Amido black 10B

Amido black 10B is an amino acid staining azo dye used in biochemical research to stain for total protein on transferred membrane blots, such as the western blot.^[2] It is also used in criminal investigations to detect blood present with latent fingerprints - it stains the proteins in blood a blue-black color.^[3] Amido Black can be either methanol or water based as it readily dissolves in both. With picric acid, in a van Gieson procedure, it can be used to stain collagen and reticulin.

Amido black 10B^[1]

Names
IUPAC name Sodium 4-amino-5-hydroxy-3-((E)-(4-nitrophenyl)diazenyl)-6-((E)-phenyldiazenyl)naphthalene-2,7-disulfonate
Other names 4-Amino-5-hydroxy-3-[(4-nitrophenyl)azo]-6-(phenylazo)-2,7-naphthalene disulfonic acid, disodium salt Amidoschwarz Naphthol blue black Acid Black 1 Acidal Black 10B Acidal Navy Blue 3BR Naphthalene Black 10B Buffalo Black NBR C.I. 20470
Identifiers
CAS Number	1064-48-8 Y
3D model (JSmol)	Interactive image
ChEBI	CHEBI:86230 Y
ChemSpider	10660833 Y
ECHA InfoCard	100.012.640
EC Number	213-903-1
PubChem CID	9566044
UNII	SZT789770M N
CompTox Dashboard (EPA)	DTXSID1024415
InChI InChI=1S/C22H16N6O9S2.2Na/c23-19-18-12(11-17(39(35,36)37)21(22(18)29)27-24-13-4-2-1-3-5-13)10-16(38(32,33)34)20(19)26-25-14-6-8-15(9-7-14)28(30)31;;/h1-11,29H,23H2,(H,32,33,34)(H,35,36,37);;/q;2*+1/p-2/b26-25+,27-24+;; Y Key: AOMZHDJXSYHPKS-DROYEMJCSA-L Y InChI=1/C22H16N6O9S2.2Na/c23-19-18-12(11-17(39(35,36)37)21(22(18)29)27-24-13-4-2-1-3-5-13)10-16(38(32,33)34)20(19)26-25-14-6-8-15(9-7-14)28(30)31;;/h1-11,29H,23H2,(H,32,33,34)(H,35,36,37);;/q;2*+1/p-2/b26-25+,27-24+;; Key: AOMZHDJXSYHPKS-NJAWIMKDBA
SMILES [Na+].[Na+].O=N(=O)c1ccc(cc1)/N=N/c2c(N)c4c(cc2S([O-])(=O)=O)cc(c(/N=N/c3ccccc3)c4O)S([O-])(=O)=O
Properties
Chemical formula	C22H14N6Na2O9S2
Molar mass	616.49 g·mol−1
Appearance	Dark red to black
Solubility in water	~ 30 g/L at 20 °C (68 °F)
Hazards
GHS labelling:
Pictograms
Signal word	Warning
NFPA 704 (fire diamond)	2 0 0
Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa). N verify (what is YN ?) Infobox references

See also

• Western blot normalization

References

1. "AMIDO BLACK 10B". chemicalland21.com. Retrieved December 2, 2017.
2. Kurien, B. T., & Scofield, R. H. (2015). Western Blotting: An Introduction. In B. T. Kurien & R. H. Scofield (Eds.), Western Blotting: Methods and Protocols (pp. 17–30). Springer. ISBN 978-1-4939-2694-7
3. Bossers, L. C. A. M., Roux, C., Bell, M., & McDonagh, A. M. (2011). Methods for the enhancement of fingermarks in blood. Forensic Science International, 210(1), 1–11. doi:10.1016/j.forsciint.2011.04.006

External links

• MSDS at Oxford University