C16orf58

Chromosome 16 open reading frame 58, or C16orf58, also known as FLJ13638 is a protein which in humans is encoded by the C16orf58 gene.^[5] The gene itself is 18892 bp long, with mRNA of 2760 bp, and a protein sequence of 468 amino acids. There is a conserved domain of unknown, DUF647. No function has been determined for this gene yet, but it is predicted that it resides in the endoplasmic reticulum in the cytoplasm.^[6]

RUSF1
Identifiers
Aliases	RUSF1, RUS, chromosome 16 open reading frame 58, C16orf58, RUS family member 1
External IDs	MGI: 2384572; HomoloGene: 11232; GeneCards: RUSF1; OMA:RUSF1 - orthologs
Gene location (Human) Chr. Chromosome 16 (human)[1] Band 16p11.2 Start 31,489,471 bp[1] End 31,509,309 bp[1]
Gene location (Mouse) Chr. Chromosome 7 (mouse)[2] Band 7|7 F3 Start 127,870,551 bp[2] End 127,897,342 bp[2]
RNA expression pattern Bgee Human Mouse (ortholog) Top expressed in anterior pituitary left ovary right ovary right lobe of thyroid gland right uterine tube canal of the cervix left lobe of thyroid gland body of pancreas body of uterus right lobe of liver Top expressed in internal carotid artery external carotid artery yolk sac zygote right kidney neural layer of retina epithelium of small intestine proximal tubule genital tubercle secondary oocyte More reference expression data BioGPS n/a
Orthologs Species Human Mouse Entrez 64755 233913 Ensembl ENSG00000140688 ENSMUSG00000030780 UniProt Q96GQ5 Q91W34 RefSeq (mRNA) NM_022744 NM_145590 NM_001360882 RefSeq (protein) NP_073581 NP_663565 NP_001347811 Location (UCSC) Chr 16: 31.49 – 31.51 Mb Chr 7: 127.87 – 127.9 Mb PubMed search [3] [4]
Wikidata
View/Edit Human View/Edit Mouse

Species distribution

C16orf58 has very interesting conservation in that it has orthologs back through plants and fungi. However, it has not been found in reptiles, birds, or amphibians. The below table shows some, but not all, orthologs which were found using BLAST.^[7]

Species	Organism Common Name	NCBI Accession	Sequence identity	E-value	Length (AAs)	Gene Common Name
Homo sapiens	Human	NP_073581	100%	0.0	468	C16orf58
Equus Caballus	Horse	XP_001495510	85%	0.0	468	PREDICTED: similar to UPF0420 protein C16orf58
Canis familiaris	Dog	XP_547054	85%	0.0	485	similar to CG10338-PA
Mus musculus	Mouse	Q91W34	81%	0.0	466	cDNA sequence BC017158
Monodelphis domestica	Opossum	XP_001370394	65%	3e−160	466	PREDICTED: hypothetical protein
Danio rerio	Zebrafish	NP_001103923	53%	4e−112	432	hypothetical protein LOC555936
Drosophila melanogaster	Fly	NP_609897	40%	3e−69	395	CG10338
Arabidopsis thaliana	Thale Cress	AAF81284	37%	2e−68	403	Contains similarity to CG10338 gene product from Drosophila melanogaster
Gallus gallus	Chicken	NP_989823	25%	0.36	1434	protein tyrosine phosphatase, receptor type, U
Xenopus tropicalis	Frog	AAI22058	31%	3.4	268	Stk19 protein
Saccharomyces cerevisiae	Yeast	EDZ73379	25%	0.21	1578	YDL140Cp-like protein
Caenorhabditis elegans	Nematode	NP_502300	19%	3.0	414	hypothetical protein M18.6

Protein Interactions

Though the function is still unknown, C16orf58 has been shown to interact with three different proteins:

• MVD^[8] MVD stands for disphosphomevalonate decarboxylase which is an enzyme which functions in cholesterol biosynthesis.^[9]
• BSCL2^[10] BSCL2 is the Bernardinelli-Seip congenital lipodystrophy 2, or seipin.^[11] It located in the endoplasmic reticulum and is thought to be important in the lipid droplet morphology.
• TSC22D4^[10] The third interacting protein is TSC22D4, TSC22 domain family member 4, and functions as a leucine zipper translational regulation.^[12]

Structure

Although there are several sites that will give predictions on protein structure, C16orf58 does not have a known structure yet. That being said there is at least one transmembrane domain, if not more. Within the protein structure there are several extended areas with uncharged amino acids, these could be possible transmembrane domains, or hydrophobic cores.^[6] The below shows the charge of each of the amino acids in the protein sequence, + for positive, - for negative and 0 for uncharged. Note the large segments of uncharged amino acids appear bolded. These stretches of uncharged amino acids are conserved back through distant orthologs.

      1  00—000-00 000-00000- 0+00+000-0 0000-0000+ 00000+0000 +0-0+-00-0 
     61  0000000000 0000000000 000-0000-0 000000-000 0000000000 0000000000 
    121  0000+00000 0000000+-0 00000+0000 00+00+0-00 0+00+000-0 00-00000-0 
    181  0000000000 000000000+ 0000000000 +00000000+ +0000-000+ -000-00000 
    241  0000000000 0000000000 0000000000 000000+00+ 0000-000-0 +0+000+000 
    301  0+0-00-000 00+0-00000 0000000000 0000+00000 0-00000-00 0-000000-0 
    361  0000000000 0+000+000+ 0000000000 000-00000- 0—0+0+0+0 00++-00000 
    421  +-00-00-00 00+00+000- 000+0-+000 -00-0+0000 000-++00