组织蛋白酶D是一种在人体中由CTSD基因编码的蛋白质。[6][7]该基因编码一种溶酶体天冬氨酰蛋白酶，该蛋白酶由二硫键连接的重链和轻链的蛋白二聚体组成，两者均由单一蛋白质前体产生。组织蛋白酶D是一种天冬氨酸内切蛋白酶，广泛分布于溶酶体中。[8]组织蛋白酶D的主要功能是降解蛋白质并激活溶酶体前区室中生物活性蛋白的前体。[9]这种蛋白酶是肽酶A1家族的成员，具有与胃蛋白酶A相似但比胃蛋白酶A更窄的特异性。 CTSD基因的转录从几个位点开始，包括一个作为雌激素调节转录物起始位点的位点。该基因的突变与几种疾病的发病机制有关，包括乳腺癌和可能的阿尔茨海默病症。[7]CTSD基因的纯合缺失导致出生后阶段的早期致死性。[10]据报道，CTSD基因的缺陷是神经元蜡样脂褐质沉积症（NCL）的根本原因。[11]
Quick Facts 组织蛋白酶D, 已知的结构 ...
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组织蛋白酶D的催化位点包括位于14kDa和34kDa链上的两个关键天冬氨酸残基（氨基酸33和231）。[12]成熟组织蛋白酶D的最终形式由337个氨基酸残基、196个重链氨基酸残基和141个轻链氨基酸残基组成。这两条链通过疏水效应连接起来。[13]
在体外，组织蛋白酶D的最适pH值为4.5至5.0。[14]组织蛋白酶D是一种天冬氨酸蛋白酶，它严重依赖于其活性位点Asp残基的质子化。与Asp质子化一起，较低的pH 值也会导致组织蛋白酶D的构象转换：随着pH值的下降，蛋白酶的N端片段会移出活性位点。[15][16][17]与其他天冬氨酸蛋白酶类似，组织蛋白酶D在活性位点的结合裂隙中容纳多达8个氨基酸残基。组织蛋白酶D的主要生理功能包括细胞内蛋白质的代谢降解、多肽激素和生长因子的激活和降解、酶前体的激活、酶激活剂和抑制剂的加工、脑抗原加工和细胞程序性死亡的调节。[18][19][20][21]组织蛋白酶D也可以在细胞外空间中找到，[21]它是少数在中性pH条件下显示出一些活性的组织蛋白酶之一。[22]它能够激活生长因子VEGF-C和VEGF-D，这可能部分解释了它与肿瘤进展的相关性。[23]
NCL表现为视觉功能的进行性丧失和神经发育衰退、癫痫发作、肌阵挛性抽搐和过早死亡。 CTSD基因是已确定的八个基因之一，其缺陷是导致NCL的原因。[11]据报告，外显子6中的纯合单核苷酸重复可以改变阅读框并导致255位的过早终止密码子。组织蛋白酶 D 的过表达刺激致瘤性和转移以及肿瘤细胞凋亡的开始。这种蛋白酶被认为是乳腺癌预后不良的独立标志物，与临床转移的发生率相关。[24][25]CTSD基因敲除会导致肠坏死和出血，胸腺凋亡增加，表明某些上皮细胞需要组织蛋白酶D进行组织重塑和更新。[10]另据报告，CTSD基因型可能对男性阿尔茨海默病风险有强烈影响。[26]组织蛋白酶D的酶活性诱导含有载脂蛋白B-100 的脂蛋白（包括 LDL）的水解修饰，这意味着它也可能与动脉粥样硬化有关。[19][27]
Diment S, Martin KJ, Stahl PD. Cleavage of parathyroid hormone in macrophage endosomes illustrates a novel pathway for intracellular processing of proteins. The Journal of Biological Chemistry. August 1989, 264 (23): 13403–6. PMID 2760027. doi:10.1016/S0021-9258(18)80010-2 
. Saftig P, Hetman M, Schmahl W, Weber K, Heine L, Mossmann H, Köster A, Hess B, Evers M, von Figura K. Mice deficient for the lysosomal proteinase cathepsin D exhibit progressive atrophy of the intestinal mucosa and profound destruction of lymphoid cells. The EMBO Journal. August 1995, 14 (15): 3599–608. PMC 394433 . PMID 7641679. doi:10.1002/j.1460-2075.1995.tb00029.x. Minarowska A, Gacko M, Karwowska A, Minarowski Ł. Human cathepsin D. Folia Histochemica et Cytobiologica / Polish Academy of Sciences, Polish Histochemical and Cytochemical Society. 2008, 46 (1): 23–38. PMID 18296260. doi:10.2478/v10042-008-0003-x . Briozzo P, Morisset M, Capony F, Rougeot C, Rochefort H. In vitro degradation of extracellular matrix with Mr 52,000 cathepsin D secreted by breast cancer cells. Cancer Research. July 1988, 48 (13): 3688–92. PMID 3378211. Authier F, Metioui M, Fabrega S, Kouach M, Briand G. Endosomal proteolysis of internalized insulin at the C-terminal region of the B chain by cathepsin D. The Journal of Biological Chemistry. March 2002, 277 (11): 9437–46. PMID 11779865. doi:10.1074/jbc.M110188200 . Baechle D, Flad T, Cansier A, Steffen H, Schittek B, Tolson J, et al. Cathepsin D is present in human eccrine sweat and involved in the postsecretory processing of the antimicrobial peptide DCD-1L. The Journal of Biological Chemistry. March 2006, 281 (9): 5406–15. PMID 16354654. doi:10.1074/jbc.M504670200 . Hakala JK, Oksjoki R, Laine P, Du H, Grabowski GA, Kovanen PT, Pentikäinen MO. Lysosomal enzymes are released from cultured human macrophages, hydrolyze LDL in vitro, and are present extracellularly in human atherosclerotic lesions. Arteriosclerosis, Thrombosis, and Vascular Biology. August 2003, 23 (8): 1430–6. PMID 12750117. doi:10.1161/01.ATV.0000077207.49221.06 . Bańkowska A, Gacko M, Chyczewska E, Worowska A. Biological and diagnostic role of cathepsin D. Roczniki Akademii Medycznej W Białymstoku. 1997,. 42 Suppl 1: 79–85. PMID 9337526. Lkhider M, Castino R, Bouguyon E, Isidoro C, Ollivier-Bousquet M. Cathepsin D released by lactating rat mammary epithelial cells is involved in prolactin cleavage under physiological conditions. Journal of Cell Science. October 2004, 117 (Pt 21): 5155–64. PMID 15456852. doi:10.1242/jcs.01396 . Jha, Sawan Kumar; Rauniyar, Khushbu; Chronowska, Ewa; Mattonet, Kenny; Maina, Eunice Wairimu; Koistinen, Hannu; Stenman, Ulf-Håkan; Alitalo, Kari; Jeltsch, Michael. KLK3/PSA and cathepsin D activate VEGF-C and VEGF-D. eLife. 2019-05-17, 8: –44478. ISSN 2050-084X. PMC 6588350 . PMID 31099754. doi:10.7554/eLife.44478. Traynor JP, Oun HA, McKenzie P, Shilliday IR, McKay IG, Dunlop A, Geddes CC, Mactier RA. Assessing the utility of the stop dialysate flow method in patients receiving haemodiafiltration. Nephrology, Dialysis, Transplantation. November 2005, 20 (11): 2479–84. PMID 16046508. doi:10.1093/ndt/gfi021 . Menzer G, Müller-Thomsen T, Meins W, Alberici A, Binetti G, Hock C, Nitsch RM, Stoppe G, Reiss J, Finckh U. Non-replication of association between cathepsin D genotype and late onset Alzheimer disease. American Journal of Medical Genetics. March 2001, 105 (2): 179–82. PMID 11304834. doi:10.1002/ajmg.1204. Haidar B, Kiss RS, Sarov-Blat L, Brunet R, Harder C, McPherson R, Marcel YL. Cathepsin D, a lysosomal protease, regulates ABCA1-mediated lipid efflux. The Journal of Biological Chemistry. December 2006, 281 (52): 39971–81. PMID 17032648. doi:10.1074/jbc.M605095200 . Umezawa H, Aoyagi T, Morishima H, Matsuzaki M, Hamada M. Pepstatin, a new pepsin inhibitor produced by Actinomycetes. The Journal of Antibiotics. May 1970, 23 (5): 259–62. PMID 4912600. doi:10.7164/antibiotics.23.259 . Devosse T, Dutoit R, Migeotte I, De Nadai P, Imbault V, Communi D, Salmon I, Parmentier M. Processing of HEBP1 by cathepsin D gives rise to F2L, the agonist of formyl peptide receptor 3. Journal of Immunology. August 2011, 187 (3): 1475–85. PMID 21709160. doi:10.4049/jimmunol.1003545 . Mariani E, Seripa D, Ingegni T, Nocentini G, Mangialasche F, Ercolani S, Cherubini A, Metastasio A, Pilotto A, Senin U, Mecocci P. Interaction of CTSD and A2M polymorphisms in the risk for Alzheimer's disease. Journal of the Neurological Sciences. September 2006, 247 (2): 187–91. PMID 16784755. S2CID 34224448. doi:10.1016/j.jns.2006.05.043. Heinrich M, Wickel M, Schneider-Brachert W, Sandberg C, Gahr J, Schwandner R, Weber T, Saftig P, Peters C, Brunner J, Krönke M, Schütze S. Cathepsin D targeted by acid sphingomyelinase-derived ceramide. The EMBO Journal. October 1999, 18 (19): 5252–63. PMC 1171596 . PMID 10508159. doi:10.1093/emboj/18.19.5252.
- Chao J, Miao RQ, Chen V, Chen LM, Chao L. Novel roles of kallistatin, a specific tissue kallikrein inhibitor, in vascular remodeling. Biological Chemistry. January 2001, 382 (1): 15–21. PMID 11258665. S2CID 33204682. doi:10.1515/BC.2001.003.
- Leto G, Tumminello FM, Crescimanno M, Flandina C, Gebbia N. Cathepsin D expression levels in nongynecological solid tumors: clinical and therapeutic implications. Clinical & Experimental Metastasis. 2004, 21 (2): 91–106. PMID 15168727. S2CID 3476324. doi:10.1023/B:CLIN.0000024740.44602.b7.
- Liaudet-Coopman E, Beaujouin M, Derocq D, Garcia M, Glondu-Lassis M, Laurent-Matha V, Prébois C, Rochefort H, Vignon F. Cathepsin D: newly discovered functions of a long-standing aspartic protease in cancer and apoptosis. Cancer Letters. June 2006, 237 (2): 167–79 [2022-09-18]. PMID 16046058. doi:10.1016/j.canlet.2005.06.007. （原始内容存档于2022-09-21）.
- Knight CG, Barrett AJ. Interaction of human cathepsin D with the inhibitor pepstatin. The Biochemical Journal. April 1976, 155 (1): 117–25. PMC 1172808 . PMID 938470. doi:10.1042/bj1550117.
- Gulnik S, Baldwin ET, Tarasova N, Erickson J. Human liver cathepsin D. Purification, crystallization and preliminary X-ray diffraction analysis of a lysosomal enzyme. Journal of Molecular Biology. September 1992, 227 (1): 265–70 [2022-09-18]. PMID 1522590. doi:10.1016/0022-2836(92)90696-H. （原始内容存档于2022-09-22）.
- Conner GE, Richo G. Isolation and characterization of a stable activation intermediate of the lysosomal aspartyl protease cathepsin D. Biochemistry. February 1992, 31 (4): 1142–7. PMID 1734961. doi:10.1021/bi00119a024.
- Fujita H, Tanaka Y, Noguchi Y, Kono A, Himeno M, Kato K. Isolation and sequencing of a cDNA clone encoding rat liver lysosomal cathepsin D and the structure of three forms of mature enzymes. Biochemical and Biophysical Research Communications. August 1991, 179 (1): 190–6. PMID 1883350. doi:10.1016/0006-291X(91)91353-E.
- Dunn AD, Crutchfield HE, Dunn JT. Thyroglobulin processing by thyroidal proteases. Major sites of cleavage by cathepsins B, D, and L. The Journal of Biological Chemistry. October 1991, 266 (30): 20198–204. PMID 1939080. doi:10.1016/S0021-9258(18)54909-7 .
- Lenarcic B, Krasovec M, Ritonja A, Olafsson I, Turk V. Inactivation of human cystatin C and kininogen by human cathepsin D. FEBS Letters. March 1991, 280 (2): 211–5. PMID 2013314. S2CID 23798502. doi:10.1016/0014-5793(91)80295-E.
- Redecker B, Heckendorf B, Grosch HW, Mersmann G, Hasilik A. Molecular organization of the human cathepsin D gene. DNA and Cell Biology. 1991, 10 (6): 423–31. PMID 2069717. doi:10.1089/dna.1991.10.423.
- Conner GE, Udey JA. Expression and refolding of recombinant human fibroblast procathepsin D. DNA and Cell Biology. 1990, 9 (1): 1–9. PMID 2180427. doi:10.1089/dna.1990.9.1.
- Capony F, Rougeot C, Montcourrier P, Cavailles V, Salazar G, Rochefort H. Increased secretion, altered processing, and glycosylation of pro-cathepsin D in human mammary cancer cells. Cancer Research. July 1989, 49 (14): 3904–9. PMID 2736531.
- Lenarcic B, Kos J, Dolenc I, Lucovnik P, Krizaj I, Turk V. Cathepsin D inactivates cysteine proteinase inhibitors, cystatins. Biochemical and Biophysical Research Communications. July 1988, 154 (2): 765–72. PMID 3261170. doi:10.1016/0006-291X(88)90206-9.
- Westley BR, May FE. Oestrogen regulates cathepsin D mRNA levels in oestrogen responsive human breast cancer cells. Nucleic Acids Research. May 1987, 15 (9): 3773–86. PMC 340781 . PMID 3588310. doi:10.1093/nar/15.9.3773.
- Terayama H, Fukuzumi R. Ubiquitous presence of calciferin-like and cathepsin D-like activities in the sera (vertebrates) and humoral fluids (invertebrates). Comparative Biochemistry and Physiology. B, Comparative Biochemistry. 1987, 87 (4): 675–9. PMID 3665421. doi:10.1016/0305-0491(87)90373-7.
- Sekiguchi K, Siri A, Zardi L, Hakomori S. Differences in domain structure between human fibronectins isolated from plasma and from culture supernatants of normal and transformed fibroblasts. Studies with domain-specific antibodies. The Journal of Biological Chemistry. April 1985, 260 (8): 5105–14. PMID 3988746. doi:10.1016/S0021-9258(18)89185-2 .
- Lemansky P, Gieselmann V, Hasilik A, von Figura K. Cathepsin D and beta-hexosaminidase synthesized in the presence of 1-deoxynojirimycin accumulate in the endoplasmic reticulum. The Journal of Biological Chemistry. August 1984, 259 (16): 10129–35. PMID 6236213. doi:10.1016/S0021-9258(18)90939-7 .
- Dreyer RN, Bausch KM, Fracasso P, Hammond LJ, Wunderlich D, Wirak DO, Davis G, Brini CM, Buckholz TM, König G. Processing of the pre-beta-amyloid protein by cathepsin D is enhanced by a familial Alzheimer's disease mutation. European Journal of Biochemistry. September 1994, 224 (2): 265–71. PMID 7523115. doi:10.1111/j.1432-1033.1994.00265.x .
- Atkins KB, Troen BR. Regulation of cathepsin D gene expression in HL-60 cells by retinoic acid and calcitriol. Cell Growth & Differentiation. July 1995, 6 (7): 871–7. PMID 7547509.
