Loop-mediated isothermal amplification
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Loop-mediated isothermal amplification (LAMP) is a single-tube technique for the amplification of DNA[1] and a low-cost alternative to detect certain diseases that was invented in 2000 at the University of Tokyo.[2] Reverse transcription loop-mediated isothermal amplification (RT-LAMP) combines LAMP with a reverse transcription step to allow the detection of RNA.
This article is missing information about primer design, loop formation. (March 2019) |
LAMP is an isothermal nucleic acid amplification technique. In contrast to the polymerase chain reaction (PCR) technology, in which the reaction is carried out with a series of alternating temperature steps or cycles, isothermal amplification is carried out at a constant temperature, and does not require a thermal cycler.