Saharoza-fosfat sintaza

Saharoza-fosfat sintaza (EC 2.4.1.14, UDP-glukoza fruktoza-fosfat glukoziltransferaza, saharozafosfat UDP glukoziltransferaza, UDP-glukoza-fruktoza-fosfat glukoziltransferaza, SPS, uridin difosfoglukoza-fruktoza fosfat glukoziltransferaza, saharoza 6-fosfat sintaza, saharoza fosfat sintetaza, saharoza fosfat-uridin difosfat glukoziltransferaza, saharoza fosfat sintaza) je enzim sa sistematskim imenom UDP-glukoza:D-fruktoza-6-fosfat 2-alfa-D-glukoziltransferaza.^{[1][2][3][4][5]} Ovaj enzim katalizuje sledeću hemijsku reakciju

UDP-glukoza + D-fruktoza 6-fosfat ${\displaystyle \rightleftharpoons }$ UDP + saharoza 6F-fosfat

Saharoza-fosfat sintaza
Saharoza-fosfat sintaza monomer, Halothermothrix orenii
Identifikatori
EC broj	2.4.1.14
CAS broj	2604333
IntEnz	IntEnz view
BRENDA	BRENDA entry
ExPASy	NiceZyme view
KEGG	KEGG entry
MetaCyc	metabolic pathway
PRIAM	profile
PDB	RCSB PDB PDBe PDBj PDBsum
Pretraga PMC articles PubMed articles NCBI Protein search

Za maksimalno dejstvo ovog enzima je neophodan Mg²⁺ ili Mn²⁺.

Reference

1. Mendicino, J. (1960). „Sucrose phosphate synthesis in wheat germ and green leaves”. J. Biol. Chem. 235: 3347-3352. PMID 13769376.
2. Curatti, L., Folco, E., Desplats, P., Abratti, G., Limones, V., Herrera-Estrella, L. and Salerno, G. (1998). „Sucrose-phosphate synthase from Synechocystis sp. strain PCC 6803: identification of the spsA gene and characterization of the enzyme expressed in Escherichia coli”. J. Bacteriol. 180: 6776-6779. PMID 9852031.
3. Huber, S.C. and Huber, J.L. (1996). „Role and regulation of sucrose-phosphate synthase in higher plants”. Annu. Rev. Plant Physiol. Plant Mol. Biol. 47: 431-444. PMID 15012296.
4. Cumino, A., Curatti, L., Giarrocco, L. and Salerno, G.L. (2002). „Sucrose metabolism: Anabaena sucrose-phosphate synthase and sucrose-phosphate phosphatase define minimal functional domains shuffled during evolution”. FEBS Lett. 517: 19-23. PMID 12062401.
5. Chua, T.K., Bujnicki, J.M., Tan, T.C., Huynh, F., Patel, B.K. and Sivaraman, J. (2008). „The structure of sucrose phosphate synthase from Halothermothrix orenii reveals its mechanism of action and binding mode”. Plant Cell 20: 1059-1072. PMID 18424616.

Literatura

• Nicholas C. Price, Lewis Stevens (1999). Fundamentals of Enzymology: The Cell and Molecular Biology of Catalytic Proteins (Third izd.). USA: Oxford University Press. ISBN 019850229X.
• Eric J. Toone (2006). Advances in Enzymology and Related Areas of Molecular Biology, Protein Evolution (Volume 75 izd.). Wiley-Interscience. ISBN 0471205036.
• Branden C, Tooze J.. Introduction to Protein Structure. New York, NY: Garland Publishing. ISBN: 0-8153-2305-0.
• Irwin H. Segel. Enzyme Kinetics: Behavior and Analysis of Rapid Equilibrium and Steady-State Enzyme Systems (Book 44 izd.). Wiley Classics Library. ISBN 0471303097.
• Robert A. Copeland (2013). Evaluation of Enzyme Inhibitors in Drug Discovery: A Guide for Medicinal Chemists and Pharmacologists (2nd izd.). Wiley-Interscience. ISBN 111848813X.
• Gerhard Michal, Dietmar Schomburg (2012). Biochemical Pathways: An Atlas of Biochemistry and Molecular Biology (2nd izd.). Wiley. ISBN 0470146842.

Spoljašnje veze

• MeSH Sucrose-phosphate+synthase