RNASE1

Ribonuclease pancreatic is an enzyme that in humans is encoded by the RNASE1 gene.^[5][6]

RNASE1
Available structures PDB Ortholog search: PDBe RCSB List of PDB id codes 1DZA, 1E21, 1H8X, 1Z7X, 2E0J, 2E0L, 2E0M, 2E0O, 2K11, 2Q4G, 3F8G, 4KXH
Identifiers
Aliases	RNASE1, RAC1, RIB1, RNS1, ribonuclease A family member 1, pancreatic
External IDs	OMIM: 180440; MGI: 97919; HomoloGene: 7919; GeneCards: RNASE1; OMA:RNASE1 - orthologs
Gene location (Human) Chr. Chromosome 14 (human)[1] Band 14q11.2 Start 20,801,228 bp[1] End 20,802,855 bp[1]
Gene location (Mouse) Chr. Chromosome 14 (mouse)[2] Band 14 C1|14 26.4 cM Start 51,382,455 bp[2] End 51,384,242 bp[2]
RNA expression pattern Bgee Human Mouse (ortholog) Top expressed in right testis left testis right lung upper lobe of left lung synovial joint gallbladder C1 segment body of pancreas decidua synovial membrane Top expressed in vestibular membrane of cochlear duct pyloric antrum islet of Langerhans parotid gland vestibular sensory epithelium crypt of lieberkuhn of small intestine epithelium of stomach stria vascularis esophagus duodenum More reference expression data BioGPS More reference expression data
Gene ontology Molecular function nuclease activity protein binding hydrolase activity ribonuclease A activity nucleic acid binding endonuclease activity ribonuclease activity lyase activity Cellular component extracellular region extracellular exosome Biological process RNA phosphodiester bond hydrolysis, endonucleolytic defense response to virus RNA phosphodiester bond hydrolysis Sources:Amigo / QuickGO
Orthologs Species Human Mouse Entrez 6035 19752 Ensembl ENSG00000129538 ENSMUSG00000035896 UniProt P07998 P00683 Q8C6G3 RefSeq (mRNA) NM_198235 NM_002933 NM_198232 NM_198234 NM_011271 RefSeq (protein) NP_002924 NP_937875 NP_937877 NP_937878 NP_035401 Location (UCSC) Chr 14: 20.8 – 20.8 Mb Chr 14: 51.38 – 51.38 Mb PubMed search [3] [4]
Wikidata
View/Edit Human View/Edit Mouse

Function

This gene encodes a member of the pancreatic-type of secretory ribonucleases, a subset of the ribonuclease A super-family. The encoded endonuclease cleaves internal phosphodiester RNA bonds on the 3'-side of pyrimidine bases. It prefers poly(C) as a substrate and hydrolyses 2',3'-cyclic nucleotides, with a pH optimum near 8.0. The encoded protein is monomeric and more commonly acts to degrade ss-RNA over ds-RNA. Alternative splicing occurs at this locus and four transcript variants encoding the same protein have been identified.^[6]

References

1. GRCh38: Ensembl release 89: ENSG00000129538 – Ensembl, May 2017
2. GRCm38: Ensembl release 89: ENSMUSG00000035896 – Ensembl, May 2017
3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
5. Kochetov AV, Lukasheva VV, Filipenko ML, Mertvetsov NP, Rivkin MI (Sep 1995). "[Primary structure of the coding part of the gene for human pancreatic ribonuclease and its chromosomal location]". Bioorganicheskaia Khimiia. 21 (9): 691–4. PMID 8588814.
6. "Entrez Gene: RNASE1 ribonuclease, RNase A family, 1 (pancreatic)".

Further reading

• Nogués MV, Vilanova M, Cuchillo CM (Nov 1995). "Bovine pancreatic ribonuclease A as a model of an enzyme with multiple substrate binding sites". Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology. 1253 (1): 16–24. doi:10.1016/0167-4838(95)00138-k. PMID 7492594.
• Schienman JE, Holt RA, Auerbach MR, Stewart CB (Aug 2006). "Duplication and divergence of 2 distinct pancreatic ribonuclease genes in leaf-eating African and Asian colobine monkeys". Molecular Biology and Evolution. 23 (8): 1465–79. doi:10.1093/molbev/msl025. PMID 16751256.
• Sakakibara R, Hashida K, Kitahara T, Ishiguro M (Mar 1992). "Characterization of a unique nonsecretory ribonuclease from urine of pregnant women". Journal of Biochemistry. 111 (3): 325–30. doi:10.1093/oxfordjournals.jbchem.a123757. PMID 1587793.
• Haugg M, Schein CH (Feb 1992). "The DNA sequences of the human and hamster secretory ribonucleases determined with the polymerase chain reaction (PCR)". Nucleic Acids Research. 20 (3): 612. doi:10.1093/nar/20.3.612. PMC 310435. PMID 1741299.
• Sakakibara R, Hashida K, Tominaga N, Sakai K, Ishiguro M, Imamura S, Ohmatsu F, Sato E (Jan 1991). "A putative mouse oocyte maturation inhibitory protein from urine of pregnant women: N-terminal sequence homology with human nonsecretory ribonuclease". Chemical & Pharmaceutical Bulletin. 39 (1): 146–9. doi:10.1248/cpb.39.146. PMID 2049798.
• Mizuta K, Awazu S, Yasuda T, Kishi K (Aug 1990). "Purification and characterization of three ribonucleases from human kidney: comparison with urine ribonucleases". Archives of Biochemistry and Biophysics. 281 (1): 144–51. doi:10.1016/0003-9861(90)90424-W. PMID 2383019.
• Beintema JJ, Blank A, Schieven GL, Dekker CA, Sorrentino S, Libonati M (Oct 1988). "Differences in glycosylation pattern of human secretory ribonucleases". The Biochemical Journal. 255 (2): 501–5. PMC 1135256. PMID 3202829.
• Beintema JJ, Wietzes P, Weickmann JL, Glitz DG (Jan 1984). "The amino acid sequence of human pancreatic ribonuclease". Analytical Biochemistry. 136 (1): 48–64. doi:10.1016/0003-2697(84)90306-3. PMID 6201087.
• Russo N, de Nigris M, Ciardiello A, Di Donato A, D'Alessio G (Aug 1995). "Expression in mammalian cells, purification and characterization of recombinant human pancreatic ribonuclease". FEBS Letters. 369 (2–3): 352. doi:10.1016/0014-5793(95)96890-G. PMID 7649283. S2CID 45739736.
• Seno M, Futami J, Kosaka M, Seno S, Yamada H (Aug 1994). "Nucleotide sequence encoding human pancreatic ribonuclease". Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression. 1218 (3): 466–8. doi:10.1016/0167-4781(94)90208-9. PMID 8049276.
• Yasuda T, Nadano D, Takeshita H, Kishi K (Dec 1993). "Two distinct secretory ribonucleases from human cerebrum: purification, characterization and relationships to other ribonucleases". The Biochemical Journal. 296 (3): 617–25. doi:10.1042/bj2960617. PMC 1137742. PMID 8280059.
• Papageorgiou AC, Shapiro R, Acharya KR (Sep 1997). "Molecular recognition of human angiogenin by placental ribonuclease inhibitor--an X-ray crystallographic study at 2.0 A resolution". The EMBO Journal. 16 (17): 5162–77. doi:10.1093/emboj/16.17.5162. PMC 1170149. PMID 9311977.
• Klink TA, Woycechowsky KJ, Taylor KM, Raines RT (Jan 2000). "Contribution of disulfide bonds to the conformational stability and catalytic activity of ribonuclease A". European Journal of Biochemistry. 267 (2): 566–72. doi:10.1046/j.1432-1327.2000.01037.x. PMID 10632727.
• Pous J, Canals A, Terzyan SS, Guasch A, Benito A, Ribó M, Vilanova M, Coll M (Oct 2000). "Three-dimensional structure of a human pancreatic ribonuclease variant, a step forward in the design of cytotoxic ribonucleases". Journal of Molecular Biology. 303 (1): 49–60. doi:10.1006/jmbi.2000.4506. PMID 11021969.
• Pous J, Mallorquí-Fernández G, Peracaula R, Terzyan SS, Futami J, Tada H, Yamada H, Seno M, de Llorens R, Gomis-Rüth FX, Coll M (Apr 2001). "Three-dimensional structure of human RNase 1 delta N7 at 1.9 A resolution" (PDF). Acta Crystallographica Section D. 57 (Pt 4): 498–505. Bibcode:2001AcCrD..57..498P. doi:10.1107/S0907444901001147. PMID 11264578.
• Gaur D, Swaminathan S, Batra JK (Jul 2001). "Interaction of human pancreatic ribonuclease with human ribonuclease inhibitor. Generation of inhibitor-resistant cytotoxic variants". The Journal of Biological Chemistry. 276 (27): 24978–84. doi:10.1074/jbc.M102440200. PMID 11342552.
• Futami J, Maeda T, Kitazoe M, Nukui E, Tada H, Seno M, Kosaka M, Yamada H (Jun 2001). "Preparation of potent cytotoxic ribonucleases by cationization: enhanced cellular uptake and decreased interaction with ribonuclease inhibitor by chemical modification of carboxyl groups". Biochemistry. 40 (25): 7518–24. doi:10.1021/bi010248g. PMID 11412105.
• Canals A, Pous J, Guasch A, Benito A, Ribó M, Vilanova M, Coll M (Oct 2001). "The structure of an engineered domain-swapped ribonuclease dimer and its implications for the evolution of proteins toward oligomerization". Structure. 9 (10): 967–76. doi:10.1016/S0969-2126(01)00659-1. PMID 11591351.

External links

• PDBe-KB provides an overview of all the structure information available in the PDB for Human Ribonuclease pancreatic