HIS3

The HIS3 gene, found in the Saccharomyces cerevisiae yeast, encodes a protein called Imidazoleglycerol-phosphate dehydratase which catalyses the sixth step in histidine biosynthesis.^[1] It is analogous to hisB in Escherichia coli.

Imidazoleglycerol-phosphate dehydratase
Identifiers
Organism	Saccharomyces cerevisiae
Symbol	HIS3
Entrez	854377
HomoloGene	6979
RefSeq (mRNA)	NM_001183621.1
RefSeq (Prot)	NP_014845.1
UniProt	P06633
Other data
EC number	4.2.1.19
Chromosome	XV: 0.72 - 0.72 Mb
Search for Structures Swiss-model Domains InterPro

Exploits

Mutations in Escherichia coli's analogous gene, hisB allows researchers to select only those individuals expressing the HIS3 gene included on a plasmid. The HIS3 gene is coupled to a certain promoter which can only be activated by successful binding of the relevant transcription factors. This is used in certain methods of bacterial two-hybrid screening to allow the survival of E. coli in which a desired protein-DNA or protein-protein interaction is taking place.^[2]

References

1. "HIS3/YOR202W Summary". Archived from the original on 2006-05-05. Retrieved 2006-11-30.
2. Joung JK, Ramm EI, Pabo CO (June 2000). "A bacterial two-hybrid selection system for studying protein-DNA and protein-protein interactions". Proceedings of the National Academy of Sciences of the United States of America. 97 (13): 7382–7. Bibcode:2000PNAS...97.7382J. doi:10.1073/pnas.110149297. PMC 16554. PMID 10852947.