| Route |
Terminus A |
Terminus B |
First service |
Last service |
Notes |
| 3a |
East Malvern |
Melbourne University |
31 January 2009 |
29 October 2023 |
via St Kilda, ran only on weekends, all services diverted to operate via route 3[17] |
| 4 |
Carnegie |
City (Swanston Street) |
24 June 1934 |
31 October 1970 |
Replaced by route 67 following route number revision[18] |
| 4t |
Malvern East |
Replaced by route 3 following route number revision[18] |
| 7 |
Camberwell |
City (Swanston Street) |
21 November 1929 |
31 October 1970 |
Replaced by route 72 following route number revision.[18] |
| 8 |
Moreland |
Toorak |
17 October 2004 |
30 April 2017 |
Merged into route 58, change associated with the construction of Metro Tunnel[19] |
| 9 |
Northcote |
City (Collins Street) |
1 November 1970 |
14 March 1993 |
via Brunswick Street |
| 10 |
West Preston |
South Melbourne and St Kilda Beach |
25 June 1937 |
29 April 1995 |
Discontinued following elimination of shared depot routes[18] |
| 13 |
West Preston |
City (Spencer Street) |
August 1968 |
26 June 1989 |
via La Trobe Street, discontinued following timetable change at East Preston depot |
| 14 |
East Preston |
City (Spencer Street) |
1 November 1970 |
26 June 1989 |
via La Trobe Street, discontinued following timetable change at East Preston depot |
| 15 |
Brunswick tram depot |
St Kilda Beach |
26 April 1936 |
1 April 1995 |
Split into routes 16 and 22 following elimination of shared depot routes |
| 22 |
Moreland |
Arts Centre |
1 November 1970 |
16 October 2004 |
Merged into route 8 (see above)[20][21] |
| 23 |
Mont Albert |
City (Spencer Street) |
25 September 1972 |
7 September 2001 |
via La Trobe Street |
| 24 |
North Balwyn |
City (Spencer Street) |
25 September 1972 |
26 July 2014 |
via La Trobe Street, discontinued based on the desire to end peak-hour only routes |
| 25 |
Moreland |
Domain Road |
2 April 1995 |
6 October 2000 |
Extension of route 22 that ran only during morning peaks |
| 27 |
Hawthorn |
City (Spencer Street) |
6 June 1934 |
12 February 1965 |
Discontinued following when route 74/75 diverted via Bridge Road to Flinders Street when Hawthorn depot closed[18] |
| 31 |
Hoddle Street |
Victoria Harbour |
25 September 1972 |
26 July 2014 |
Discontinued following network-wide timetable revision |
| 32 |
Camberwell |
City (William Street) |
25 September 1972 |
17 November 1986 |
Discontinued once through-running on William Street routes ceased |
| 33 |
Domain Road |
City (William Street) |
17 November 1986 |
1995 |
Peak hour variant that gradually fell out of use after the closure of South Melbourne depot |
| 34 |
East Melbourne |
City (Spencer Street) |
1995 |
19 September 2003 |
Route 30 became full-time |
| 35 |
Malvern |
City (William Street) |
25 September 1972 |
17 November 1986 |
Discontinued once through-running on William Street routes ceased |
| 36 |
Glen Iris |
| 37 |
Carnegie |
| 38 |
Toorak |
| 42 |
Mont Albert |
City (Collins Street) |
6 June 1934 |
18 December 1993 |
Merged with route 111 into route 109 |
| 55 |
West Coburg |
Domain Interchange |
30 April 1995 |
30 April 2017 |
Merged into route 58. Change associated with the construction of Metro Tunnel[19] |
| 56 |
Brunswick West |
Domain Interchange |
3 February 1946 |
29 April 1995 |
Discontinued to fix route number anomaly with route 55 |
| 57 |
Somerville & Williamstown Rds |
Ballarat Rd & Rosamond Rd |
6 September 1921 |
10 March 1962 |
Discontinued on 10 March 1962, when the Footscray Depot closed and trams ceased operation from there, with only the current Route 82, running out of Essendon Depot, remaining. |
| 58 |
Barkly & Russell Sts |
Footscray station |
| 59 |
Somerville & Williamstown Rds |
Footscray station |
| 60 |
Ballarat & Rosamond Rds |
Footscray station |
|
Gordon & River Sts (Ammunitions Factory) |
Footscray station |
9 November 1941 |
1 May 1954 |
Route ran along Gordon St to River St (also known as the Ammunitions Factory route). On 2 May 1954, it was extended to the Ordnance Factory spur line off Raleigh Rd, to create what is now Route 82 to Moonee Ponds Junction. |
| 68 |
West Coburg |
City (Elizabeth Street) |
1 November 1970 |
31 July 2005 |
Route 55 began seven-day operation |
| 69 |
Kew |
St Kilda Beach |
24 June 1934 |
15 October 2004 |
Merged into route 16[22][21] |
| 74 |
Burwood |
City (Spencer Street) |
9 December 1934 |
18 July 1978 |
Replaced by route 75 following East Burwood extension[18] |
| 77 |
Prahran |
Batman Avenue |
9 December 1934 |
1 November 1986 |
Discontinued due to low patronage/cost-saving[18] |
| 79 |
North Richmond |
St Kilda Beach |
9 December 1934 |
26 July 2014 |
Route 78 became full-time[23] |
| 88 |
East Preston |
City (Bourke Street) |
26 June 1955 |
17 May 1983 |
Replaced by route 86 following Bundoora extension[18] |
| 95 |
Exhibition Street |
Docklands Stadium |
26 June 1955 |
26 July 2014 |
Discontinued based on the desire to end peak-hour only routes |
| 111 |
Exhibition Street |
Port Melbourne |
20 November 1987 |
18 December 1993 |
Merged with route 42 into route 109[18] |
| 112 |
West Preston |
South Melbourne and St Kilda Beach |
April 2000 |
26 July 2014 |
Split into routes 11 and 12 following network-wide timetable revision[23] |