For protein methods, see Protein methods.Nucleic acid methods are the techniques used to study nucleic acids: DNA and RNA. Purification DNA extraction Phenol–chloroform extraction Minicolumn purification RNA extraction Boom method Synchronous coefficient of drag alteration (SCODA) DNA purification Quantification Abundance in weight: spectroscopic nucleic acid quantitation Absolute abundance in number: real-time polymerase chain reaction (quantitative PCR) High-throughput relative abundance: DNA microarray High-throughput absolute abundance: serial analysis of gene expression (SAGE) Size: gel electrophoresis Synthesis De novo: oligonucleotide synthesis Amplification: polymerase chain reaction (PCR), loop-mediated isothermal amplification (LAMP), transcription-mediated amplification (TMA) Kinetics Multi-parametric surface plasmon resonance[1][2] Dual-polarization interferometry[3] Quartz crystal microbalance with dissipation monitoring (QCM-D)[4] Gene function RNA interference[5] Other Bisulfite sequencing DNA sequencing Expression cloning Fluorescence in situ hybridization Lab-on-a-chip Comparison of nucleic acid simulation software Northern blot Nuclear run-on assay Radioactivity in the life sciences Southern blot Differential centrifugation (sucrose gradient) Toeprinting assay Several bioinformatics methods, as seen in list of RNA structure prediction software See also CSH Protocols Current Protocols References [1]Tang, Wei; Hu, Shichao; Wang, Huaming; Zhao, Yan; Li, Na; Liu, Feng (23 September 2014). "A universal molecular translator for non-nucleic acid targets that enables dynamic DNA assemblies and logic operations". Chem. Commun. 50 (92): 14352–14355. doi:10.1039/C4CC07041K. PMID 25295484. [2]Ihalainen, Petri; Pettersson, Fredrik; Pesonen, Markus; Viitala, Tapani; Määttänen, Anni; Österbacka, Ronald; Peltonen, Jouko (7 March 2014). "An impedimetric study of DNA hybridization on paper-supported inkjet-printed gold electrodes". Nanotechnology. 25 (9): 094009. Bibcode:2014Nanot..25i4009I. doi:10.1088/0957-4484/25/9/094009. PMID 24522116. S2CID 206070778. [3]Berney, H.; Oliver, K. (2005). "Dual polarization interferometry size and density characterisation of DNA immobilisation and hybridisation". Biosensors and Bioelectronics. 21 (4): 618–626. doi:10.1016/j.bios.2004.12.024. PMID 16202875. [4]Dixon, Matthew C. (July 2008). "Quartz Crystal Microbalance with Dissipation Monitoring: Enabling Real-Time Characterization of Biological Materials and Their Interactions". Journal of Biomolecular Techniques. 19 (3): 151–158. PMC 2563918. PMID 19137101. [5]Hannon, Gregory J. (July 2002). "RNA interference". Nature. 418 (6894): 244–251. Bibcode:2002Natur.418..244H. doi:10.1038/418244a. ISSN 1476-4687. PMID 12110901. S2CID 4426281. External links Protocols for Recombinant DNA Isolation, Cloning, and SequencingWikiwand - on Seamless Wikipedia browsing. On steroids.