NCK2

Cytoplasmic protein NCK2 (also known as NCK-beta and Grb4) is a protein that in humans is encoded by the NCK2 gene.^[5][6][7]

NCK2
Available structures PDB Ortholog search: PDBe RCSB List of PDB id codes 1U5S, 1WX6, 1Z3K, 2B86, 2CIA, 2FRW, 2FRY, 2JXB, 4E6R
Identifiers
Aliases	NCK2, GRB4, NCKbeta, NCK adaptor protein 2
External IDs	OMIM: 604930; MGI: 1306821; HomoloGene: 20794; GeneCards: NCK2; OMA:NCK2 - orthologs
Gene location (Human) Chr. Chromosome 2 (human)[1] Band 2q12.2 Start 105,744,912 bp[1] End 105,894,274 bp[1]
Gene location (Mouse) Chr. Chromosome 1 (mouse)[2] Band 1|1 C1.1 Start 43,483,739 bp[2] End 43,609,675 bp[2]
RNA expression pattern Bgee Human Mouse (ortholog) Top expressed in hair follicle parotid gland secondary oocyte Region I of hippocampus proper ganglionic eminence cartilage tissue duodenum olfactory zone of nasal mucosa gums gastric mucosa Top expressed in dentate gyrus of hippocampal formation granule cell ventricular zone thymus lip tail of embryo yolk sac primary visual cortex spermatid right kidney genital tubercle More reference expression data BioGPS n/a
Gene ontology Molecular function scaffold protein binding protein binding cytoskeletal anchor activity signaling receptor complex adaptor activity phosphotyrosine residue binding protein-containing complex binding Cellular component cytosol vesicle membrane synapse endoplasmic reticulum cytoplasm postsynaptic density Biological process positive regulation of actin filament polymerization epidermal growth factor receptor signaling pathway positive regulation of endoplasmic reticulum stress-induced intrinsic apoptotic signaling pathway actin filament organization positive regulation of translation in response to endoplasmic reticulum stress ephrin receptor signaling pathway signal complex assembly dendritic spine development vascular endothelial growth factor receptor signaling pathway positive regulation of T cell proliferation negative regulation of peptidyl-serine phosphorylation regulation of epidermal growth factor-activated receptor activity negative regulation of endoplasmic reticulum stress-induced eIF2 alpha phosphorylation immunological synapse formation cell migration negative regulation of PERK-mediated unfolded protein response negative regulation of transcription from RNA polymerase II promoter in response to endoplasmic reticulum stress T cell activation regulation of translation lamellipodium assembly signal transduction negative regulation of cell population proliferation positive regulation of transcription by RNA polymerase II Sources:Amigo / QuickGO
Orthologs Species Human Mouse Entrez 8440 17974 Ensembl ENSG00000071051 ENSMUSG00000066877 UniProt O43639 O55033 RefSeq (mRNA) NM_001004720 NM_001004722 NM_003581 NM_010879 RefSeq (protein) NP_001004720 NP_001004722 NP_003572 NP_035009 Location (UCSC) Chr 2: 105.74 – 105.89 Mb Chr 1: 43.48 – 43.61 Mb PubMed search [3] [4]
Wikidata
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Function

NCK belongs to family of adaptor proteins. There are two mammalian NCK genes, NCK1 and NCK2. NCK1 is located in chromosome 3 and NCK2 is located in chromosome 2. The protein contains three SH3 domains and one SH2 domain. The protein has no known catalytic function but has been shown to bind and recruit various proteins involved in the regulation of receptor protein tyrosine kinases. It is through these regulatory activities that this protein is believed to be involved in cytoskeletal reorganization. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.^[7]

Interactions

NCK2 has been shown to interact with:

• Epidermal growth factor receptor,^[5][8][9]
• LIMS1,^[10][8]
• PDGFRB,^[5][9][11]
• PTK2,^[12]
• T-cell surface glycoprotein CD3 epsilon chain^[13] and
• TrkB.^[14]