TPH1

Tryptophan hydroxylase 1 (TPH1) is an isoenzyme of tryptophan hydroxylase which in humans is encoded by the TPH1 gene.^[5]

TPH1
Available structures PDB Ortholog search: PDBe RCSB List of PDB id codes 1MLW, 3HF6, 3HF8, 3HFB, 5J6D
Identifiers
Aliases	TPH1, TPRH, TRPH, tryptophan hydroxylase 1
External IDs	OMIM: 191060; MGI: 98796; HomoloGene: 121565; GeneCards: TPH1; OMA:TPH1 - orthologs
Gene location (Human) Chr. Chromosome 11 (human)[1] Band 11p15.1 Start 18,017,555 bp[1] End 18,046,269 bp[1]
Gene location (Mouse) Chr. Chromosome 7 (mouse)[2] Band 7 B3|7 30.43 cM Start 46,294,065 bp[2] End 46,321,961 bp[2]
RNA expression pattern Bgee Human Mouse (ortholog) Top expressed in buccal mucosa cell rectum ascending aorta Descending thoracic aorta mucosa of sigmoid colon testicle pylorus tibial arteries jejunal mucosa duodenum Top expressed in pineal gland duodenum lactiferous gland pyloric antrum embryo embryo jejunum muscle of thigh left colon extraocular muscle More reference expression data BioGPS More reference expression data
Gene ontology Molecular function iron ion binding metal ion binding monooxygenase activity tryptophan 5-monooxygenase activity oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced pteridine as one donor, and incorporation of one atom of oxygen oxidoreductase activity Cellular component cytoplasm cytosol neuron projection Biological process response to immobilization stress mammary gland alveolus development serotonin biosynthetic process negative regulation of ossification aromatic amino acid family metabolic process bone remodeling circadian rhythm positive regulation of fat cell differentiation indolalkylamine biosynthetic process Sources:Amigo / QuickGO
Orthologs Species Human Mouse Entrez 7166 21990 Ensembl ENSG00000129167 ENSMUSG00000040046 UniProt P17752 P17532 RefSeq (mRNA) NM_004179 NM_001136084 NM_001276372 NM_009414 RefSeq (protein) NP_004170 NP_001129556 NP_001263301 NP_033440 Location (UCSC) Chr 11: 18.02 – 18.05 Mb Chr 7: 46.29 – 46.32 Mb PubMed search [3] [4]
Wikidata
View/Edit Human View/Edit Mouse

TPH1 was first discovered to support serotonin synthesis in 1988 by converting tryptophan into 5-hydroxytryptophan.^[6] It was thought that there only was a single TPH gene until 2003. A second form was found in the mouse (Tph2), rat and human brain (TPH2) and the original TPH was then renamed to TPH1.^[7]

Function

Tryptophan hydroxylases catalyze the biopterin-dependent monooxygenation of tryptophan to 5-hydroxytryptophan (5-HTP), which is subsequently decarboxylated by aromatic amino acid decarboxylase to form the neurotransmitter serotonin (5-hydroxytryptamine or 5-HT). It is the rate-limiting enzyme in the biosynthesis of serotonin.

TPH expression is limited to a few specialized tissues: raphe neurons, pinealocytes, mast cells, mononuclear leukocytes, beta-cells of the islets of Langerhans, and intestinal and pancreatic enterochromaffin cells.^{[5][citation needed]}

Clinical significance

Tryptophan hydroxylase is important for synthesizing indoleamine neurotransmitters and related compounds in the body and brain, including serotonin and melatonin. TPH1 is expressed in the body, but not the brain.^[7] Nevertheless, the effect of variations in the TPH1 gene on brain-related variables, such as personality traits and neuropsychiatric disorders, has been studied. For example, one study (1998) found an association between a polymorphism in the gene with impulsive-aggression measures,^[8] while a case-control study (2001) could find no association between polymorphisms and Alzheimer's disease.^[9]

One human mutant of TPH1, A218C found in intron 7, is highly associated with schizophrenia.^[10] Introns are regions of DNA that do not code for the amino acid sequence of a protein and were long considered to be 'junk DNA' lacking purpose. The correlation of an intron mutation with schizophrenia is significant because it suggests that introns have an important role in translation, transcription, or another, possibly unknown, aspect of the production of proteins from DNA.

See also

• Tryptophan hydroxylase
• TPH2
• Rs1799913 (A779C): intron related to figural and numeric creativity
• rs1800532 (A218C)

References

1. GRCh38: Ensembl release 89: ENSG00000129167 – Ensembl, May 2017
2. GRCm38: Ensembl release 89: ENSMUSG00000040046 – Ensembl, May 2017
3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
5. "Entrez Gene: TPH1 tryptophan hydroxylase 1 (tryptophan 5-monooxygenase)".
6. Finocchiaro LM, Arzt ES, Fernández-Castelo S, Criscuolo M, Finkielman S, Nahmod VE (December 1988). "Serotonin and melatonin synthesis in peripheral blood mononuclear cells: stimulation by interferon-gamma as part of an immunomodulatory pathway". J. Interferon Res. 8 (6): 705–16. doi:10.1089/jir.1988.8.705. PMID 3148005.
7. Walther DJ, Peter JU, Bashammakh S, Hörtnagl H, Voits M, Fink H, Bader M (January 2003). "Synthesis of serotonin by a second tryptophan hydroxylase isoform". Science. 299 (5603): 76. doi:10.1126/science.1078197. PMID 12511643. S2CID 7095712.
8. New AS, Gelernter J, Yovell Y, Trestman RL, Nielsen DA, Silverman J, Mitropoulou V, Siever LJ (1998). "Tryptophan hydroxylase genotype is associated with impulsive-aggression measures: a preliminary study". Am. J. Med. Genet. 81 (1): 13–7. doi:10.1002/(SICI)1096-8628(19980207)81:1<13::AID-AJMG3>3.0.CO;2-O. PMID 9514581.
9. Wang YC, Tsai SJ, Liu TY, Liu HC, Hong CJ (January 2001). "No association between tryptophan hydroxylase gene polymorphism and Alzheimer's disease". Neuropsychobiology. 43 (1): 1–4. doi:10.1159/000054856. PMID 11150890. S2CID 39712696.
10. Allen NC, Bagade S, McQueen MB, Ioannidis JP, Kavvoura FK, Khoury MJ, Tanzi RE, Bertram L (July 2008). "Systematic meta-analyses and field synopsis of genetic association studies in schizophrenia: the SzGene database". Nat. Genet. 40 (7): 827–34. doi:10.1038/ng.171. PMID 18583979. S2CID 21772210.

Further reading

• Li D, He L (2007). "Further clarification of the contribution of the tryptophan hydroxylase (TPH) gene to suicidal behavior using systematic allelic and genotypic meta-analyses". Hum. Genet. 119 (3): 233–40. doi:10.1007/s00439-005-0113-x. PMID 16450114. S2CID 28869541.
• Nielsen DA, Dean M, Goldman D (1993). "Genetic mapping of the human tryptophan hydroxylase gene on chromosome 11, using an intronic conformational polymorphism". Am. J. Hum. Genet. 51 (6): 1366–71. PMC 1682899. PMID 1463016.
• Craig SP, Boularand S, Darmon MC, et al. (1991). "Localization of human tryptophan hydroxylase (TPH) to chromosome 11p15.3----p14 by in situ hybridization". Cytogenet. Cell Genet. 56 (3–4): 157–9. doi:10.1159/000133075. PMID 2055111.
• Boularand S, Darmon MC, Ganem Y, et al. (1990). "Complete coding sequence of human tryptophan hydroxylase". Nucleic Acids Res. 18 (14): 4257. doi:10.1093/nar/18.14.4257. PMC 331198. PMID 2377472.
• Ledley FD, Grenett HE, Bartos DP, et al. (1987). "Assignment of human tryptophan hydroxylase locus to chromosome 11: gene duplication and translocation in evolution of aromatic amino acid hydroxylases". Somat. Cell Mol. Genet. 13 (5): 575–80. doi:10.1007/BF01534499. PMID 2889273. S2CID 10999404.
• Ichimura T, Uchiyama J, Kunihiro O, et al. (1996). "Identification of the site of interaction of the 14-3-3 protein with phosphorylated tryptophan hydroxylase". J. Biol. Chem. 270 (48): 28515–8. doi:10.1074/jbc.270.48.28515. PMID 7499362.
• Tipper JP, Citron BA, Ribeiro P, Kaufman S (1995). "Cloning and expression of rabbit and human brain tryptophan hydroxylase cDNA in Escherichia coli". Arch. Biochem. Biophys. 315 (2): 445–53. doi:10.1006/abbi.1994.1523. PMID 7986090.
• Furukawa Y, Ikuta N, Omata S, et al. (1993). "Demonstration of the phosphorylation-dependent interaction of tryptophan hydroxylase with the 14-3-3 protein". Biochem. Biophys. Res. Commun. 194 (1): 144–9. doi:10.1006/bbrc.1993.1796. PMID 8101440.
• Kuhn DM, Arthur R, States JC (1997). "Phosphorylation and activation of brain tryptophan hydroxylase: identification of serine-58 as a substrate site for protein kinase A." J. Neurochem. 68 (5): 2220–3. doi:10.1046/j.1471-4159.1997.68052220.x. PMID 9109552. S2CID 24626272.
• Wang GA, Coon SL, Kaufman S (1998). "Alternative splicing at the 3'-cDNA of human tryptophan hydroxylase". J. Neurochem. 71 (4): 1769–72. doi:10.1046/j.1471-4159.1998.71041769.x. PMID 9751214. S2CID 45630201.
• Austin MC, O'Donnell SM (1999). "Regional distribution and cellular expression of tryptophan hydroxylase messenger RNA in postmortem human brainstem and pineal gland". J. Neurochem. 72 (5): 2065–73. doi:10.1046/j.1471-4159.1999.0722065.x. PMID 10217286. S2CID 43008.
• Yu PL, Fujimura M, Okumiya K, et al. (1999). "Immunohistochemical localization of tryptophan hydroxylase in the human and rat gastrointestinal tracts". J. Comp. Neurol. 411 (4): 654–65. doi:10.1002/(SICI)1096-9861(19990906)411:4<654::AID-CNE9>3.0.CO;2-H. PMID 10421874. S2CID 25930672.
• Kowlessur D, Kaufman S (1999). "Cloning and expression of recombinant human pineal tryptophan hydroxylase in Escherichia coli: purification and characterization of the cloned enzyme". Biochim. Biophys. Acta. 1434 (2): 317–30. doi:10.1016/S0167-4838(99)00184-3. PMID 10525150.
• McKinney J, Teigen K, Frøystein NA, et al. (2002). "Conformation of the substrate and pterin cofactor bound to human tryptophan hydroxylase. Important role of Phe313 in substrate specificity". Biochemistry. 40 (51): 15591–601. doi:10.1021/bi015722x. PMID 11747434.
• Serretti A, Cristina S, Lilli R, et al. (2002). "Family-based association study of 5-HTTLPR, TPH, MAO-A, and DRD4 polymorphisms in mood disorders". Am. J. Med. Genet. 114 (4): 361–9. doi:10.1002/ajmg.10356. PMID 11992558.
• Slominski A, Pisarchik A, Semak I, et al. (2002). "Serotoninergic and melatoninergic systems are fully expressed in human skin". FASEB J. 16 (8): 896–8. doi:10.1096/fj.01-0952fje. PMID 12039872. S2CID 17590408.
• Yohrling IV GJ, Jiang GC, DeJohn MM, et al. (2002). "Inhibition of tryptophan hydroxylase activity and decreased 5-HT1A receptor binding in a mouse model of Huntington's disease". J. Neurochem. 82 (6): 1416–23. doi:10.1046/j.1471-4159.2002.01084.x. PMID 12354289. S2CID 23022076.
• Wang L, Erlandsen H, Haavik J, et al. (2002). "Three-dimensional structure of human tryptophan hydroxylase and its implications for the biosynthesis of the neurotransmitters serotonin and melatonin". Biochemistry. 41 (42): 12569–74. doi:10.1021/bi026561f. PMID 12379098.
• Slominski A, Pisarchik A, Semak I, et al. (2002). "Serotoninergic system in hamster skin". J. Invest. Dermatol. 119 (4): 934–42. doi:10.1046/j.1523-1747.2002.00156.x. PMID 12406341.
• Ono H, Shirakawa O, Kitamura N, et al. (2003). "Tryptophan hydroxylase immunoreactivity is altered by the genetic variation in postmortem brain samples of both suicide victims and controls". Mol. Psychiatry. 7 (10): 1127–32. doi:10.1038/sj.mp.4001150. hdl:20.500.14094/90000364. PMID 12476329.
• Windahl MS, Petersen CR, Christensen HEM, Harris P (2008). "Crystal structure of tryptophan hydroxylase with bound amino acid substrate". Biochemistry. 47 (46): 12087–94. doi:10.1021/bi8015263. PMID 18937498.

External links

• Overview of all the structural information available in the PDB for UniProt: P17752 (Tryptophan 5-hydroxylase 1) at the PDBe-KB.