POLD3

DNA polymerase delta subunit 3 is an enzyme that in humans is encoded by the POLD3 gene.^[5][6] It is a component of the DNA polymerase delta complex.

POLD3
Available structures PDB Ortholog search: PDBe RCSB List of PDB id codes 1U76, 3E0J, 2N1G
Identifiers
Aliases	POLD3, P66, P68, PPP1R128, polymerase (DNA) delta 3, accessory subunit, DNA polymerase delta 3, accessory subunit
External IDs	OMIM: 611415; MGI: 1915217; HomoloGene: 38202; GeneCards: POLD3; OMA:POLD3 - orthologs
Gene location (Human) Chr. Chromosome 11 (human)[1] Band 11q13.4 Start 74,493,851 bp[1] End 74,669,117 bp[1]
Gene location (Mouse) Chr. Chromosome 7 (mouse)[2] Band 7|7 E2 Start 99,731,318 bp[2] End 99,770,772 bp[2]
RNA expression pattern Bgee Human Mouse (ortholog) Top expressed in secondary oocyte ventricular zone testicle sperm ganglionic eminence rectum gonad monocyte kidney tubule bronchial epithelial cell Top expressed in secondary oocyte zygote tail of embryo primary oocyte genital tubercle spermatid morula somite primitive streak seminiferous tubule More reference expression data BioGPS More reference expression data
Gene ontology Molecular function protein binding DNA-directed DNA polymerase activity Cellular component delta DNA polymerase complex nucleus nucleoplasm cytoplasm Biological process nucleotide-excision repair, DNA gap filling DNA synthesis involved in DNA repair DNA replication translesion synthesis transcription-coupled nucleotide-excision repair nucleotide-excision repair, DNA incision telomere maintenance DNA-dependent DNA replication nucleotide-excision repair, DNA incision, 5'-to lesion DNA repair cellular response to DNA damage stimulus telomere maintenance via semi-conservative replication DNA mismatch repair DNA strand elongation involved in DNA replication DNA synthesis involved in UV-damage excision repair Sources:Amigo / QuickGO
Orthologs Species Human Mouse Entrez 10714 67967 Ensembl ENSG00000077514 ENSMUSG00000030726 UniProt Q15054 Q9EQ28 RefSeq (mRNA) NM_006591 NM_001363597 NM_133692 NM_001368380 RefSeq (protein) NP_006582 NP_001350526 NP_598453 NP_001355309 Location (UCSC) Chr 11: 74.49 – 74.67 Mb Chr 7: 99.73 – 99.77 Mb PubMed search [3] [4]
Wikidata
View/Edit Human View/Edit Mouse

Interactions

POLD3 has been shown to interact with PCNA.^[7][8]

References

1. GRCh38: Ensembl release 89: ENSG00000077514 – Ensembl, May 2017
2. GRCm38: Ensembl release 89: ENSMUSG00000030726 – Ensembl, May 2017
3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
5. Hughes P, Tratner I, Ducoux M, Piard K, Baldacci G (Jun 1999). "Isolation and identification of the third subunit of mammalian DNA polymerase delta by PCNA-affinity chromatography of mouse FM3A cell extracts". Nucleic Acids Res. 27 (10): 2108–14. doi:10.1093/nar/27.10.2108. PMC 148430. PMID 10219083.
6. "Entrez Gene: POLD3 polymerase (DNA-directed), delta 3, accessory subunit".
7. Ducoux M, Urbach S, Baldacci G, Hübscher U, Koundrioukoff S, Christensen J, Hughes P (Dec 2001). "Mediation of proliferating cell nuclear antigen (PCNA)-dependent DNA replication through a conserved p21(Cip1)-like PCNA-binding motif present in the third subunit of human DNA polymerase delta". J. Biol. Chem. 276 (52): 49258–66. doi:10.1074/jbc.M106990200. PMID 11595739.
8. Ohta S, Shiomi Y, Sugimoto K, Obuse C, Tsurimoto T (Oct 2002). "A proteomics approach to identify proliferating cell nuclear antigen (PCNA)-binding proteins in human cell lysates. Identification of the human CHL12/RFCs2-5 complex as a novel PCNA-binding protein". J. Biol. Chem. 277 (43): 40362–7. doi:10.1074/jbc.M206194200. PMID 12171929.

Further reading

• Nomura N, Miyajima N, Sazuka T, Tanaka A, Kawarabayasi Y, Sato S, Nagase T, Seki N, Ishikawa K, Tabata S (1995). "Prediction of the coding sequences of unidentified human genes. I. The coding sequences of 40 new genes (KIAA0001-KIAA0040) deduced by analysis of randomly sampled cDNA clones from human immature myeloid cell line KG-1". DNA Res. 1 (1): 27–35. doi:10.1093/dnares/1.1.27. PMID 7584026.
• Zeng XR, Jiang Y, Zhang SJ, Hao H, Lee MY (1994). "DNA polymerase delta is involved in the cellular response to UV damage in human cells". J. Biol. Chem. 269 (19): 13748–51. doi:10.1016/S0021-9258(17)36709-1. PMID 7910606.
• Longley MJ, Pierce AJ, Modrich P (1997). "DNA polymerase delta is required for human mismatch repair in vitro". J. Biol. Chem. 272 (16): 10917–21. doi:10.1074/jbc.272.16.10917. PMID 9099749.
• Mo J, Liu L, Leon A, Mazloum N, Lee MY (2000). "Evidence that DNA polymerase delta isolated by immunoaffinity chromatography exhibits high-molecular weight characteristics and is associated with the KIAA0039 protein and RPA". Biochemistry. 39 (24): 7245–54. doi:10.1021/bi0000871. PMID 10852724.
• Ducoux M, Urbach S, Baldacci G, Hübscher U, Koundrioukoff S, Christensen J, Hughes P (2002). "Mediation of proliferating cell nuclear antigen (PCNA)-dependent DNA replication through a conserved p21(Cip1)-like PCNA-binding motif present in the third subunit of human DNA polymerase delta". J. Biol. Chem. 276 (52): 49258–66. doi:10.1074/jbc.M106990200. PMID 11595739.
• Ohta S, Shiomi Y, Sugimoto K, Obuse C, Tsurimoto T (2002). "A proteomics approach to identify proliferating cell nuclear antigen (PCNA)-binding proteins in human cell lysates. Identification of the human CHL12/RFCs2-5 complex as a novel PCNA-binding protein". J. Biol. Chem. 277 (43): 40362–7. doi:10.1074/jbc.M206194200. PMID 12171929.
• Pohler JR, Otterlei M, Warbrick E (2006). "An in vivo analysis of the localisation and interactions of human p66 DNA polymerase delta subunit". BMC Mol. Biol. 6: 17. doi:10.1186/1471-2199-6-17. PMC 1187890. PMID 16000169.
• Takemura M, Yoshida S, Akiyama T, Kitagawa M, Yamada Y (2006). "Role of the second-largest subunit of DNA polymerase alpha in the interaction between the catalytic subunit and hyperphosphorylated retinoblastoma protein in late S phase". Biochim. Biophys. Acta. 1764 (9): 1447–53. doi:10.1016/j.bbapap.2006.06.015. PMID 16935576.
• Olsen JV, Blagoev B, Gnad F, Macek B, Kumar C, Mortensen P, Mann M (2006). "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks". Cell. 127 (3): 635–48. doi:10.1016/j.cell.2006.09.026. PMID 17081983. S2CID 7827573.
• Masuda Y, Suzuki M, Piao J, Gu Y, Tsurimoto T, Kamiya K (2007). "Dynamics of human replication factors in the elongation phase of DNA replication". Nucleic Acids Res. 35 (20): 6904–16. doi:10.1093/nar/gkm822. PMC 2175312. PMID 17932049.