UBE2M

NEDD8-conjugating enzyme Ubc12 is a protein that in humans is encoded by the UBE2M gene.^[5][6]

UBE2M
Available structures PDB Ortholog search: PDBe RCSB List of PDB id codes 1TT5, 1Y8X, 2NVU, 3TDU, 3TDZ, 4GAO, 4P5O
Identifiers
Aliases	UBE2M, UBC-RS2, UBC12, hUbc12, ubiquitin conjugating enzyme E2 M
External IDs	OMIM: 603173; MGI: 108278; HomoloGene: 2952; GeneCards: UBE2M; OMA:UBE2M - orthologs
Gene location (Human) Chr. Chromosome 19 (human)[1] Band 19q13.43 Start 58,555,712 bp[1] End 58,558,954 bp[1]
Gene location (Mouse) Chr. Chromosome 7 (mouse)[2] Band 7 A1|7 7.73 cM Start 12,769,047 bp[2] End 12,772,202 bp[2]
RNA expression pattern Bgee Human Mouse (ortholog) Top expressed in right frontal lobe anterior cingulate cortex prefrontal cortex left testis apex of heart anterior pituitary right testis muscle of thigh amygdala right hemisphere of cerebellum Top expressed in superior frontal gyrus muscle of thigh spermatocyte lip spermatid ventricular zone primary visual cortex dentate gyrus of hippocampal formation granule cell right kidney yolk sac More reference expression data BioGPS More reference expression data
Gene ontology Molecular function nucleotide binding protein binding ATP binding ubiquitin protein ligase binding ubiquitin protein ligase activity ubiquitin-protein transferase activity NEDD8 transferase activity transferase activity Cellular component cytoplasm cytosol nucleoplasm Biological process protein neddylation positive regulation of neuron apoptotic process protein ubiquitination post-translational protein modification Sources:Amigo / QuickGO
Orthologs Species Human Mouse Entrez 9040 22192 Ensembl ENSG00000130725 ENSMUSG00000005575 UniProt P61081 P61082 RefSeq (mRNA) NM_003969 NM_001168469 NM_001243968 NM_145578 RefSeq (protein) NP_003960 NP_003960.1 NP_001161941 NP_001230897 NP_663553 Location (UCSC) Chr 19: 58.56 – 58.56 Mb Chr 7: 12.77 – 12.77 Mb PubMed search [3] [4]
Wikidata
View/Edit Human View/Edit Mouse

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. The encoded protein is linked with a ubiquitin-like protein, NEDD8, which can be conjugated to cellular proteins, such as Cdc53/culin.^[6]

Interactions

UBE2M has been shown to interact with NEDD8,^[7] PRKAR1A^[8] and UBA3.^[9]

References

1. GRCh38: Ensembl release 89: ENSG00000130725 – Ensembl, May 2017
2. GRCm38: Ensembl release 89: ENSMUSG00000005575 – Ensembl, May 2017
3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
5. Osaka F, Kawasaki H, Aida N, Saeki M, Chiba T, Kawashima S, Tanaka K, Kato S (Aug 1998). "A new NEDD8-ligating system for cullin-4A". Genes Dev. 12 (15): 2263–8. doi:10.1101/gad.12.15.2263. PMC 317039. PMID 9694792.
6. "Entrez Gene: UBE2M ubiquitin-conjugating enzyme E2M (UBC12 homolog, yeast)".
7. Gong L, Yeh ET (Apr 1999). "Identification of the activating and conjugating enzymes of the NEDD8 conjugation pathway". J. Biol. Chem. 274 (17). UNITED STATES: 12036–42. doi:10.1074/jbc.274.17.12036. ISSN 0021-9258. PMID 10207026.
8. Ewing RM, Chu P, Elisma F, Li H, Taylor P, Climie S, McBroom-Cerajewski L, Robinson MD, O'Connor L, Li M, Taylor R, Dharsee M, Ho Y, Heilbut A, Moore L, Zhang S, Ornatsky O, Bukhman YV, Ethier M, Sheng Y, Vasilescu J, Abu-Farha M, Lambert JP, Duewel HS, Stewart II, Kuehl B, Hogue K, Colwill K, Gladwish K, Muskat B, Kinach R, Adams SL, Moran MF, Morin GB, Topaloglou T, Figeys D (2007). "Large-scale mapping of human protein–protein interactions by mass spectrometry". Mol. Syst. Biol. 3 (1). England: 89. doi:10.1038/msb4100134. PMC 1847948. PMID 17353931.
9. Huang DT, Paydar A, Zhuang M, Waddell MB, Holton JM, Schulman BA (February 2005). "Structural basis for recruitment of Ubc12 by an E2 binding domain in NEDD8's E1". Mol. Cell. 17 (3): 341–50. doi:10.1016/j.molcel.2004.12.020. PMID 15694336.

Further reading

• Gong L, Yeh ET (1999). "Identification of the activating and conjugating enzymes of the NEDD8 conjugation pathway". J. Biol. Chem. 274 (17): 12036–42. doi:10.1074/jbc.274.17.12036. PMID 10207026.
• Chen Y, McPhie DL, Hirschberg J, Neve RL (2000). "The amyloid precursor protein-binding protein APP-BP1 drives the cell cycle through the S-M checkpoint and causes apoptosis in neurons". J. Biol. Chem. 275 (12): 8929–35. doi:10.1074/jbc.275.12.8929. PMID 10722740.
• Wada H, Yeh ET, Kamitani T (2000). "A dominant-negative UBC12 mutant sequesters NEDD8 and inhibits NEDD8 conjugation in vivo". J. Biol. Chem. 275 (22): 17008–15. doi:10.1074/jbc.275.22.17008. PMID 10828074.
• Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. Bibcode:2002PNAS...9916899M. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932.
• Tomsig JL, Snyder SL, Creutz CE (2003). "Identification of targets for calcium signaling through the copine family of proteins. Characterization of a coiled-coil copine-binding motif". J. Biol. Chem. 278 (12): 10048–54. doi:10.1074/jbc.M212632200. PMID 12522145.
• Huang DT, Miller DW, Mathew R, et al. (2004). "A unique E1–E2 interaction required for optimal conjugation of the ubiquitin-like protein NEDD8". Nat. Struct. Mol. Biol. 11 (10): 927–35. doi:10.1038/nsmb826. PMC 2862556. PMID 15361859.
• Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The Status, Quality, and Expansion of the NIH Full-Length cDNA Project: The Mammalian Gene Collection (MGC)". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC 528928. PMID 15489334.
• Huang DT, Paydar A, Zhuang M, et al. (2005). "Structural basis for recruitment of Ubc12 by an E2 binding domain in NEDD8's E1". Mol. Cell. 17 (3): 341–50. doi:10.1016/j.molcel.2004.12.020. PMID 15694336.
• Li T, Santockyte R, Shen RF, et al. (2006). "A general approach for investigating enzymatic pathways and substrates for ubiquitin-like modifiers". Arch. Biochem. Biophys. 453 (1): 70–4. doi:10.1016/j.abb.2006.03.002. PMID 16620772.
• Ewing RM, Chu P, Elisma F, et al. (2007). "Large-scale mapping of human protein–protein interactions by mass spectrometry". Mol. Syst. Biol. 3 (1): 89. doi:10.1038/msb4100134. PMC 1847948. PMID 17353931.