POLR2F

DNA-directed RNA polymerases I, II, and III subunit RPABC2 is a protein that in humans is encoded by the POLR2F gene.^[5]

POLR2F
Available structures PDB Ortholog search: PDBe RCSB List of PDB id codes 1QKL, 5FLM, 5IY9, 5IYA, 5IYC, 5IYB, 5IY7, 5IY8, 5IYD, 5IY6
Identifiers
Aliases	POLR2F, HRBP14.4, POLRF, RPABC14.4, RPABC2, RPB14.4, RPB6, RPC15, polymerase (RNA) II subunit F, RNA polymerase II subunit F, RNA polymerase II, I and III subunit F
External IDs	OMIM: 604414; MGI: 1349393; HomoloGene: 7178; GeneCards: POLR2F; OMA:POLR2F - orthologs
Gene location (Human) Chr. Chromosome 22 (human)[1] Band 22q13.1 Start 37,952,607 bp[1] End 38,041,915 bp[1]
Gene location (Mouse) Chr. Chromosome 15 (mouse)[2] Band 15 E1|15 37.7 cM Start 79,025,209 bp[2] End 79,035,974 bp[2]
RNA expression pattern Bgee Human Mouse (ortholog) Top expressed in C1 segment tibial nerve tendon of biceps brachii apex of heart right lobe of liver right auricle amygdala left ventricle anterior pituitary ganglionic eminence Top expressed in yolk sac morula morula otic vesicle medial ganglionic eminence endothelial cell of lymphatic vessel embryo embryo ventricular zone vestibular membrane of cochlear duct More reference expression data BioGPS More reference expression data
Gene ontology Molecular function DNA binding RNA polymerase II activity RNA polymerase III activity DNA-directed 5'-3' RNA polymerase activity RNA polymerase I activity Cellular component cytosol nucleoplasm RNA polymerase I complex RNA polymerase III complex RNA polymerase II, core complex nucleus fibrillar center Biological process termination of RNA polymerase I transcription mRNA splicing, via spliceosome epigenetic maintenance of chromatin in transcription-competent conformation transcription initiation from RNA polymerase I promoter transcription elongation from RNA polymerase II promoter 7-methylguanosine mRNA capping transcription by RNA polymerase II transcription-coupled nucleotide-excision repair transcription initiation from RNA polymerase II promoter snRNA transcription by RNA polymerase II fibroblast growth factor receptor signaling pathway transcription by RNA polymerase III RNA metabolic process regulation of gene silencing by miRNA transcription, DNA-templated transcription elongation from RNA polymerase I promoter positive regulation of type I interferon production somatic stem cell population maintenance positive regulation of viral transcription Sources:Amigo / QuickGO
Orthologs Species Human Mouse Entrez 5435 69833 Ensembl ENSG00000100142 ENSMUSG00000033020 UniProt P61218 P61219 RefSeq (mRNA) NM_001301129 NM_001301130 NM_001301131 NM_021974 NM_001363825 NM_027231 RefSeq (protein) NP_001288058 NP_001288059 NP_001288060 NP_068809 NP_001350754 NP_081507 Location (UCSC) Chr 22: 37.95 – 38.04 Mb Chr 15: 79.03 – 79.04 Mb PubMed search [3] [4]
Wikidata
View/Edit Human View/Edit Mouse

This gene encodes the sixth largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes, that is also shared by the other two DNA-directed RNA polymerases. In yeast, this polymerase subunit, in combination with at least two other subunits, forms a structure that stabilizes the transcribing polymerase on the DNA template.^[6]

Interactions

POLR2F has been shown to interact with POLR2C.^[7]

See also

• RNA polymerase II

References

1. GRCh38: Ensembl release 89: ENSG00000100142 – Ensembl, May 2017
2. GRCm38: Ensembl release 89: ENSMUSG00000033020 – Ensembl, May 2017
3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
5. Pusch C, Wang Z, Roe B, et al. (September 1996). "Genomic structure of the RNA polymerase II small subunit (hRPB14.4) locus (POLRF) and mapping to 22q13.1 by sequence identity". Genomics. 34 (3): 440–2. doi:10.1006/geno.1996.0312. PMID 8786150.
6. "Entrez Gene: POLR2F polymerase (RNA) II (DNA directed) polypeptide F".
7. Acker J, de Graaff M, Cheynel I, et al. (July 1997). "Interactions between the human RNA polymerase II subunits". J. Biol. Chem. 272 (27). UNITED STATES: 16815–21. doi:10.1074/jbc.272.27.16815. ISSN 0021-9258. PMID 9201987.

Further reading

• Jeang KT (1998). "Tat, Tat-associated kinase, and transcription". J. Biomed. Sci. 5 (1): 24–7. doi:10.1007/BF02253352. PMID 9570510.
• Yankulov K, Bentley D (1998). "Transcriptional control: Tat cofactors and transcriptional elongation". Curr. Biol. 8 (13): R447–9. Bibcode:1998CBio....8.R447Y. doi:10.1016/S0960-9822(98)70289-1. PMID 9651670.
• Romano G, Kasten M, De Falco G, et al. (2000). "Regulatory functions of Cdk9 and of cyclin T1 in HIV tat transactivation pathway gene expression". J. Cell. Biochem. 75 (3): 357–68. doi:10.1002/(SICI)1097-4644(19991201)75:3<357::AID-JCB1>3.0.CO;2-K. PMID 10536359. S2CID 43685090.
• Marcello A, Zoppé M, Giacca M (2002). "Multiple modes of transcriptional regulation by the HIV-1 Tat transactivator". IUBMB Life. 51 (3): 175–81. doi:10.1080/152165401753544241. PMID 11547919. S2CID 10931640.
• Stevens M, De Clercq E, Balzarini J (2007). "The regulation of HIV-1 transcription: molecular targets for chemotherapeutic intervention". Med Res Rev. 26 (5): 595–625. doi:10.1002/med.20081. PMC 7168390. PMID 16838299.
• Harrich D, McMillan N, Munoz L, et al. (2007). "Will diverse Tat interactions lead to novel antiretroviral drug targets?". Current Drug Targets. 7 (12): 1595–606. doi:10.2174/138945006779025338. PMID 17168834.
• Kato H, Sumimoto H, Pognonec P, et al. (1992). "HIV-1 Tat acts as a processivity factor in vitro in conjunction with cellular elongation factors". Genes Dev. 6 (4): 655–66. doi:10.1101/gad.6.4.655. PMID 1559613.
• Southgate C, Zapp ML, Green MR (1990). "Activation of transcription by HIV-1 Tat protein tethered to nascent RNA through another protein". Nature. 345 (6276): 640–2. Bibcode:1990Natur.345..640S. doi:10.1038/345640a0. PMID 2190099. S2CID 4233742.
• Wu-Baer F, Sigman D, Gaynor RB (1995). "Specific binding of RNA polymerase II to the human immunodeficiency virus trans-activating region RNA is regulated by cellular cofactors and Tat". Proc. Natl. Acad. Sci. U.S.A. 92 (16): 7153–7. Bibcode:1995PNAS...92.7153W. doi:10.1073/pnas.92.16.7153. PMC 41297. PMID 7638159.
• Acker J, Wintzerith M, Vigneron M, et al. (1995). "A 14.4 KDa acidic subunit of human RNA polymerase II with a putative leucine-zipper". DNA Seq. 4 (5): 329–31. doi:10.3109/10425179409020860. PMID 7803819.
• Herrmann CH, Rice AP (1995). "Lentivirus Tat proteins specifically associate with a cellular protein kinase, TAK, that hyperphosphorylates the carboxyl-terminal domain of the large subunit of RNA polymerase II: candidate for a Tat cofactor". J. Virol. 69 (3): 1612–20. doi:10.1128/JVI.69.3.1612-1620.1995. PMC 188757. PMID 7853496.
• Keen NJ, Gait MJ, Karn J (1996). "Human immunodeficiency virus type-1 Tat is an integral component of the activated transcription-elongation complex". Proc. Natl. Acad. Sci. U.S.A. 93 (6): 2505–10. Bibcode:1996PNAS...93.2505K. doi:10.1073/pnas.93.6.2505. PMC 39827. PMID 8637904.
• Yang X, Herrmann CH, Rice AP (1996). "The human immunodeficiency virus Tat proteins specifically associate with TAK in vivo and require the carboxyl-terminal domain of RNA polymerase II for function". J. Virol. 70 (7): 4576–84. doi:10.1128/JVI.70.7.4576-4584.1996. PMC 190394. PMID 8676484.
• Agostini I, Navarro JM, Rey F, et al. (1996). "The human immunodeficiency virus type 1 Vpr transactivator: cooperation with promoter-bound activator domains and binding to TFIIB". J. Mol. Biol. 261 (5): 599–606. doi:10.1006/jmbi.1996.0485. PMID 8800208.
• Zhou Q, Sharp PA (1996). "Tat-SF1: cofactor for stimulation of transcriptional elongation by HIV-1 Tat". Science. 274 (5287): 605–10. Bibcode:1996Sci...274..605Z. doi:10.1126/science.274.5287.605. PMID 8849451. S2CID 13266489.
• Okamoto H, Sheline CT, Corden JL, et al. (1996). "Trans-activation by human immunodeficiency virus Tat protein requires the C-terminal domain of RNA polymerase II". Proc. Natl. Acad. Sci. U.S.A. 93 (21): 11575–9. Bibcode:1996PNAS...9311575O. doi:10.1073/pnas.93.21.11575. PMC 38099. PMID 8876177.
• Chun RF, Jeang KT (1996). "Requirements for RNA polymerase II carboxyl-terminal domain for activated transcription of human retroviruses human T-cell lymphotropic virus I and HIV-1". J. Biol. Chem. 271 (44): 27888–94. doi:10.1074/jbc.271.44.27888. PMID 8910388.
• Parada CA, Roeder RG (1996). "Enhanced processivity of RNA polymerase II triggered by Tat-induced phosphorylation of its carboxy-terminal domain". Nature. 384 (6607): 375–8. Bibcode:1996Natur.384..375P. doi:10.1038/384375a0. PMID 8934526. S2CID 4278432.
• García-Martínez LF, Ivanov D, Gaynor RB (1997). "Association of Tat with purified HIV-1 and HIV-2 transcription preinitiation complexes". J. Biol. Chem. 272 (11): 6951–8. doi:10.1074/jbc.272.11.6951. PMID 9054383.