T4噬菌体

T4噬菌体是噬菌体的一个品系，属于T－系噬菌体，为烈性噬菌体。具有典型的蝌蚪状外形：六角形的头部和可收缩的长的尾部。头部的蛋白质外壳内含有折叠的DNA分子；尾部的蛋白质外壳为一中空的长管，外面包有可收缩的尾鞘。头部大小为65*95nm，颈部长95nm，尾部120nm。

Bacteriophage T4 Structural Model at Atomic Resolution^[1]

T4噬菌体
病毒分类
（未分级）：	病毒 Virus
域：	双链DNA病毒域 Duplodnaviria
界：	香港病毒界 Heunggongvirae
门：	尾噬菌体门 Uroviricota
纲：	有尾噬菌体纲 Caudoviricetes
目：	有尾噬菌体目 Caudovirales
科：	肌尾噬菌体科 Myoviridae
属：	T4噬菌体属 T4virus
种：	T4噬菌体 Enterobacteria phage T4

介绍

侵染寄主时，尾鞘收缩，头部的DNA即通过中空的尾部注入细胞内。T－系噬菌体是研究最广泛和深入的细菌噬菌体，侵染大肠杆菌（一种正常栖居在人类肠道中的细菌）B菌株，T为英文Type的字头，并按其发现的前后顺序编号为T1，2，3，4，5，6，7，因其偶数型（2，4，6）结构的化学组成与奇数型（1，3，5，7）不同，故又分为T－偶数系与T－奇数系。例：T4属偶数系。

结构

其构造属复合对称体制。因结构简单、遗传背景清楚、增殖容易、对人无害，故是迄今被研究得最透彻、在病毒学和分子遗传学研究中应用最广泛的模式生物之一。构造分头部、颈部和尾部3部分。头部呈拉长的二十面体结构，长95nm，宽65 nm，衣壳由9～19种蛋白质组成，蛋白质含量占 76%～81%，含有直径为6nm的衣壳粒212个。核心由线状dsDNA组成，长约50μm(约为头长的650倍)，由1.7×10bp构成(包括约2.3%末端冗余)。 DNA成分特殊，以5-羟甲基胞嘧啶(HMC)取代常见的胞嘧啶。头、尾部相连处有一颈部，包括颈环和颈须，前者为一六角形盘状结构，直径37.5nm，每一角上各长一颈须，用以裹住吸附前的尾丝。长而可收缩的尾部由尾鞘、尾管、基板、刺突和尾丝5部分组成。尾鞘长95、宽22 nm，是一种由相对分子质量为55 000的衣壳粒共144个缠绕而成的24环螺旋组成。尾管长95 nm，直径8nm，中央孔道的直径为2.5～3.5 nm，它是头部核酸即基因组注入寄主细胞时的必经之路。尾管亦由24环螺旋组成，与尾鞘相对应。基板与颈环相似，为一有中央尾孔的六角形盘状物，直径为30.5 nm，上长6个刺突和6根尾丝。刺突长20nm，有吸附功能。尾丝可折成2段，长140nm，直径3～ 4nm，由2种相对分子质量较大的蛋白质和4种相对分子质量较小的蛋白质组成，能专一地吸附在敏感寄主细胞表面的相应受体上。T4的尾孔至少含溶菌酶等8种蛋白质。3种T偶数噬菌体在寄主细胞表面有不同的吸附位点：T4在大肠埃希氏菌(Escherichia coli)B菌株上的吸附位点是LPS核心多糖中的α-葡糖基-(1→3)葡萄糖末端，在K菌株上则为LPS或外膜蛋白 OmpC。 科学家们在T4噬菌体中发现了一种酶，称为T4-DNA连接酶，在转基因技术中用作黏性末端和平末端的连接酶。对平末端连接效率低下。

参考

1. Padilla-Sanchez, Victor. Structural Model of Bacteriophage T4. WikiJournal of Science. 2021, 4 (1): 5 [2021-08-06]. doi:10.15347/WJS/2021.005. （原始内容存档于2022-04-28）.

延伸阅读

• Padilla-Sanchez, Victor. Structural Model of Bacteriophage T4. WikiJournal of Science. 2021, 4 (1): 5 [2021-08-06]. doi:10.15347/WJS/2021.005. （原始内容存档于2022-04-28）.
• Leiman P.G.; Kanamaru S; Mesyanzhinov V.V.; Arisaka F.; Rossmann M.G. Structure and morphogenesis of bacteriophage T4.. Cellulal and Molecular Life Sciences CMLS. 2003, 60 (11): 2356–2370. doi:10.1007/s00018-003-3072-1.
• Karam, J., Petrov, V., Nolan, J., Chin, D., Shatley, C., Krisch, H., and Letarov, A. The T4-like phages genome project. https://web.archive.org/web/20070523215704/http://phage.bioc.tulane.edu/. (The T4-like phage full genomic sequence depository)
• Mosig, G., and F. Eiserling. 2006. T4 and related phages: structure and development, R. Calendar and S. T. Abedon (eds.), The Bacteriophages. Oxford University Press, Oxford. (Review of phage T4 biology) ISBN 0-19-514850-9
• Filee J. Tetart F.; Suttle C.A.; Krisch H.M. Marine T4-type bacteriophages, a ubiquitous component of the dark matter of the biosphere. Proc. Natl. Acad. Sci. USA. 2005, 102 (35): 12471–6 [2018-04-08]. PMC 1194919 . PMID 16116082. doi:10.1073/pnas.0503404102. （原始内容存档于2008-06-20）. (Indication of prevalence and T4-like phages in the wild)
• Chibani-Chennoufi S.; Canchaya C.; Bruttin A.; Brussow H. Comparative genomics of the T4-Like Escherichia coli phage JS98: implications for the evolution of T4 phages. J. Bacteriol. 2004, 186 (24): 8276–86 [2018-04-08]. PMC 532421 . PMID 15576776. doi:10.1128/JB.186.24.8276-8286.2004. （原始内容存档于2011-05-11）. (Characterization of a T4-like phage)
• Desplats C, Krisch HM. The diversity and evolution of the T4-type bacteriophages. Res. Microbiol. May 2003, 154 (4): 259–67 [2018-04-08]. PMID 12798230. doi:10.1016/S0923-2508(03)00069-X. （原始内容存档于2019-02-18）.
• Miller, E.S.; Kutter E.; Mosig G.; Arisaka F.; Kunisawa T.; Ruger W. Bacteriophage T4 genome. Microbiol. Mol. Biol. Rev. 2003, 67 (1): 86–156 [2018-04-08]. PMC 150520 . PMID 12626685. doi:10.1128/MMBR.67.1.86-156.2003. （原始内容存档于2011-05-11）. (Review of phage T4, from the perspective of its genome)
• Desplats C.; Dez C.; Tetart F.; Eleaume H.; Krisch H.M. Snapshot of the genome of the pseudo-T-even bacteriophage RB49. J. Bacteriol. 2002, 184 (10): 2789–2804 [2018-04-08]. PMC 135041 . PMID 11976309. doi:10.1128/JB.184.10.2789-2804.2002. （原始内容存档于2011-05-11）. (Overview of the RB49 genome, a T4-like phage)
• Malys N, Chang DY, Baumann RG, Xie D, Black LW. A bipartite bacteriophage T4 SOC and HOC randomized peptide display library: detection and analysis of phage T4 terminase (gp17) and late sigma factor (gp55) interaction. J Mol Biol. 2002, 319 (2): 289–304. PMID 12051907. doi:10.1016/S0022-2836(02)00298-X. (T4 phage application in biotechnology for studying protein interaction)
• Tétart F.; Desplats C.; Kutateladze M.; Monod C.; Ackermann H.-W.; Krisch H.M. Phylogeny of the major head and tail genes of the wide-ranging T4-type bacteriophages. J. Bacteriol. 2001, 183 (1): 358–366 [2018-04-08]. PMC 94885 . PMID 11114936. doi:10.1128/JB.183.1.358-366.2001. （原始内容存档于2011-05-11）. (Indication of the prevalence of T4-type sequences in the wild)
• Abedon S.T. The murky origin of Snow White and her T-even dwarfs. Genetics. 2000, 155 (2): 481–6 [2018-04-08]. PMC 1461100 . PMID 10835374. （原始内容存档于2011-01-28）. (Historical description of the isolation of the T4-like phages T2, T4, and T6)
• Ackermann HW, Krisch HM. A catalogue of T4-type bacteriophages. Arch. Virol. 1997, 142 (12): 2329–45 [2018-04-08]. PMID 9672598. doi:10.1007/s007050050246. （原始内容存档于2001-11-01）. (Nearly complete list of then-known T4-like phages)
• Monod C, Repoila F, Kutateladze M, Tétart F, Krisch HM. The genome of the pseudo T-even bacteriophages, a diverse group that resembles T4. J. Mol. Biol. March 1997, 267 (2): 237–49 [2018-04-08]. PMID 9096222. doi:10.1006/jmbi.1996.0867. （原始内容存档于2019-02-18）. (Overview of various T4-like phages from the perspective of their genomes)
• Kutter E.; Gachechiladze K.; Poglazov A.; Marusich E.; Shneider M.; Aronsson P.; Napuli A.; Porter D.; Mesyanzhinov V. Evolution of T4-related phages. Virus Genes. 1995, 11 (2–3): 285–297. PMID 8828153. doi:10.1007/BF01728666.^{[永久失效链接]} (Comparison of the genomes of various T4-like phages)
• Karam, J. D. et al. 1994. Molecular Biology of Bacteriophage T4. ASM Press, Washington, DC. (The second T4 bible, go here, as well as Mosig and Eiserling, 2006, to begin to learn about the biology T4 phage) ISBN 1-55581-064-0
• Eddy, S. R. 1992. Introns in the T-Even Bacteriophages. Ph.D. thesis. University of Colorado at Boulder. (Chapter 3 provides overview of various T4-like phages as well as the isolation of then-new T4-like phages)
• Surdis, T.J "et al" Bacteriophage attachment methods specific to T4, analysis, Overview.
• Mathews, C. K., E. M. Kutter, G. Mosig, and P. B. Berget. 1983. Bacteriophage T4. American Society for Microbiology, Washington, DC. (The first T4 bible; not all information here is duplicated in Karam et al., 1994; see especially the introductory chapter by Doermann for a historical overview of the T4-like phages) ISBN 0-914826-56-5
• Russell, R. L. 1967. Speciation Among the T-Even Bacteriophages. Ph.D. thesis. California Institute of Technology. (Isolation of the RB series of T4-like phages)
• Malys N, Nivinskas R. Non-canonical RNA arrangement in T4-even phages: accommodated ribosome binding site at the gene 26-25 intercistronic junction. Mol Microbiol. 2009, 73 (6): 1115–1127. PMID 19708923. doi:10.1111/j.1365-2958.2009.06840.x. (rare type of translational regulation characterized in T4)
• Kay D.; Fildes P. Hydroxymethylcytosine-containing and tryptophan-dependent bacteriophages isolated from city effluents. J. Gen. Microbiol. 1962, 27: 143–6. PMID 14454648. doi:10.1099/00221287-27-1-143. (T4-like phage isolation, including that of phage Ox2)

参阅

• 病毒主题

• 噬菌体

外部链接

• Viralzone: T4-like viruses（页面存档备份，存于互联网档案馆）
• T4 Bacteriophage Infection Process Animations（页面存档备份，存于互联网档案馆）

警告：默认排序键“Enterobacteria Phage T4”覆盖了之前的默认排序键“Enterobacteria phage T4”。