主要鹼性蛋白

主要鹼性蛋白（英語：Major basic protein，MBP），全稱為嗜曙紅顆粒白血球主要鹼性蛋白（英語：Eosinophil Major basic protein），也稱為蛋白多糖2（Proteoglycan 2，PRG2），是一種由人類基因PRG2編碼的蛋白質^[5]。

主要鹼性蛋白
已知的結構 PDB 直系同源搜尋: PDBe RCSB PDBID列表 1H8U、​2BRS、​4QXX
識別號
別名	PRG2；, BMPG, MBP, MBP1, proteoglycan 2, bone marrow (natural killer cell activator, eosinophil granule major basic protein), proMBP, proteoglycan 2, pro eosinophil major basic protein
外部ID	OMIM：605601 MGI：103294 HomoloGene：2044 GeneCards：PRG2
基因位置（人類） 染色體 11號染色體[1] 基因座 11q12.1 起始 57,386,780 bp[1] 終止 57,390,650 bp[1]
基因位置（小鼠） 染色體 小鼠2號染色體[2] 基因座 2 D|2 49.45 cM 起始 84,810,805 bp[2] 終止 84,813,976 bp[2]
RNA表達模式
查閱更多表達數據
基因本體 分子功能 • heparin binding • carbohydrate binding • extracellular matrix structural constituent conferring compression resistance 細胞組分 • 細胞外區域 • transport vesicle • cytoplasmic vesicle • 外排體 • ficolin-1-rich granule lumen • collagen-containing extracellular matrix 生物學過程 • defense response to bacterium • immune system process • defense response to nematode • negative regulation of interleukin-10 production • positive regulation of interleukin-4 production • 免疫反應 • neutrophil degranulation Sources:Amigo / QuickGO
直系同源
物種	人類	小鼠
Entrez	5553	19074
Ensembl	ENSG00000186652	ENSMUSG00000027073
UniProt	P13727	Q61878
mRNA​序列	NM_002728 ​NM_001243245 ​NM_001302926 ​NM_001302927	NM_008920
蛋白序列	NP_001230174 ​NP_001289855 ​NP_001289856 ​NP_002719	NP_032946
基因位置​（UCSC）	Chr 11: 57.39 – 57.39 Mb	Chr 2: 84.81 – 84.81 Mb
PubMed​查找	[3]	[4]
維基數據
檢視/編輯人類 檢視/編輯小鼠

MBP在結構上類似於凝集素（糖結合蛋白），特別是與C-型凝集素在摺疊上相似。但與C-型凝集素不同（C-型凝集素在鈣的存在下會結合各種碳水化合物），MBP既不與鈣結合也不與這些糖類結合，而是識別並結合硫酸乙酰肝素（heparan sulfate）。MBP的兩種晶體結構已被測定。^[6][7]

相互作用

主要鹼性蛋白已知可與妊娠相關血漿蛋白A發生蛋白質交互作用。^[8][9][10]

參考文獻

1. GRCh38: Ensembl release 89: ENSG00000186652 - Ensembl, May 2017
2. GRCm38: Ensembl release 89: ENSMUSG00000027073 - Ensembl, May 2017
3. Human PubMed Reference:. National Center for Biotechnology Information, U.S. National Library of Medicine.
4. Mouse PubMed Reference:. National Center for Biotechnology Information, U.S. National Library of Medicine.
5. Entrez Gene: PRG2 proteoglycan 2, bone marrow (natural killer cell activator, eosinophil granule major basic protein). （原始內容存檔於2008-11-02）.
6. PDB 1h8u; Swaminathan GJ, Weaver AJ, Loegering DA, Checkel JL, Leonidas DD, Gleich GJ, Acharya KR. Crystal structure of the eosinophil major basic protein at 1.8 A. An atypical lectin with a paradigm shift in specificity. J. Biol. Chem. July 2001, 276 (28): 26197–26203. PMID 11319227. doi:10.1074/jbc.M100848200.
7. PDB 2brs; Swaminathan GJ, Myszka DG, Katsamba PS, Ohnuki LE, Gleich GJ, Acharya KR. Eosinophil-granule major basic protein, a C-type lectin, binds heparin. Biochemistry. November 2005, 44 (43): 14152–14158. PMID 16245931. doi:10.1021/bi051112b.
8. Overgaard, M T; Haaning J; Boldt H B; Olsen I M; Laursen L S; Christiansen M; Gleich G J; Sottrup-Jensen L; Conover C A; Oxvig C. Expression of recombinant human pregnancy-associated plasma protein-A and identification of the proform of eosinophil major basic protein as its physiological inhibitor. J. Biol. Chem. (UNITED STATES). October 2000, 275 (40): 31128–31133. ISSN 0021-9258. PMID 10913121. doi:10.1074/jbc.M001384200.
9. Overgaard MT, Sorensen ES, Stachowiak D, Boldt HB, Kristensen L, Sottrup-Jensen L, Oxvig C. Complex of pregnancy-associated plasma protein-A and the proform of eosinophil major basic protein. Disulfide structure and carbohydrate attachment. J. Biol. Chem. (United States). January 2003, 278 (4): 2106–2117. ISSN 0021-9258. PMID 12421832. doi:10.1074/jbc.M208777200.
10. Oxvig, C; Sand O; Kristensen T; Gleich G J; Sottrup-Jensen L. Circulating human pregnancy-associated plasma protein-A is disulfide-bridged to the proform of eosinophil major basic protein. J. Biol. Chem. (UNITED STATES). June 1993, 268 (17): 12243–6. ISSN 0021-9258. PMID 7685339.

外部連結

• 醫學主題詞表（MeSH）：Eosinophil+Major+Basic+Protein

延伸閱讀

• Vanhaesebroeck B, Alessi DR. The PI3K-PDK1 connection: more than just a road to PKB. Biochem. J. 2000, 346 (3): 561–76. PMC 1220886 . PMID 10698680. doi:10.1042/0264-6021:3460561.
• Yoshimatsu K, Ohya Y, Shikata Y, et al. Purification and cDNA cloning of a novel factor produced by a human T-cell hybridoma: sequence homology with animal lectins. Mol. Immunol. 1992, 29 (4): 537–546. PMID 1565101. doi:10.1016/0161-5890(92)90012-M.
• Barker RL, Loegering DA, Arakawa KC, et al. Cloning and sequence analysis of the human gene encoding eosinophil major basic protein. Gene. 1990, 86 (2): 285–289. PMID 2323577. doi:10.1016/0378-1119(90)90292-Y.
• Gabay JE, Scott RW, Campanelli D, et al. Antibiotic proteins of human polymorphonuclear leukocytes. Proc. Natl. Acad. Sci. U.S.A. 1989, 86 (14): 5610–5614. Bibcode:1989PNAS...86.5610G. PMC 297672 . PMID 2501794. doi:10.1073/pnas.86.14.5610.
• Wasmoen TL, McKean DJ, Benirschke K, et al. Evidence of eosinophil granule major basic protein in human placenta. J. Exp. Med. 1990, 170 (6): 2051–2063. PMC 2189540 . PMID 2584934. doi:10.1084/jem.170.6.2051.
• Barker RL, Gleich GJ, Pease LR. Acidic precursor revealed in human eosinophil granule major basic protein cDNA. J. Exp. Med. 1988, 168 (4): 1493–1498. PMC 2189086 . PMID 3171483. doi:10.1084/jem.168.4.1493.
• McGrogan M, Simonsen C, Scott R, et al. Isolation of a complementary DNA clone encoding a precursor to human eosinophil major basic protein. J. Exp. Med. 1989, 168 (6): 2295–2308. PMC 2189145 . PMID 3199069. doi:10.1084/jem.168.6.2295.
• Wasmoen TL, Bell MP, Loegering DA, et al. Biochemical and amino acid sequence analysis of human eosinophil granule major basic protein. J. Biol. Chem. 1988, 263 (25): 12559–63. PMID 3410852.
• Weller PF, Ackerman SJ, Smith JA. Eosinophil granule cationic proteins: major basic protein is distinct from the smaller subunit of eosinophil peroxidase. J. Leukoc. Biol. 1988, 43 (1): 1–4. PMID 3422083.
• Kristensen T, Oxvig C, Sand O, et al. Amino acid sequence of human pregnancy-associated plasma protein-A derived from cloned cDNA. Biochemistry. 1994, 33 (6): 1592–1598. PMID 7508748. doi:10.1021/bi00172a040.
• Oxvig C, Haaning J, Højrup P, Sottrup-Jensen L. Location and nature of carbohydrate groups in proform of human major basic protein isolated from pregnancy serum. Biochem. Mol. Biol. Int. 1994, 33 (2): 329–36. PMID 7524900.
• Bonno M, Oxvig C, Kephart GM, et al. Localization of pregnancy-associated plasma protein-A and colocalization of pregnancy-associated plasma protein-A messenger ribonucleic acid and eosinophil granule major basic protein messenger ribonucleic acid in placenta. Lab. Invest. 1994, 71 (4): 560–6. PMID 7526035.
• Li MS, Sun L, Satoh T, et al. Human eosinophil major basic protein, a mediator of allergic inflammation, is expressed by alternative splicing from two promoters. Biochem. J. 1995, 305 (3): 921–7. PMC 1136346 . PMID 7531438.
• Oxvig C, Haaning J, Kristensen L, et al. Identification of angiotensinogen and complement C3dg as novel proteins binding the proform of eosinophil major basic protein in human pregnancy serum and plasma. J. Biol. Chem. 1995, 270 (23): 13645–13651. PMID 7539791. doi:10.1074/jbc.270.23.13645.
• Oxvig C, Sand O, Kristensen T, et al. Circulating human pregnancy-associated plasma protein-A is disulfide-bridged to the proform of eosinophil major basic protein. J. Biol. Chem. 1993, 268 (17): 12243–6. PMID 7685339.
• Levi-Schaffer F, Lacy P, Severs NJ, et al. Association of granulocyte-macrophage colony-stimulating factor with the crystalloid granules of human eosinophils. Blood. 1995, 85 (9): 2579–86. PMID 7727786.
• Oxvig C, Gleich GJ, Sottrup-Jensen L. Localization of disulfide bridges and free sulfhydryl groups in human eosinophil granule major basic protein. FEBS Lett. 1994, 341 (2–3): 213–217. PMID 8137941. doi:10.1016/0014-5793(94)80459-1.
• Shikata Y, Hayashi Y, Yoshimatsu K, et al. Pro-major basic protein has three types of sugar chains at the pro-portion. Biochim. Biophys. Acta. 1993, 1163 (3): 243–9. PMID 8507662. doi:10.1016/0167-4838(93)90158-N.
• Nittoh T, Watanabe M, Okayama H, et al. Cloning of cDNA for rat eosinophil major basic protein. Biochim. Biophys. Acta. 1996, 1264 (3): 261–4. PMID 8547309. doi:10.1016/0167-4781(95)00183-2.
• Delcommenne M, Tan C, Gray V, et al. Phosphoinositide-3-OH kinase-dependent regulation of glycogen synthase kinase 3 and protein kinase B/AKT by the integrin-linked kinase. Proc. Natl. Acad. Sci. U.S.A. 1998, 95 (19): 11211–11216. Bibcode:1998PNAS...9511211D. PMC 21621 . PMID 9736715. doi:10.1073/pnas.95.19.11211.