RCC2

Protein RCC2 also known as telophase disk protein of 60 kDa (TD-60) or RCC1-like protein TD-60 is a protein that in humans is encoded by the RCC2 gene.^[5][6][7]

RCC2
Identifiers
Aliases	RCC2, TD-60, regulator of chromosome condensation 2
External IDs	OMIM: 609587; MGI: 1919784; HomoloGene: 10282; GeneCards: RCC2; OMA:RCC2 - orthologs
Gene location (Human) Chr. Chromosome 1 (human)[1] Band 1p36.13 Start 17,406,760 bp[1] End 17,439,677 bp[1]
Gene location (Mouse) Chr. Chromosome 4 (mouse)[2] Band 4|4 D3 Start 140,427,852 bp[2] End 140,450,531 bp[2]
RNA expression pattern Bgee Human Mouse (ortholog) Top expressed in ganglionic eminence lymph node monocyte skin of abdomen stromal cell of endometrium ventricular zone skin of leg appendix granulocyte gonad Top expressed in ventricular zone primitive streak somite epiblast thymus abdominal wall fetal liver hematopoietic progenitor cell ganglionic eminence medial ganglionic eminence lip More reference expression data BioGPS n/a
Gene ontology Molecular function protein domain specific binding microtubule binding small GTPase binding protein binding protein kinase binding RNA binding guanyl-nucleotide exchange factor activity Cellular component cytoplasm cytosol early endosome membrane membrane plasma membrane spindle chromosome, centromeric core domain chromosome mitotic spindle midzone midbody nucleolus microtubule chromosome, centromeric region cytoskeleton nucleus Biological process regulation of cell migration activation of GTPase activity establishment of protein localization cell division chromosome passenger complex localization to kinetochore positive regulation of attachment of spindle microtubules to kinetochore focal adhesion assembly negative regulation of GTPase activity regulation of ruffle assembly integrin-mediated signaling pathway positive regulation of G2/M transition of mitotic cell cycle cell cycle negative regulation of substrate adhesion-dependent cell spreading regulation of fibroblast migration negative regulation of focal adhesion assembly sister chromatid cohesion Sources:Amigo / QuickGO
Orthologs Species Human Mouse Entrez 55920 108911 Ensembl ENSG00000281540 ENSG00000179051 ENSMUSG00000040945 UniProt Q9P258 Q8BK67 RefSeq (mRNA) NM_018715 NM_001136204 NM_173867 RefSeq (protein) NP_001129676 NP_061185 NP_001129676.1 NP_061185.1 NP_776292 Location (UCSC) Chr 1: 17.41 – 17.44 Mb Chr 4: 140.43 – 140.45 Mb PubMed search [3] [4]
Wikidata
View/Edit Human View/Edit Mouse

Function

RCC2 has structural similarity to RCC1, and has been shown to have weak guanine nucleotide exchange factor (GEF) activity on the small GTPase Rac.^[6] TD-60 has also been shown to interact with the Chromosomal Passenger Complex (CPC), and to activate the catalytic component of the CPC, Aurora B, in the presence of microtubules.^[8][9]

References

1. ENSG00000179051 GRCh38: Ensembl release 89: ENSG00000281540, ENSG00000179051 – Ensembl, May 2017
2. GRCm38: Ensembl release 89: ENSMUSG00000040945 – Ensembl, May 2017
3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
5. Nagase T, Kikuno R, Ishikawa K, Hirosawa M, Ohara O (Sep 2000). "Prediction of the coding sequences of unidentified human genes. XVII. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro". DNA Res. 7 (2): 143–50. doi:10.1093/dnares/7.2.143. PMID 10819331.
6. Mollinari C, Reynaud C, Martineau-Thuillier S, Monier S, Kieffer S, Garin J, Andreassen PR, Boulet A, Goud B, Kleman JP, Margolis RL (Aug 2003). "The mammalian passenger protein TD-60 is an RCC1 family member with an essential role in prometaphase to metaphase progression". Dev Cell. 5 (2): 295–307. doi:10.1016/S1534-5807(03)00205-3. PMID 12919680.
7. "Entrez Gene: RCC2 regulator of chromosome condensation 2".
8. Gassmann R, Carvalho A, Henzing AJ, Ruchaud S, Hudson DF, Honda R, Nigg EA, Gerloff DL, Earnshaw WC (July 2004). "Borealin: a novel chromosomal passenger required for stability of the bipolar mitotic spindle". J. Cell Biol. 166 (2): 179–91. doi:10.1083/jcb.200404001. PMC 2172304. PMID 15249581.
9. Rosasco-Nitcher SE, Lan W, Khorasanizadeh S, Stukenberg PT (January 2008). "Centromeric Aurora-B activation requires TD-60, microtubules, and substrate priming phosphorylation". Science. 319 (5862): 469–72. Bibcode:2008Sci...319..469R. doi:10.1126/science.1148980. PMID 18218899. S2CID 10374348.

Further reading

• Andreassen PR, Palmer DK, Wener MH, Margolis RL (1991). "Telophase disc: a new mammalian mitotic organelle that bisects telophase cells with a possible function in cytokinesis". J. Cell Sci. 99 (3): 523–34. doi:10.1242/jcs.99.3.523. PMID 1939370.
• Martineau SN, Andreassen PR, Margolis RL (1995). "Delay of HeLa cell cleavage into interphase using dihydrocytochalasin B: retention of a postmitotic spindle and telophase disc correlates with synchronous cleavage recovery". J. Cell Biol. 131 (1): 191–205. doi:10.1083/jcb.131.1.191. PMC 2120587. PMID 7559776.
• Martineau-Thuillier S, Andreassen PR, Margolis RL (1999). "Colocalization of TD-60 and INCENP throughout G2 and mitosis: evidence for their possible interaction in signalling cytokinesis". Chromosoma. 107 (6–7): 461–70. doi:10.1007/s004120050330. PMID 9914378. S2CID 24935187.
• Scherl A, Couté Y, Déon C, et al. (2003). "Functional proteomic analysis of human nucleolus". Mol. Biol. Cell. 13 (11): 4100–9. doi:10.1091/mbc.E02-05-0271. PMC 133617. PMID 12429849.
• Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. Bibcode:2002PNAS...9916899M. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932.
• Gassmann R, Carvalho A, Henzing AJ, et al. (2004). "Borealin: a novel chromosomal passenger required for stability of the bipolar mitotic spindle". J. Cell Biol. 166 (2): 179–91. doi:10.1083/jcb.200404001. PMC 2172304. PMID 15249581.
• Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC 528928. PMID 15489334.
• Olsen JV, Blagoev B, Gnad F, et al. (2006). "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks". Cell. 127 (3): 635–48. doi:10.1016/j.cell.2006.09.026. PMID 17081983. S2CID 7827573.
• Rosasco-Nitcher, Sara; Lan W; Khorasanizadeh S; Stukenberg PT. (January 2008). "Centromeric Aurora-B activation requires TD-60, microtubules, and substrate priming phosphorylation". Science. 319 (5862): 469–472. Bibcode:2008Sci...319..469R. doi:10.1126/science.1148980. PMID 18218899. S2CID 10374348.

External links

• Overview of all the structural information available in the PDB for UniProt: Q9P258 (Protein RCC2) at the PDBe-KB.