LIG4

je gen koji kodira proteinsku DNK ligazu IV.^[1] Ovaj protein je ATP zavisna DNK ligaza koja spaja dvolančane prekide tokom nehomolognog spajanja krajeva u putu popravke prekida dvostrukog lanca. On je takođe esencijalan za rekombinaciju.

Ligaza IV, DNK, ATP-zavisna
PDB prikaz baziran na 1ik9​.
Dostupne strukture
1IK9​, 2E2W​, 3II6​
Identifikatori
Simbol	LIG4
Vanjski ID	OMIM: 601837 MGI: 1335098 HomoloGene: 1736 GeneCards: LIG4 Gene
EC broj	6.5.1.1
Ontologija gena Molekularna funkcija • vezivanje nukleotida • DNK vezivanje • aktivnost DNK ligaze • aktivnost DNK ligaze (ATP) • proteinsko vezivanje • ATP vezivanje • vezivanje protein C-terminusa • aktivnost ligaze • vezivanje metalnog jona Celularna komponenta • kondenzovani hromozom • intracelularni • jedro • nukleoplazma • nukleolus • citoplazma • ćelijska membrana • fokalna adhezija • DNK zavisna proteinska kinaza - DNK ligaza 4 kompleks • kompleks DNK ligaze IV • kompleks nehomolognog spajanja krajeva Biološki proces • popravka jednolančanih prekida • in utero embrionski razvoj • pro-B ćelijska diferencijacija • replikacija DNK • ligacija DNK • popravka DNK • popravka dvolančanih prekida
Pregled RNK izražavanja
podaci
Ortolozi
Vrsta	Čovek	Miš
Entrez	3981	319583
Ensembl	ENSG00000174405	ENSMUSG00000049717
UniProt	P49917	Q8BTF5
RefSeq (mRNA)	NM_001098268.1	NM_176953.3
RefSeq (protein)	NP_001091738.1	NP_795927.2
Lokacija (UCSC)	Chr 13: 108.86 - 108.87 Mb	Chr 8: 9.97 - 9.98 Mb
PubMed pretraga

Defekti ovog gena uzrokuju LIG4 sindrom. Lig4 homolog u kvascu je .

Interakcije

formira kompleks sa ,^[2][3] i dalje interaguje sa DNK zavisnom proteinskom kinazom () i .

Reference

1. „Entrez Gene: LIG4 ligase IV, DNA, ATP-dependent”.
2. Deshpande Rajashree A; Wilson Thomas E (2007). „Modes of interaction among yeast Nej1, Lif1 and Dnl4 proteins and comparison to human XLF, XRCC4 and Lig4”. DNA Repair (Amst.). Netherlands. 6 (10): 1507—16. ISSN 1568-7864. PMC 2064958 . PMID 17567543. doi:10.1016/j.dnarep.2007.04.014.
3. Sibanda BL, Critchlow SE, Begun J, Pei XY, Jackson SP, Blundell TL, Pellegrini L (2001). „Crystal structure of an Xrcc4-DNA ligase IV complex”. Nat. Struct. Biol. United States. 8 (12): 1015—9. ISSN 1072-8368. PMID 11702069. doi:10.1038/nsb725.

Literatura

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• Robins P, Lindahl T (1996). „DNA ligase IV from HeLa cell nuclei”. J. Biol. Chem. 271 (39): 24257—61. PMID 8798671. doi:10.1074/jbc.271.39.24257.
• Grawunder U; Wilm M; Wu X; . (1997). „Activity of DNA ligase IV stimulated by complex formation with XRCC4 protein in mammalian cells”. Nature. 388 (6641): 492—5. PMID 9242410. doi:10.1038/41358.
• Critchlow SE, Bowater RP, Jackson SP (1997). „Mammalian DNA double-strand break repair protein XRCC4 interacts with DNA ligase IV”. Curr. Biol. 7 (8): 588—98. PMID 9259561. doi:10.1016/S0960-9822(06)00258-2.
• Grawunder U, Zimmer D, Leiber MR (1998). „DNA ligase IV binds to XRCC4 via a motif located between rather than within its BRCT domains”. Curr. Biol. 8 (15): 873—6. PMID 9705934. doi:10.1016/S0960-9822(07)00349-1.
• Grawunder U; Zimmer D; Fugmann S; . (1998). „DNA ligase IV is essential for V(D)J recombination and DNA double-strand break repair in human precursor lymphocytes”. Mol. Cell. 2 (4): 477—84. PMID 9809069. doi:10.1016/S1097-2765(00)80147-1.
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• Sibanda BL; Critchlow SE; Begun J; . (2002). „Crystal structure of an Xrcc4-DNA ligase IV complex”. Nat. Struct. Biol. 8 (12): 1015—9. PMID 11702069. doi:10.1038/nsb725.
• O'Driscoll M; Cerosaletti KM; Girard PM; . (2002). „DNA ligase IV mutations identified in patients exhibiting developmental delay and immunodeficiency”. Mol. Cell. 8 (6): 1175—85. PMID 11779494. doi:10.1016/S1097-2765(01)00408-7.
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• Mahajan KN, Nick McElhinny SA, Mitchell BS, Ramsden DA (2002). „Association of DNA Polymerase μ (pol μ) with Ku and Ligase IV: Role for pol μ in End-Joining Double-Strand Break Repair”. Mol. Cell. Biol. 22 (14): 5194—202. PMC 139779 . PMID 12077346. doi:10.1128/MCB.22.14.5194-5202.2002.
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• Nicholas C. Price; Lewis Stevens (1999). Fundamentals of Enzymology: The Cell and Molecular Biology of Catalytic Proteins (Third изд.). USA: Oxford University Press. ISBN 019850229X.
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Vidi još

• DNK ligaza

Spoljašnje veze