EcoRV(V是5的意思)是一種第二型限制內切酶,來自某些特定品系的大腸桿菌(Escherichia coli),此細菌的學名也是EcoRV前三個字母的由來。
EcoRV是分子生物學與生物技術上常使用的限制酶,可辨識出一段含有6個鹼基的5'GAT|ATC-3'(|為切開的位置)DNA序列,以及互補的3'-CTA|TAG-5',以產生平整末端的方式將此序列切開。
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