HEPES

HEPES（4-(2-羟乙基)-1-哌嗪乙磺酸）是一种两性离子磺酸缓冲剂，它是二十种古德缓冲试剂（英语：Good's buffers）之一，广泛用于细胞培养。^[2]它和1-甲基-3-乙基咪唑𬭩形成离子液体。^[3]

HEPES
IUPAC名 2-[4-(2-Hydroxyethyl)piperazin-1-yl]ethane-1-sulfonic acid
别名	HEPES
识别
CAS号	7365-45-9  Y
PubChem	23831
ChemSpider	22278
SMILES	OCCN1CC[NH+](CCS(=O)([O-])=O)CC1
InChI	1/C8H18N2O4S/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14/h11H,1-8H2,(H,12,13,14)
InChIKey	JKMHFZQWWAIEOD-UHFFFAOYAC
Beilstein	883043
EINECS	230-907-9
ChEBI	42334
RTECS	TL6809000
性质
化学式	C8H18N2O4S
摩尔质量	238.3 g·mol−1
外观	white crystalline powder
密度	Not applicable
熔点	>234-238°C (453-457K)
溶解性（水）	40 g/100 ml (20°C)
pKa	3 (pKa1), 7.5 (pKa2)[1]
危险性
GHS危险性符号
GHS提示词	警告
H-术语	H315, H319, H335
P-术语	P261, P264, P270, P271, P280, P301+312, P302+352, P304+312, P304+340, P305+351+338, P312, P321, P322, P330
主要危害	Eye Irritant
NFPA 704	0 1 1
闪点	Non-flammable
若非注明，所有数据均出自标准状态（25 ℃，100 kPa）下。

参考文献

1. Johnson MA, Seifert S, Petrache HI, Kimble-Hill AC. Phase Coexistence in Single-Lipid Membranes Induced by Buffering Agents. Langmuir. 2014, 30 (33): 9880–9885. PMC 4148158 . doi:10.1021/la5018938.
2. Baicu SC, Taylor MJ. Acid-base buffering in organ preservation solutions as a function of temperature: new parameters for comparing buffer capacity and efficiency. Cryobiology. 2002, 45 (1): 33–48. PMID 12445548. doi:10.1016/S0011-2240(02)00104-9.
3. Mohamed Taha; et al. Good's buffers as a basis for developing self-buffering and biocompatible ionic liquids for biological research. Green Chem., 2014,16, 3149-3159. doi:10.1039/C4GC00328D.