WIF1

Wnt inhibitory factor 1 is a protein that in humans is encoded by the WIF1 gene.^[5] WIF1 is a lipid-binding protein that binds to Wnt proteins and prevents them from triggering signalling.^[6][7][8]

WIF1
Available structures PDB Ortholog search: PDBe RCSB List of PDB id codes 2D3J, 2YGN, 2YGO, 2YGP, 2YGQ
Identifiers
Aliases	WIF1, WIF-1, WNT inhibitory factor 1
External IDs	OMIM: 605186; MGI: 1344332; HomoloGene: 31430; GeneCards: WIF1; OMA:WIF1 - orthologs
Gene location (Human) Chr. Chromosome 12 (human)[1] Band 12q14.3 Start 65,050,626 bp[1] End 65,121,305 bp[1]
Gene location (Mouse) Chr. Chromosome 10 (mouse)[2] Band 10|10 D2 Start 120,869,865 bp[2] End 120,936,555 bp[2]
RNA expression pattern Bgee Human Mouse (ortholog) Top expressed in lower lobe of lung periodontal fiber tibia parotid gland caudate nucleus putamen corpus epididymis tail of epididymis nucleus accumbens trabecular bone Top expressed in molar skin of external ear dermis body of femur seminal vesicula intercostal muscle trachea ankle calvaria conjunctival fornix More reference expression data BioGPS More reference expression data
Gene ontology Molecular function Wnt-protein binding protein binding signaling receptor binding Cellular component extracellular region cell surface Biological process multicellular organism development negative regulation of Wnt signaling pathway Wnt signaling pathway signal transduction positive regulation of fat cell differentiation anatomical structure development Sources:Amigo / QuickGO
Orthologs Species Human Mouse Entrez 11197 24117 Ensembl ENSG00000156076 ENSMUSG00000020218 UniProt Q9Y5W5 Q9WUA1 RefSeq (mRNA) NM_007191 NM_011915 RefSeq (protein) NP_009122 NP_036045 Location (UCSC) Chr 12: 65.05 – 65.12 Mb Chr 10: 120.87 – 120.94 Mb PubMed search [3] [4]
Wikidata
View/Edit Human View/Edit Mouse

Function

WNT proteins are extracellular signaling molecules involved in the control of embryonic development. This gene encodes a secreted protein, which binds WNT proteins and inhibits their activities. This protein contains a WNT inhibitory factor (WIF) domain and 5 epidermal growth factor (EGF)-like domains. It may be involved in mesoderm segmentation. This protein is found to be present in fish, amphibia and mammals.^[5]

References

1. GRCh38: Ensembl release 89: ENSG00000156076 – Ensembl, May 2017
2. GRCm38: Ensembl release 89: ENSMUSG00000020218 – Ensembl, May 2017
3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
5. "Entrez Gene: WIF1 WNT inhibitory factor 1".
6. Malinauskas T, Aricescu AR, Lu W, Siebold C, Jones EY (July 2011). "Modular mechanism of Wnt signaling inhibition by Wnt inhibitory factor 1". Nature Structural & Molecular Biology. 18 (8): 886–893. doi:10.1038/nsmb.2081. PMC 3430870. PMID 21743455.
7. Malinauskas T (2008). "Docking of fatty acids into the WIF domain of the human Wnt inhibitory factor-1". Lipids. 43 (3): 227–30. doi:10.1007/s11745-007-3144-3. PMID 18256869. S2CID 31357937.
8. Hsieh JC, Kodjabachian L, Rebbert ML, Rattner A, Smallwood PM, Samos CH, Nusse R, Dawid IB, Nathans J (Apr 1999). "A new secreted protein that binds to Wnt proteins and inhibits their activities". Nature. 398 (6726): 431–6. Bibcode:1999Natur.398..431H. doi:10.1038/18899. PMID 10201374. S2CID 4376660.

Further reading

• Shepelev MV, Korobko EV, Korobko IV (2006). "[WIF1: perspectives of application in oncology]". Mol. Gen. Mikrobiol. Virusol. (4): 3–7. PMID 17094650.
• Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. Bibcode:2002PNAS...9916899M. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932.
• Clark HF, Gurney AL, Abaya E, et al. (2003). "The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment". Genome Res. 13 (10): 2265–70. doi:10.1101/gr.1293003. PMC 403697. PMID 12975309.
• Reguart N, He B, Xu Z, et al. (2004). "Cloning and characterization of the promoter of human Wnt inhibitory factor-1". Biochem. Biophys. Res. Commun. 323 (1): 229–34. doi:10.1016/j.bbrc.2004.08.075. PMID 15351726.
• Esufali S, Bapat B (2004). "Cross-talk between Rac1 GTPase and dysregulated Wnt signaling pathway leads to cellular redistribution of beta-catenin and TCF/LEF-mediated transcriptional activation". Oncogene. 23 (50): 8260–71. doi:10.1038/sj.onc.1208007. PMID 15377999. S2CID 30877665.
• Ohigashi T, Mizuno R, Nakashima J, et al. (2005). "Inhibition of Wnt signaling downregulates Akt activity and induces chemosensitivity in PTEN-mutated prostate cancer cells". Prostate. 62 (1): 61–8. doi:10.1002/pros.20117. PMID 15389810. S2CID 1398615.
• Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC 528928. PMID 15489334.
• Simon M, Grandage VL, Linch DC, Khwaja A (2005). "Constitutive activation of the Wnt/beta-catenin signalling pathway in acute myeloid leukaemia". Oncogene. 24 (14): 2410–20. doi:10.1038/sj.onc.1208431. PMID 15735743.
• Taniguchi H, Yamamoto H, Hirata T, et al. (2005). "Frequent epigenetic inactivation of Wnt inhibitory factor-1 in human gastrointestinal cancers". Oncogene. 24 (53): 7946–52. doi:10.1038/sj.onc.1208910. PMID 16007117.
• Lin YC, You L, Xu Z, et al. (2006). "Wnt signaling activation and WIF-1 silencing in nasopharyngeal cancer cell lines". Biochem. Biophys. Res. Commun. 341 (2): 635–40. doi:10.1016/j.bbrc.2005.12.220. PMID 16427602.
• Urakami S, Shiina H, Enokida H, et al. (2006). "Epigenetic inactivation of Wnt inhibitory factor-1 plays an important role in bladder cancer through aberrant canonical Wnt/beta-catenin signaling pathway". Clin. Cancer Res. 12 (2): 383–91. doi:10.1158/1078-0432.CCR-05-1344. PMID 16428476. S2CID 22202251.
• Liepinsh E, Bányai L, Patthy L, Otting G (2006). "NMR structure of the WIF domain of the human Wnt-inhibitory factor-1". J. Mol. Biol. 357 (3): 942–50. doi:10.1016/j.jmb.2006.01.047. PMID 16476441.
• Batra S, Shi Y, Kuchenbecker KM, et al. (2006). "Wnt inhibitory factor-1, a Wnt antagonist, is silenced by promoter hypermethylation in malignant pleural mesothelioma". Biochem. Biophys. Res. Commun. 342 (4): 1228–32. doi:10.1016/j.bbrc.2006.02.084. PMID 16516163.
• Chim CS, Chan WW, Pang A, Kwong YL (2006). "Preferential methylation of Wnt inhibitory factor-1 in acute promyelocytic leukemia: an independent poor prognostic factor". Leukemia. 20 (5): 907–9. doi:10.1038/sj.leu.2404176. PMID 16525492.
• Urakami S, Shiina H, Enokida H, et al. (2006). "Combination analysis of hypermethylated Wnt-antagonist family genes as a novel epigenetic biomarker panel for bladder cancer detection". Clin. Cancer Res. 12 (7 Pt 1): 2109–16. doi:10.1158/1078-0432.CCR-05-2468. PMID 16609023.
• Milutinovic S, D'Alessio AC, Detich N, Szyf M (2007). "Valproate induces widespread epigenetic reprogramming which involves demethylation of specific genes". Carcinogenesis. 28 (3): 560–71. doi:10.1093/carcin/bgl167. PMID 17012225.
• Queimado L, Lopes CS, Reis AM (2007). "WIF1, an inhibitor of the Wnt pathway, is rearranged in salivary gland tumors". Genes Chromosomes Cancer. 46 (3): 215–25. doi:10.1002/gcc.20402. PMID 17171686. S2CID 34184943.
• Chan SL, Cui Y, van Hasselt A, et al. (2007). "The tumor suppressor Wnt inhibitory factor 1 is frequently methylated in nasopharyngeal and esophageal carcinomas". Lab. Invest. 87 (7): 644–50. doi:10.1038/labinvest.3700547. PMID 17384664.
• Veeck J, Wild PJ, Fuchs T, et al. (2009). "Prognostic relevance of Wnt-inhibitory factor-1 (WIF1) and Dickkopf-3 (DKK3) promoter methylation in human breast cancer". BMC Cancer. 9: 1–13. doi:10.1186/1471-2407-9-217. PMC 2717119. PMID 19570204.