Trypan blue
Blue-colored dye / From Wikipedia, the free encyclopedia
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Trypan blue is an azo dye. It is a direct dye for cotton textiles.[3] In biosciences, it is used as a vital stain to selectively colour dead tissues or cells blue.
Quick Facts Names, Identifiers ...
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Names | |
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IUPAC name
(3Z,3'Z)-3,3'-[(3,3'-dimethylbiphenyl-4,4'-diyl)di(1Z)hydrazin-2-yl-1-ylidene]bis(5-amino-4-oxo-3,4-dihydronaphthalene-2,7-disulfonic acid) | |
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3D model (JSmol) |
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ChEBI | |
ChEMBL | |
ChemSpider |
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ECHA InfoCard | 100.000.715 ![]() |
KEGG |
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PubChem CID |
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UNII | |
CompTox Dashboard (EPA) |
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Properties | |
C34H24N6Na4O14S4 | |
Molar mass | 872.88 |
Appearance | deep blue in aqueous solution[1] |
Melting point | > 300 °C (572 °F; 573 K) |
10mg/ml [2] | |
Solubility | 20 mg/mL in methyl Cellosolve, and 0.6 mg/mL in ethanol |
Pharmacology | |
S01KX02 (WHO) | |
Hazards | |
Lethal dose or concentration (LD, LC): | |
LD50 (median dose) |
6200 mg/kg (oral, rat) |
Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa).
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Live cells or tissues with intact cell membranes are not coloured. Since cells are very selective in the compounds that pass through the membrane, in a viable cell trypan blue is not absorbed; however, it traverses the membrane in a dead cell. Hence, dead cells appear as a distinctive blue colour under a microscope. Since live cells are excluded from staining, this staining method is also described as a dye exclusion method.