Neuro-2a

Neuro-2a是快速生长的小鼠神经母细胞瘤细胞系^[1]。Neuro-2a细胞目前已被用于研究神经突的生长^[1]、神经毒性^[2]、阿兹海默症^[3]、哺乳动物细胞系的非对称性细胞分裂（英语：Asymmetric cell division）^[4]、腺病毒转导及狂犬病的诊断。

3 sources

特征

Neuro-2a细胞起源于小鼠，具有神经元和变形虫干细胞的形态，可以根据环境因素进行细胞分化，而已分化的细胞具有神经元的许多特性，例如神经纤维丝（英语：Neurofilament）。这些细胞可能因经过多次传代而对毒素的反应不同于活生物体内的神经元细胞^[2]。Neuro-2a细胞合成大量的微管，因此容易受到单纯疱疹病毒和脊髓灰质炎病毒等的感染，这些病毒会改变细胞的形态和生理学。

1 sources

参考资料

[1]
Salto, R; Vílchez, JD; Girón, MD; Cabrera, E; Campos, N; Manzano, M; Rueda, R; López-Pedrosa, JM. β-Hydroxy-β-Methylbutyrate (HMB) Promotes Neurite Outgrowth in Neuro2a Cells.. PloS one. 2015, 10 (8): e0135614 [2020-01-06]. PMID 26267903. doi:10.1371/journal.pone.0135614.^{[永久失效链接]}
[2]
LePage, KT; Dickey, RW; Gerwick, WH; Jester, EL; Murray, TF. On the use of neuro-2a neuroblastoma cells versus intact neurons in primary culture for neurotoxicity studies.. Critical reviews in neurobiology. 2005, 17 (1): 27–50 [2020-01-06]. PMID 16307526. doi:10.1615/critrevneurobiol.v17.i1.20.^{[永久失效链接]}
[3]
Provost, P. Interpretation and applicability of microRNA data to the context of Alzheimer's and age-related diseases.. Aging. 2010-03-31, 2 (3): 166–9 [2020-01-06]. PMID 20375468. doi:10.18632/aging.100131.^{[永久失效链接]}
[4]
Ogrodnik, M; Salmonowicz, H; Brown, R; Turkowska, J; Średniawa, W; Pattabiraman, S; Amen, T; Abraham, AC; Eichler, N; Lyakhovetsky, R; Kaganovich, D. Dynamic JUNQ inclusion bodies are asymmetrically inherited in mammalian cell lines through the asymmetric partitioning of vimentin.. Proceedings of the National Academy of Sciences of the United States of America. 2014-06-03, 111 (22): 8049–54 [2020-01-06]. PMID 24843142. doi:10.1073/pnas.1324035111.^{[永久失效链接]}

外部链接

N2a at ATCC (CCL-131)（页面存档备份，存于互联网档案馆）
N2a at ECACC (89121404)（页面存档备份，存于互联网档案馆）
Cellosaurus entry for N2a（页面存档备份，存于互联网档案馆）

[a-1] [1]
Salto, R; Vílchez, JD; Girón, MD; Cabrera, E; Campos, N; Manzano, M; Rueda, R; López-Pedrosa, JM. β-Hydroxy-β-Methylbutyrate (HMB) Promotes Neurite Outgrowth in Neuro2a Cells.. PloS one. 2015, 10 (8): e0135614 [2020-01-06]. PMID 26267903. doi:10.1371/journal.pone.0135614.^{[永久失效链接]}

[b-2] [2]
LePage, KT; Dickey, RW; Gerwick, WH; Jester, EL; Murray, TF. On the use of neuro-2a neuroblastoma cells versus intact neurons in primary culture for neurotoxicity studies.. Critical reviews in neurobiology. 2005, 17 (1): 27–50 [2020-01-06]. PMID 16307526. doi:10.1615/critrevneurobiol.v17.i1.20.^{[永久失效链接]}

[3] [3]
Provost, P. Interpretation and applicability of microRNA data to the context of Alzheimer's and age-related diseases.. Aging. 2010-03-31, 2 (3): 166–9 [2020-01-06]. PMID 20375468. doi:10.18632/aging.100131.^{[永久失效链接]}

[4] [4]
Ogrodnik, M; Salmonowicz, H; Brown, R; Turkowska, J; Średniawa, W; Pattabiraman, S; Amen, T; Abraham, AC; Eichler, N; Lyakhovetsky, R; Kaganovich, D. Dynamic JUNQ inclusion bodies are asymmetrically inherited in mammalian cell lines through the asymmetric partitioning of vimentin.. Proceedings of the National Academy of Sciences of the United States of America. 2014-06-03, 111 (22): 8049–54 [2020-01-06]. PMID 24843142. doi:10.1073/pnas.1324035111.^{[永久失效链接]}

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