豬籠草蛋白酶

豬籠草蛋白酶（Nepenthesin，Nepenthacin^[1][2]，Nepenthasin^[3]），又稱豬籠草酸性蛋白酶，豬籠草天冬氨酸蛋白酶^[3]是豬籠草捕蟲籠及盾葉茅膏菜（Drosera peltata）分泌的天冬氨酸蛋白酶。^{[4][5][6][7][8][9]}其與胃蛋白酶類似，但其也能酶切兩側的天冬氨酸殘基及賴氨酸┼精氨酸。^[3]其對pH值及溫度的穩定性高於豬胃蛋白酶A，對尿素及鹽酸胍的穩定性大大降低。^[10]

豬籠草蛋白酶
命名 系統命名 縮寫
識別碼
EC編號	3.4.23.12
CAS號	9073-80-7
數據庫
IntEnz	IntEnz瀏覽
BRENDA（英語：BRENDA）	BRENDA入口
ExPASy（英語：ExPASy）	NiceZyme瀏覽
KEGG	KEGG入口
MetaCyc（英語：MetaCyc）	代謝路徑
PRIAM（英語：PRIAM_enzyme-specific_profiles）	概述
PDB	RCSB PDB PDBj PDBe PDBsum
搜索 PMC 相關文獻 PubMed 相關文獻

1968年，Shigeru Nakayama和Shizuko Amagase以「nepenthesin」命名豬籠草蛋白酶。^[11]在豬籠草屬中已確定了2個同工酶：豬籠草蛋白酶I與豬籠草蛋白酶II。^[12]

參考文獻

1. Jentsch J. Enzymes from carnivorous plants (nepenthes). Isolation of the protease nepenthacin. FEBS Lett. April 1972, 21 (3): 273–276. PMID 11946525. doi:10.1016/0014-5793(72)80181-9.
2. Jentsch J, Meierkord S, Hammer M. The enzymes from carnivorous plants (Nepenthes): Properties and characterization of the acid protease nepenthacin. Planta Medica. 1989, 55: 227. doi:10.1055/s-2006-961979.
3. EC 3.4.23.12 - Nepenthesin （頁面存檔備份，存於互聯網檔案館）. Integrated Enzyme Database (IntEnz).
4. Amagase S, Nakayama S, Tsugita A. Acid protease in Nepenthes. II. Study on the specificity of nepenthesin. J. Biochem. October 1969, 66 (4): 431–9. PMID 5354017.
5. Amagase S. Digestive enzymes in insectivorous plants. 3. Acid proteases in the genus Nepenthes and Drosera peltata. J. Biochem. July 1972, 72 (1): 73–81. PMID 5069751.
6. Amagase S, Mori M, Nakayama S. Digestive enzymes in insectivorous plants. IV. Enzymatic digestion of insects by Nepenthes secretion and Drosera peltata extract: proteolytic and chitinolytic activities. J. Biochem. September 1972, 72 (3): 765–7. PMID 4634982.
7. Tökés ZA, Woon WC, Chambers SM. Digestive enzymes secreted by the carnivorous plant Nepenthes macferlanei L. Planta. March 1974, 119 (1): 39–46. doi:10.1007/BF00390820.
8. Athauda SBP, Inoue H, Iwamatsu A, Takahashi K. Acid Proteinase from Nepenthes distillatoria (Badura). James, Michael (編). Aspartic proteinases: retroviral and cellular enzymes. New York: Plenum. 1998: 453–458. ISBN 0-306-45809-8.
9. Takahashi K, Tanji M, Shibata C. Variations in the content and isozymic composition of nepenthesin in the pitcher fluids among Nepenthes species (PDF). Carnivorous Plant News Letter. 2007, 36 (3): 73–76 [2011-12-10]. （原始內容存檔 (PDF)於2012-02-27）.
10. Kubota K, Metoki Y, Athauda SBP, Shibata C, Takahashi K. Stability Profiles of Nepenthesin in Urea and Guanidine Hydrochloride: Comparison with Porcine Pepsin A. Bioscience, Biotechnology, and Biochemistry. 2010, 74 (11): 2323–2326. doi:10.1271/bbb.100391.
11. Nakayama S, Amagase S. Acid Protease in Nepenthes: Partial Purification and Properties of the Enzyme. Proceedings of the Japan Academy. 1968, 44 (5): 358–362.^{[永久失效連結]}
12. Athauda SB, Matsumoto K, Rajapakshe S, Kuribayashi M, Kojima M, Kubomura-Yoshida N, Iwamatsu A, Shibata C, Inoue H, Takahashi K. Enzymic and structural characterization of nepenthesin, a unique member of a novel subfamily of aspartic proteinases. Biochem. J. July 2004, 381 (Pt 1): 295–306. PMC 1133788 . PMID 15035659. doi:10.1042/BJ20031575.

擴展閱讀

• The MEROPS online database for peptidases and their inhibitors: A01.040