分解肝素与硫酸肝素的酵素通常来自于土壤中的解肝磷脂土地杆菌[52]。解肝磷脂土地杆菌以肝素或硫酸肝素作为它的唯一碳源,为了分解肝素与硫酸肝素,解肝磷脂土地杆菌产生裂解酶,葡糖苷酸酶,磺基酯酶和磺酰胺酶[53] ,最常使用的是裂解酶。解肝磷脂土地杆菌共产生三种裂解酶:肝素酶 I (EC4.2.2.7)、肝素酶 II (没有EC编号)、肝素酶 III (EC4.2.2.8),这三个酵素的专一性详见下表: [54][55]
肝素酶
对受质的专一性(辨认的糖类序列)
肝素酶 I
GlcNS(±6S)-IdoA(2S)
肝素酶 II
GlcNS/Ac(±6S)-IdoA(±2S) GlcNS/Ac(±6S)-GlcA
肝素酶 III
GlcNS/Ac(±6S)-GlcA/IdoA (with a preference for GlcA)
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