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Antibody barcoding is a protein profiling technique that has been used to analyze hundreds of proteins from small tissue samples, such as minimally invasive fine-needle aspirates from cancer tissue.[1] Although promising, the technique has not yet been extensively proven or developed.[2]
The method involves optical mapping of DNA sequences that are added as "barcodes" to antibodies attached to the cells in the sample. The method showed high reproducibility and achieved single-cell sensitivity.[1] In addition to profiling cancer cells, the method shows promise as a clinical tool to identify pathway responses to molecularly targeted drugs and to predict drug response in patient samples.[1]
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