DLGAP5

Disks large-associated protein 5 (DAP-5) also known as discs large homolog 7 (DLG7) or hepatoma up-regulated protein (HURP) is a protein that in humans is encoded by the DLGAP5 gene.^[5][6][7][8]

DLGAP5
Identifiers
Aliases	DLGAP5, DLG7, HURP, discs large homolog associated protein 5, DLG associated protein 5
External IDs	OMIM: 617859; MGI: 2183453; HomoloGene: 8840; GeneCards: DLGAP5; OMA:DLGAP5 - orthologs
Gene location (Human) Chr. Chromosome 14 (human)[1] Band 14q22.3 Start 55,148,112 bp[1] End 55,191,608 bp[1]
Gene location (Mouse) Chr. Chromosome 14 (mouse)[2] Band 14 C1|14 24.6 cM Start 47,625,236 bp[2] End 47,655,864 bp[2]
RNA expression pattern Bgee Human Mouse (ortholog) Top expressed in secondary oocyte ventricular zone gonad embryo ganglionic eminence trabecular bone testicle bone marrow bone marrow cells sperm Top expressed in tail of embryo zygote genital tubercle primary oocyte yolk sac secondary oocyte ventricular zone blastocyst epiblast fetal liver hematopoietic progenitor cell More reference expression data BioGPS More reference expression data
Gene ontology Molecular function protein binding phosphoprotein phosphatase activity Cellular component microtubule organizing center nucleus cytoskeleton spindle spindle pole centrosome cytoplasm mitochondrion cytosol Biological process positive regulation of mitotic metaphase/anaphase transition cell cycle mitotic chromosome movement towards spindle pole cell population proliferation signaling protein dephosphorylation positive regulation of transcription of Notch receptor target Sources:Amigo / QuickGO
Orthologs Species Human Mouse Entrez 9787 218977 Ensembl ENSG00000126787 ENSMUSG00000037544 UniProt Q15398 Q8K4R9 RefSeq (mRNA) NM_001146015 NM_014750 NM_001145949 NM_144553 RefSeq (protein) NP_001139487 NP_055565 NP_653136 Location (UCSC) Chr 14: 55.15 – 55.19 Mb Chr 14: 47.63 – 47.66 Mb PubMed search [3] [4]
Wikidata
View/Edit Human View/Edit Mouse

DLG7 is a kinetochore protein that stabilizes microtubules in vicinity of chromosomes. DLG7 controls spindle dynamics, promotes interkinetochore tension and efficient kinetochore capture.^[9] DGL7 is a part of Ran-dependent complex.^[10] Stabilization of DGL7 in cell occurs due to phosphorylation by Aurora A kinase.^[11] Expression of DGL7 is found in cancer^[12] and stem cells.^[13]

References

1. GRCh38: Ensembl release 89: ENSG00000126787 – Ensembl, May 2017
2. GRCm38: Ensembl release 89: ENSMUSG00000037544 – Ensembl, May 2017
3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
5. Nomura N, Miyajima N, Sazuka T, Tanaka A, Kawarabayasi Y, Sato S, Nagase T, Seki N, Ishikawa K, Tabata S (Dec 1995). "Prediction of the coding sequences of unidentified human genes. I. The coding sequences of 40 new genes (KIAA0001-KIAA0040) deduced by analysis of randomly sampled cDNA clones from human immature myeloid cell line KG-1". DNA Res. 1 (1): 27–35. doi:10.1093/dnares/1.1.27. PMID 7584026.
6. Nomura N, Miyajima N, Sazuka T, Tanaka A, Kawarabayasi Y, Sato S, Nagase T, Seki N, Ishikawa K, Tabata S (Dec 1995). "Prediction of the coding sequences of unidentified human genes. I. The coding sequences of 40 new genes (KIAA0001-KIAA0040) deduced by analysis of randomly sampled cDNA clones from human immature myeloid cell line KG-1 (supplement)". DNA Res. 1 (1): 47–56. doi:10.1093/dnares/1.1.47. PMID 7584028.
7. "Entrez Gene: DLG7 discs, large homolog 7 (Drosophila)".
8. Bassal S, Nomura N, Venter D, Brand K, McKay MJ, van der Spek PJ (September 2001). "Characterization of a novel human cell-cycle-regulated homologue of Drosophila dlg1". Genomics. 77 (1–2): 5–7. doi:10.1006/geno.2001.6570. PMID 11543626.
9. Wilde A (June 2006). ""HURP on" we're off to the kinetochore!". J. Cell Biol. 173 (6): 829–31. doi:10.1083/jcb.200605150. PMC 2063907. PMID 16785318.
10. Koffa MD, Casanova CM, Santarella R, Köcher T, Wilm M, Mattaj IW (April 2006). "HURP is part of a Ran-dependent complex involved in spindle formation". Curr. Biol. 16 (8): 743–54. Bibcode:2006CBio...16..743K. doi:10.1016/j.cub.2006.03.056. PMID 16631581.
11. Yu CT, Hsu JM, Lee YC, Tsou AP, Chou CK, Huang CY (July 2005). "Phosphorylation and stabilization of HURP by Aurora-A: implication of HURP as a transforming target of Aurora-A". Mol. Cell. Biol. 25 (14): 5789–800. doi:10.1128/MCB.25.14.5789-5800.2005. PMC 1168800. PMID 15987997.
12. Sanderson HS, Clarke PR (June 2006). "Cell biology: Ran, mitosis and the cancer connection". Curr. Biol. 16 (12): R466–8. Bibcode:2006CBio...16.R466S. doi:10.1016/j.cub.2006.05.032. PMID 16782004.
13. Gudmundsson KO, Thorsteinsson L, Sigurjonsson OE, et al. (June 2007). "Gene expression analysis of hematopoietic progenitor cells identifies Dlg7 as a potential stem cell gene". Stem Cells. 25 (6): 1498–506. doi:10.1634/stemcells.2005-0479. PMID 17322106. S2CID 25347875.

Further reading

• Ohara O, Nagase T, Ishikawa K, et al. (1997). "Construction and characterization of human brain cDNA libraries suitable for analysis of cDNA clones encoding relatively large proteins". DNA Res. 4 (1): 53–9. doi:10.1093/dnares/4.1.53. PMID 9179496.
• Bassal S, Nomura N, Venter D, et al. (2001). "Characterization of a novel human cell-cycle-regulated homologue of Drosophila dlg1". Genomics. 77 (1–2): 5–7. doi:10.1006/geno.2001.6570. PMID 11543626.
• Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. Bibcode:2002PNAS...9916899M. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932.
• Tsou AP, Yang CW, Huang CY, et al. (2003). "Identification of a novel cell cycle regulated gene, HURP, overexpressed in human hepatocellular carcinoma". Oncogene. 22 (2): 298–307. doi:10.1038/sj.onc.1206129. PMID 12527899. S2CID 24615395.
• Mantovani F, Banks L (2004). "Regulation of the discs large tumor suppressor by a phosphorylation-dependent interaction with the beta-TrCP ubiquitin ligase receptor". J. Biol. Chem. 278 (43): 42477–86. doi:10.1074/jbc.M302799200. PMID 12902344.
• Laprise P, Viel A, Rivard N (2004). "Human homolog of disc-large is required for adherens junction assembly and differentiation of human intestinal epithelial cells". J. Biol. Chem. 279 (11): 10157–66. doi:10.1074/jbc.M309843200. PMID 14699157.
• Shu H, Chen S, Bi Q, et al. (2004). "Identification of phosphoproteins and their phosphorylation sites in the WEHI-231 B lymphoma cell line". Mol. Cell. Proteomics. 3 (3): 279–86. doi:10.1074/mcp.D300003-MCP200. PMID 14729942.
• Hsu JM, Lee YC, Yu CT, Huang CY (2004). "Fbx7 functions in the SCF complex regulating Cdk1-cyclin B-phosphorylated hepatoma up-regulated protein (HURP) proteolysis by a proline-rich region". J. Biol. Chem. 279 (31): 32592–602. doi:10.1074/jbc.M404950200. PMID 15145941.
• Blot V, Delamarre L, Perugi F, et al. (2005). "Human Dlg protein binds to the envelope glycoproteins of human T-cell leukemia virus type 1 and regulates envelope mediated cell-cell fusion in T lymphocytes". J. Cell Sci. 117 (Pt 17): 3983–93. doi:10.1242/jcs.01266. PMID 15286176. S2CID 23757741.
• Beausoleil SA, Jedrychowski M, Schwartz D, et al. (2004). "Large-scale characterization of HeLa cell nuclear phosphoproteins". Proc. Natl. Acad. Sci. U.S.A. 101 (33): 12130–5. Bibcode:2004PNAS..10112130B. doi:10.1073/pnas.0404720101. PMC 514446. PMID 15302935.
• Zhao L, Qin LX, Ye QH, et al. (2005). "KIAA0008 gene is associated with invasive phenotype of human hepatocellular carcinoma--a functional analysis". J. Cancer Res. Clin. Oncol. 130 (12): 719–27. doi:10.1007/s00432-004-0595-2. PMID 15340842. S2CID 24151704.
• Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC 528928. PMID 15489334.
• Yu CT, Hsu JM, Lee YC, et al. (2005). "Phosphorylation and stabilization of HURP by Aurora-A: implication of HURP as a transforming target of Aurora-A". Mol. Cell. Biol. 25 (14): 5789–800. doi:10.1128/MCB.25.14.5789-5800.2005. PMC 1168800. PMID 15987997.
• Rual JF, Venkatesan K, Hao T, et al. (2005). "Towards a proteome-scale map of the human protein-protein interaction network". Nature. 437 (7062): 1173–8. Bibcode:2005Natur.437.1173R. doi:10.1038/nature04209. PMID 16189514. S2CID 4427026.
• Nousiainen M, Silljé HH, Sauer G, et al. (2006). "Phosphoproteome analysis of the human mitotic spindle". Proc. Natl. Acad. Sci. U.S.A. 103 (14): 5391–6. Bibcode:2006PNAS..103.5391N. doi:10.1073/pnas.0507066103. PMC 1459365. PMID 16565220.
• Wong J, Fang G (2006). "HURP controls spindle dynamics to promote proper interkinetochore tension and efficient kinetochore capture". J. Cell Biol. 173 (6): 879–91. doi:10.1083/jcb.200511132. PMC 2063914. PMID 16769820.
• Beausoleil SA, Villén J, Gerber SA, et al. (2006). "A probability-based approach for high-throughput protein phosphorylation analysis and site localization". Nat. Biotechnol. 24 (10): 1285–92. doi:10.1038/nbt1240. PMID 16964243. S2CID 14294292.
• Olsen JV, Blagoev B, Gnad F, et al. (2006). "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks". Cell. 127 (3): 635–48. doi:10.1016/j.cell.2006.09.026. PMID 17081983.

External links

• DLG7 protein, human at the U.S. National Library of Medicine Medical Subject Headings (MeSH)