坏以CD93蛋白作为媒介的A2058细胞贴附^[4]。有研究指出蜜蜂蜂毒会诱发A2058细胞的凋亡，短时间内便可令胞体外与内质网的钙离子进入细胞内，所以钙离子在细胞内的浓度会快速上升^[5]。

参考资料

1. Todaro, GJ; Fryling, C; De Larco, JE. Transforming growth factors produced by certain human tumor cells: polypeptides that interact with epidermal growth factor receptors.. Proceedings of the National Academy of Sciences of the United States of America. 1980-09, 77 (9): 5258–62 [2019-12-06]. PMID 6254071. doi:10.1073/pnas.77.9.5258.
2. Fabricant, RN; De Larco, JE; Todaro, GJ. Nerve growth factor receptors on human melanoma cells in culture.. Proceedings of the National Academy of Sciences of the United States of America. 1977-02, 74 (2): 565–9 [2019-12-06]. PMID 265522. doi:10.1073/pnas.74.2.565.
3. Sherwin, SA; Sliski, AH; Todaro, GJ. Human melanoma cells have both nerve growth factor and nerve growth factor-specific receptors on their cell surfaces.. Proceedings of the National Academy of Sciences of the United States of America. 1979-03, 76 (3): 1288–92 [2019-12-06]. PMID 375235. doi:10.1073/pnas.76.3.1288.
4. CHEN Hu-yan, WANG Shang-shang, JIANG Min, ZHANG Qiao-an, CHEN Lian-jun. Inhibitory effect of microRNA-205 on migration and invasion of melanoma cell lines and its related mechanism. Academic Journal of Second Military Medical University, 2017, 38(5): 576-82 [2019-12-06]
5. Tu, WC; Wu, CC; Hsieh, HL; Chen, CY; Hsu, SL. Honeybee venom induces calcium-dependent but caspase-independent apoptotic cell death in human melanoma A2058 cells.. Toxicon : official journal of the International Society on Toxinology. 2008-08-01, 52 (2): 318–29 [2019-12-06]. PMID 18602939. doi:10.1016/j.toxicon.2008.06.007.

外部链接

• Cellosaurus entry for A2058（页面存档备份，存于互联网档案馆）