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Insect cell line From Wikipedia, the free encyclopedia
Sf9 cells, a clonal isolate of Spodoptera frugiperda Sf21 cells (IPLB-Sf21-AE), are commonly used in insect cell culture for recombinant protein production using baculovirus. They were originally established from ovarian tissue.[1] They can be grown in the absence of serum, and can be cultured attached or in suspension.[2]
It has previously been shown that some Sf9 cell lines harbor a negative sense Rhabdovirus called Spodoptera frugiperda rhabdovirus (SfRV).[3][4] However, not all tested Sf9 cells appear to be infected with this virus.[5] SfRV appears to be insect-specific and does not appear to infect mammalian cell lines.[6]
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